Analysis of gene expression by in situ hybridization on adult zebrafish brain sections.
AuthorsKühn E, Köster RW,
JournalCSH Protoc
PubMed ID20150145
Whole-mount in situ hybridization is a powerful method for the analysis of gene expression in zebrafish. However, the thickness of adult brain tissue often hampers penetration of reagents, making the direct application of protocols normally used for embryonic stages on adult tissues difficult. This protocol presents a detailed procedure for ... More