Control Vesicles
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Gibco™

Control Vesicles

These Control Vesicles are insect-derived purified plasma membranes with no inserted transport proteins for use as a negative control inRead more
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Catalog NumberQuantity
GM00030.5 mL
Catalog number GM0003
Price (CNY)
14,384.00
Each
Add to cart
Quantity:
0.5 mL
Price (CNY)
14,384.00
Each
Add to cart
These Control Vesicles are insect-derived purified plasma membranes with no inserted transport proteins for use as a negative control in vesicular transport assays. Our Control Vesicles control for nonspecific absorption and transport of test substrates to insect plasma membranes.

Control Vesicle Details
Control Vesicles are a negative control product for use with ABC transporter vesicles. They are prepared from purified plasma membranes isolated from an insect cell system (Sf9 cells). Due to the nonspecific transport activity and absorption properties of test substrates to Sf9 cell membranes use of these Control Vesicles is highly recommended.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Pharma and Biopharma
GradeResearch Grade
Host SpeciesS. frugiperda, Spodoptera frugiperda
PackagingVial
Product TypeControl Vesicles
Quantity0.5 mL
Shipping ConditionDry Ice
SourceS. frugiperda,Spodoptera frugiperda
FormFrozen
Unit SizeEach
Contents & Storage
Store Control Vesicles at -80°C.

Frequently asked questions (FAQs)

What is the supplied quantity of ATP-binding cassette (ABC) Transporter Vesicles?

The quantity supplied is 2.5 mg total protein/500 µL per vial. The protein concentration is 5 mg/mL. When our recommended protocol is followed, 50 vesicular transport assays or 125 ATPase assays can be performed with one vial of ABC Transporter Vesicles product.

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.

What is the storage condition for ATP-binding cassette (ABC) Transporter Vesicles?

We recommend storing them at -80 degrees C.

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.

What are ATP-binding cassette (ABC) Transporter Vesicles?

Prepared from Sf9 cells which have been engineered to over-express specific ABC transporters, ATP-binding cassette (ABC) Transporter Vesicles are “inside-out” vesicles designed for use in vesicular transport assays. They allow the investigation of possible interactions between specific ABC transporters and test compounds. ABC Transporter Vesicles provide high levels of transporter activity with low background, giving a clear signal if the test compound is a substrate or inhibitor of a specific efflux transporter.

While ABC transporters typically mediate the efflux of substrates from cells, transporters expressed on these inside-out vesicles import substrates into the vesicles. It is therefore possible to quantitatively evaluate transport activity for the test compound by determining the amount incorporated into the vesicles.
Note: One vial of vesicle product can perform 50 vesicular transport assays.

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.