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Gibco™

对照囊泡

对照囊泡
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货号数量
GM00030.5 mL
货号 GM0003
价格(CNY)
14,406.00
Each
数量:
0.5 mL
价格(CNY)
14,406.00
Each
这些对照囊泡是昆虫衍生的纯化质膜,无插入转运蛋白,用作囊泡转运检测中的阴性对照品。我们的对照囊泡控制试验底物的非特异性吸收和转运至昆虫质膜。

对照囊泡详细信息
对照囊泡是与 ABC 转运体囊泡配合使用的阴性对照产品。对照囊泡由从昆虫细胞系统(Sf9 细胞)分离的纯化质膜制备。由于试验底物对 Sf9 细胞膜的非特异性转运活性和吸收特性,强烈建议使用这些对照囊泡。

仅供研究使用。不适用于动物或人类的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
适用于(应用)制药和生物制药
分级研究级
宿主种属草地贪夜蛾 (S. frugiperda, Spodoptera frugiperda)
包装管号
产品类型对照囊泡
数量0.5 mL
运输条件干冰
来源草地贪夜蛾 (S. frugiperda,Spodoptera frugiperda)
形式冰冻
Unit SizeEach
内容与储存
对照囊泡储存在 -80°C 下。

常见问题解答 (FAQ)

What is the supplied quantity of ATP-binding cassette (ABC) Transporter Vesicles?

The quantity supplied is 2.5 mg total protein/500 µL per vial. The protein concentration is 5 mg/mL. When our recommended protocol is followed, 50 vesicular transport assays or 125 ATPase assays can be performed with one vial of ABC Transporter Vesicles product.

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.

What is the storage condition for ATP-binding cassette (ABC) Transporter Vesicles?

We recommend storing them at -80 degrees C.

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.

What are ATP-binding cassette (ABC) Transporter Vesicles?

Prepared from Sf9 cells which have been engineered to over-express specific ABC transporters, ATP-binding cassette (ABC) Transporter Vesicles are “inside-out” vesicles designed for use in vesicular transport assays. They allow the investigation of possible interactions between specific ABC transporters and test compounds. ABC Transporter Vesicles provide high levels of transporter activity with low background, giving a clear signal if the test compound is a substrate or inhibitor of a specific efflux transporter.

While ABC transporters typically mediate the efflux of substrates from cells, transporters expressed on these inside-out vesicles import substrates into the vesicles. It is therefore possible to quantitatively evaluate transport activity for the test compound by determining the amount incorporated into the vesicles.
Note: One vial of vesicle product can perform 50 vesicular transport assays.

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.