Myeloperoxidase: a myeloid cell nuclear antigen with DNA-binding properties.
AuthorsMurao S, Stevens FJ, Ito A, Huberman E
JournalProc Natl Acad Sci U S A
PubMed ID2829220
An antigen histochemically localized in the nuclei and cytoplasmic granules of normal and leukemic human myeloid cells has been identified as myeloperoxidase (MPO; EC 1.11.1.7). The localization and amount of the enzyme was determined by using a murine monoclonal antibody designated H-43-5 raised against nuclear proteins derived from human promyelocytic ... More
Myeloperoxidase: its structure and expression during myeloid differentiation.
AuthorsKoeffler HP, Ranyard J, Pertcheck M
JournalBlood
PubMed ID2981591
Myeloperoxidase (MPO) is a major protein present in myeloid cells and is used by these cells to help kill microbes. The human promyelocytic HL-60 line can be induced to differentiate to granulocytes or macrophagelike cells. Poly (A) containing RNA was isolated from HL-60 granulocytes, HL-60 macrophages, HL-60 blasts, and normal ... More
Comparative analysis of different permeabilization methods for the flow cytometry measurement of cytoplasmic myeloperoxidase and lysozyme in normal and leukemic cells.
AuthorsLanza F, Latorraca A, Moretti S, Castagnari B, Ferrari L, Castoldi G
JournalCytometry
PubMed ID9222099
Using a direct one-color (fluorescein isothiocyanate; FITC) staining method with a Facscan flow cytometer, we evaluated the intracellular expression of two granular constituents of myeloid cells [myeloperoxidase (MPO) and lysozyme] on leukemic cells from 21 patients with acute myeloid leukemia (AML), and 6 patients with acute lymphoblastic leukemia (ALL). Three ... More
Flow cytometric detection of intracellular antigens for immunophenotyping of normal and malignant leukocytes.
AuthorsGroeneveld K, te Marvelde JG, van den Beemd MW, Hooijkaas H, van Dongen JJ
JournalLeukemia
PubMed ID8709649
Intracellular antigens are of major importance for immunophenotyping of normal leukocytes as well as leukemias and malignant lymphomas. Immunofluorescence microscopic evaluation of cytocentrifuge preparations has remained the preferred technique for detection of intracellular antigens for a long time. Recently, flow cytometric detection of intracellular antigens has been improved by the ... More