Ponasterone A - FAQs

Ecdysone载体与细胞系已经停产了，不过我们仍继续提供诱导剂米乐甾酮A与松甾酮A。

The Ecdysone vectors and cell lines have been discontinued, but we do still offer the inducers, Muristerone A and Ponasterone A.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

We do not have an exact measurement for the half-life of Ponasterone A in media. However, in studies measuring the “decay period” of expression when inducing with Ponasterone A, peak activity was seen at 24 hours post induction. There was a 30% decrease in activity at 48 hours post induction and a 70% decrease after 72 hours. Based on this data, we recommend replenishment of medium every 2-3 days with fresh inducer, which will usually correspond to cell splitting times.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Ponasterone A solution may be prepared as 5 mM stock in ethanol (250 µg of Ponasterone A in 100µL of ethanol). They will take a while to go into solution. Add ethanol and incubate at 37 degrees C for about 15 minutes, then vortex vigorously. Ponasterone A is also highly soluble in DMSO.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

The molecular weight of Ponasterone A is 464.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Our vectors have not been completely sequenced. Your sequence data may differ when compared to what is provided. Known mutations that do not affect the function of the vector are annotated in public databases.

No, our vectors are not routinely sequenced. Quality control and release criteria utilize other methods.

Sequences provided for our vectors have been compiled from information in sequence databases, published sequences, and other sources.