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E-Gel™ Power Snap Electrophoresis System Starter Kit, EX DC 1% - FAQs

E-Gel™ Power Snap Electrophoresis System Starter Kit, EX DC 1% - FAQs

查看更多产品信息 E-Gel™ Power Snap Electrophoresis System Starter Kit, EX DC 1% - FAQs (G8331ST)

9 个常见问题解答

我在进行E-Gel EX琼脂糖凝胶电泳时没有电流。这是为什么？

这里是胶跑得不正常的一些常见原因：

•底座的铜电极因为不当使用而损坏。请确保底座的铜电极是完好的。
•您使用的胶盒已经过期或有瑕疵。
•E-Gel EX胶盒没有正确的插入底座。
•您使用了错误的适配器。

E-Gel 琼脂糖凝胶与E-Gel EX琼脂糖凝胶的区别是什么？

E-Gel EX琼脂糖凝胶能更快分离DNA，且具有更高的灵敏度，能提供额外的灵活性。EX凝胶染料是专利技术（并非基于SYBR 技术），尽管它与SYBR染料拥有相同的光谱特性。电泳时，E-Gel EX凝胶需要使用E-Gel iBase电源系统，而E-Gel琼脂糖凝胶既可使用PowerBase v.4也可使用E-Gel iBase电源系统。E-Gel EX凝胶的灵敏度比溴化乙啶高5倍。

I am not seeing any current when I try to run my E-Gel EX agarose gels. Why is this?

Here are some common reasons why your gel is not running properly:

- Copper contacts in the base are damaged due to improper use. Make sure the copper contacts in the base are intact.
- An expired or defective gel cassette was used.
- The E-Gel EX cassette was not inserted properly into a base.
- An incorrect adaptor was used.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the special feature of E-Gel EX agarose gels?

E-Gel EX agarose gels separate DNA faster, offer enhanced sensitivity, and provide added flexibility. The stain within the EX gels is proprietary (not based on SYBR technology), though it has the same spectral properties as SYBR stains. The E-Gel EX gels have a sensitivity that is 5X greater than gels using ethidium bromide.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

I have an E-Gel Simple Runner Electrophoresis Device in my lab, are the new 11-well and 22-well E-Gel agarose gels compatible with it?

Due to the differences in dimensions, the new E-Gel 11-well and 22-well agarose gel formats are not compatible with the E-Gel Simple Runner Electrophoresis Device.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Which DNA ladders do you recommend using with E-Gel EX agarose gels and E-Gel agarose gels with SYBR Safe stain?

To select the DNA ladder that yields the best resolution for your specific E-Gel, please refer to the table on page 45 of the E-Gel Power Snap Electrophoresis System user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017050_egel_powersnapsystem_UG.pdf).

Can I use double comb E-Gel EX agarose gels or E-Gel agarose gels with SYBR Safe stain for RNA analysis?

E-Gel EX Double Comb agarose gels and E-Gel Double Comb agarose gels with SYBR Safe stain have not been tested for use in RNA applications. We recommend using E-Gel EX Single Comb agarose gels with 11 wells for RNA analysis.

Can I dispose of E-Gel EX agarose gels the same way as E-Gel agarose gels with SYBR Safe DNA Gel Stain?

No. Standard safety and hazardous waste disposal procedures should be followed when handling E-Gel EX agarose gels.

What is the difference between E-Gel agarose gels with SYBR Safe DNA Gel Stain and E-Gel EX agarose gels?

E-Gel EX agarose gels separate DNA faster, offer enhanced sensitivity, and provide added flexibility. The stain within the E-Gel EX agarose gels is SYBR Gold II which has similar spectral properties but increased sensitivity compared to SYBR Safe DNA Gel Stain. E-Gel EX agarose gels are especially suited for applications where high sensitivity is critical.