FIX & PERM™ Cell Permeabilization Kit, 50 tests - FAQs

View additional product information for FIX & PERM™ Cell Permeabilization Kit - FAQs (GAS003, GAS004)

9 product FAQs found

FIX & PERM细胞透化试剂盒实验方案没有说明我应向试管中加多少体积的细胞，我该如何做？

您应该加入约100 µL的细胞。为此，方法之一是分装正确数量的细胞（10E6），然后离心去上清。残留体积大约为100 µL。

我能使用FIX & PERM细胞通透试剂盒暴露细胞核表位吗？

可以。通常情况下，您需要延长在通透缓冲液（试剂B）中的孵育时间，以下为两份相关的文献：

•Millard I, Degrave E, Philippe M et al. (1998) Detection of intracellular antigens by flow cytometry: comparison of two chemical methods and microwave heating. Clin Chem 44:2320–2330.
•Parker JE, Mufti GJ, Rasool F et al. (2000) The role of apoptosis, proliferation, and the Bcl-2–related proteins in the myelodysplastic syndromes and acute myeloid leukemia secondary to MDS. Blood 96:3932–3938.

FIX & PERM细胞通透试剂盒可为流式细胞仪准备细胞；我能将其用于免疫组织化学么？

可以，以下为两份相关的文献：

Haranaga S, Yamaguchi H, Friedman H et al.(2001) Chlamydia pneumoniae infects and multiplies in lymphocytes In vitro. Infect Immun 69:7753–7759.
Al-Mufti R, Hambley H, Albaiges G et al. (2000) Increased fetal erythroblasts in women who subsequently develop pre-eclampsia. Hum Reprod 15:1624–1628.

FIX & PERM细胞通透试剂盒（货号GAS003和GAS004）会裂解红细胞么？

会。在流式细胞术应用中，FIX & PERM细胞通透试剂盒可用于固定和通透悬浮细胞，从而使抗体可以接近到胞内靶标且不降低其散射特性。此试剂盒经验证可用于细胞系、全血和骨髓。除了固定和通透细胞外，该试剂盒还可裂解红细胞。其可作为通用标记试剂（GPR），可用于临床应用，同时也可广泛用于实验室研究。

Is the FIX & PERM Cell Permeabilization Kit (Cat. No. GAS003, GAS004) compatible with conjugates (dyes) besides FITC and PE?

Yes, this product is compatible with other conjugated antibodies, outside of FITC and PE.

The FIX & PERM Cell Permeabilization Kit protocol does not say what volume my cells should be in when they are added to the tube. What should I do?

Your cells should be in approximately 100 µL volume. One way to do this is to aliquot the correct number of cells (10E6) and then spin them down and decant the supernatant. The residual volume should be approximately 100 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the FIX & PERM Cell Permeabilization Kit to access nuclear epitopes?

Yes. Generally, you need to increase the incubation time in the permeabilization buffer (Reagent B). Here are a couple of references:
-Millard I, Degrave E, Philippe M et al. (1998) Detection of intracellular antigens by flow cytometry: comparison of two chemical methods and microwave heating. Clin Chem 44:2320-2330(http://www.ncbi.nlm.nih.gov/pubmed/9799760).
-Parker JE, Mufti GJ, Rasool F et al. (2000) The role of apoptosis, proliferation, and the Bcl-2-related proteins in the myelodysplastic syndromes and acute myeloid leukemia secondary to MDS. Blood 96:3932-3938(http://www.ncbi.nlm.nih.gov/pubmed/11090080).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The FIX & PERM Cell Permeabilization Kit is used to prepare cells for flow cytometry; can I use it for immunohistochemistry?

Yes. Here are a couple of references:
Haranaga S, Yamaguchi H, Friedman H et al.(2001) Chlamydia pneumoniae infects and multiplies in lymphocytes In vitro. Infect Immun 69:7753-7759 (http://www.ncbi.nlm.nih.gov/pubmed/11705957).

Al-Mufti R, Hambley H, Albaiges G et al. (2000) Increased fetal erythroblasts in women who subsequently develop pre-eclampsia. Hum Reprod 15:1624-1628 (http://www.ncbi.nlm.nih.gov/pubmed/10875878).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does the FIX & PERM Cell Permeabilization Kit (Cat. Nos. GAS003 and GAS004) lyse red blood cells?

Yes. The FIX & PERM Cell Permeabilization Kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing access of antibody to intracellular targets without sacrificing scatter characteristics. It has been validated for use in cell lines, whole blood, and bone marrow. In addition fixing and permeabilizing cells, it lyses red blood cells. It is labeled as a general purpose reagent (GPR) and can be used in clinical settings, but it is also used widely in research labs.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.