Cyto-Cal™ Count Control - FAQs

查看更多产品信息 Cyto-Cal™ Count Control - FAQs (FC7)

4 个常见问题解答

我用计数微珠进行细胞计数，但在散点图上找不到微珠，该怎么办？

首先检查您的阈值，看它是否设置为前向散射。如果是的话，微珠可能被阈值排除。降低阙值设置后，应该就可以显示出您的微珠了。

我想用流式细胞仪计数细胞，我该怎么做？

可以向样品中添加内部微球计数的标准品来完成流式细胞仪细胞计数。收集到的微球数量已知体积。这使得您可以计算细胞浓度。

I am using counting beads to count cells, but I cannot find the beads on my scatter plots. What do I do?

The first thing to do is check your threshold and see if it is set on forward scatter. If so, the beads are probably being excluded by the threshold. Reducing the threshold setting should reveal your beads.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to count my cells using flow cytometry. How do I do this?

Cell counting using flow cytometry can be accomplished by adding an internal microsphere counting standard to the flow cytometric sample. The number of reference beads that are collected reflects a known volume. This allows you to calculate cell concentration.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.