'Ribonuclease L (RNase L), the 2'',5''-oligoadenylate-dependent ribonuclease, is one of the cellular antiviral systems with enhanced activity in the presence of interferon. A reaction scheme has been developed to model the sequence of steps necessary for the activation of RNase L (Cole, J. L., Carroll, S. S., Blue, E. S., ... More
Design and development of a fluorescent probe for monitoring hydrogen peroxide using photoinduced electron transfer.
AuthorsSoh N, Sakawaki O, Makihara K, Odo Y, Fukaminato T, Kawai T, Irie M, Imato T,
JournalBioorg Med Chem
PubMed ID15670921
A novel fluorescent probe, 7-hydroxy-2-oxo-N-(2-(diphenylphosphino)ethyl)-2H-chromene-3-carboxamide (DPPEA-HC) was developed for use in monitoring hydrogen peroxide (H2O2) production. DPPEA-HC, which consists of a diphenylphosphine moiety and a 7-hydroxycoumarin moiety, reacts with H2O2 to form DPPEA-HC oxide, which is analogous to the reaction of triphenylphosphine with hydroperoxides such as H2O2 to form triphenylphosphine ... More
Crocus sativus lectin recognizes Man3GlcNAc in the N-glycan core structure.
AuthorsOda Y, Nakayama K, Abdul-Rahman B, Kinoshita M, Hashimoto O, Kawasaki N, Hayakawa T, Kakehi K, Tomiya N, Lee YC
JournalJ Biol Chem
PubMed ID10837483
Crocus sativus lectin (CSL) is one of the truly mannose-specific plant lectins that has a unique binding specificity that sets it apart from others. We studied sugar-binding specificity of CSL in detail by a solution phase method (fluorescence polarization) and three solid phase methods (flow injection, surface plasmon resonance, and ... More
Activation of RNase L by 2',5'-oligoadenylates. Biophysical characterization.
AuthorsCole JL, Carroll SS, Blue ES, Viscount T, Kuo LC
JournalJ Biol Chem
PubMed ID9235909
Ribonuclease L (RNase L) is an endoribonuclease that is activated upon binding of adenosine oligomers linked 2' to 5' to cleave viral and cellular RNAs. We recently proposed a model for activation in which activator A binds to monomer, E, to form EA, which subsequently dimerizes to the active form, ... More