Morphogenetic domains in the yolk syncytial layer of axiating zebrafish embryos.
AuthorsD'Amico LA, Cooper MS
JournalDev Dyn
PubMed ID11748830
'The yolk syncytial layer (YSL) of the teleostean yolk cell is known to play important roles in the induction of cellular mesendoderm, as well as the patterning of dorsal tissues. To determine how this extraembryonic endodermal compartment is subdivided and morphologically transformed during early development, we have examined collective movements ... More
A cell-free system for light-dependent nuclear import of phytochrome.
AuthorsPfeiffer A, Kunkel T, Hiltbrunner A, Neuhaus G, Wolf I, Speth V, Adam E, Nagy F, Schäfer E,
JournalPlant J
PubMed ID18980642
Translocation from the cytosol to the nucleus is an essential step in phytochrome (phy) signal transduction. In the case of phytochrome A (phyA), this step occurs with the help of FHY1 (far-red-elongated hypocotyl 1), a specific transport protein. To investigate the components involved in phyA transport, we used a cell-free ... More
Coordinated cell-shape changes control epithelial movement in zebrafish and Drosophila.
AuthorsKöppen M, Fernández BG, Carvalho L, Jacinto A, Heisenberg CP,
JournalDevelopment
PubMed ID16794032
Epithelial morphogenesis depends on coordinated changes in cell shape, a process that is still poorly understood. During zebrafish epiboly and Drosophila dorsal closure, cell-shape changes at the epithelial margin are of critical importance. Here evidence is provided for a conserved mechanism of local actin and myosin 2 recruitment during theses ... More
Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells.
AuthorsShalek AK, Robinson JT, Karp ES, Lee JS, Ahn DR, Yoon MH, Sutton A, Jorgolli M, Gertner RS, Gujral TS, Macbeath G, Yang EG, Park H,
JournalProc Natl Acad Sci U S A
PubMed ID20080678
A generalized platform for introducing a diverse range of biomolecules into living cells in high-throughput could transform how complex cellular processes are probed and analyzed. Here, we demonstrate spatially localized, efficient, and universal delivery of biomolecules into immortalized and primary mammalian cells using surface-modified vertical silicon nanowires. The method relies ... More
Control of directed cell migration in vivo by membrane-to-cortex attachment.
Authors
JournalPLoS Biol
PubMed ID21151339
Movement directionality in collective migration of germ layer progenitors.
Authors
JournalCurr Biol
PubMed ID20079641
Histones to the cytosol: exportin 7 is essential for normal terminal erythroid nuclear maturation.
Authors
JournalBlood
PubMed ID25092175
Control of convergent yolk syncytial layer nuclear movement in zebrafish.