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E-Gel™ CloneWell™ Agarose Gels with SYBR Safe™ DNA Gel Stain, 0.8% + E-Gel™ iBase™ Power System + E-Gel™ Safe Imager™ Transilluminator Starter Kit (UK only) - FAQs

查看更多产品信息 E-Gel™ CloneWell™ Agarose Gels with SYBR Safe™ DNA Gel Stain, 0.8% + E-Gel™ iBase™ Power System + E-Gel™ Safe Imager™ Transilluminator Starter Kit (UK only) - FAQs (G6500STUK)

9 个常见问题解答

使用E-Gel SizeSelect 或 E-Gel CloneWell 凝胶时我的DNA不小心跑过了收集孔，我该怎么办？

你可以使用Reverse E-Gel 程序反向电泳使条带跑回到收集孔内。

和iBase电源系统配套使用的E-Gel Safe Imager实时透射仪不工作了。我应该怎么办？

请检查iBase电源系统的电源线。PowerBase v.4系统和E-Gel Safe Imager实时透射仪的电源线经常被混淆。如果用错电源线，虽然风扇和LED灯将正常工作，但蓝光灯将不会工作。你需要使用iBase电源线，它上面写有“48V”。

E-Gel Safe Imager实时透射仪（货号G6500）与Safe Imager 2.0蓝光透射仪（货号G6600）的差别是什么？

E-Gel Safe Imager实时透射仪加载到E-Gel iBase电源系统上，可对在含有SYBR Safe染料的E-Gel琼脂糖凝胶上DNA/RNA的迁移进行实时观察。Safe Imager 2.0蓝光透射仪是专为在实验台上观察已染色凝胶而设计的独立系统。

在第二排胶孔中加入水对于E-Gel SizeSelect琼脂糖凝胶和E-Gel CloneWell 琼脂糖凝胶很重要吗？

是的，在您感兴趣的条带跑入收集孔前请确保第二排胶孔已加入无菌水。请注意：各孔再填充的体积可能会不同。不要加过满而溢出。

我想在电泳后回收DNA片段，有什么推荐？

您可以使用我们的E-Gel SizeSelect 琼脂糖凝胶及E-Gel CloneWell琼脂糖凝胶，它是双排胶孔的预制琼脂糖凝胶，提供简化的DNA回收方法。将您的样本加入上排胶孔并进行电泳，直到您的条带或所需大小范围的片段进入下排胶孔。然后使用加样枪吸出选定大小的DNA。不需要额外的凝胶纯化步骤。

The E-Gel Safe Imager Real-Time Transilluminator for the iBase Power System is not working. What should I do?

Please check the cord you are using for the iBase Power System. Oftentimes, the power cords for the PowerBase v.4 system and the E-Gel Safe Imager Real-Time Transilluminator get mixed up. If this is the case, the blue light will not come on even though the fan and LED light will operate. You will need to use the iBase cord, which should say 48 V on it.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the difference between the E-Gel Safe Imager Real-Time Transilluminator (Cat. No. G6500) and the Safe Imager 2.0 Blue-Light Transilluminator (Cat. No. G6600)?

The E-Gel Safe Imager Real-Time Transilluminator docks onto the E-Gel iBase Power System and allows real-time imaging of the migration of DNA/RNA in E-Gel Agarose Gels containing SYBR Safe stain. The Safe Imager 2.0 Blue-Light Transilluminator is an independent system designed for viewing stained gels on the benchtop.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Is it important to include water in the second row of wells for the E-Gel SizeSelect Agarose Gels and E-Gel CloneWell Agarose Gels?

Yes, please ensure that the second row is filled with sterile water prior to running your band of interest into the collection well. Please note that the refill volume may vary between wells. Do not overfill.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

I would like to isolate DNA after gel electrophoresis. What do you recommend?

We offer our E-Gel SizeSelect Agarose Gels as well as our E-Gel CloneWell Agarose Gels, which are double-comb, precast agarose gels with simplified DNA recovery. Load your sample into the top row and electorphorese until your band or desired size range enters the bottom row. Then, easily remove the size-selected DNA with a pipette. No additional gel purification steps are necessary.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.