A protein microarray-based analysis of S-nitrosylation.
AuthorsFoster MW, Forrester MT, Stamler JS,
JournalProc Natl Acad Sci U S A
PubMed ID19864628
The ubiquitous cellular influence of nitric oxide (NO) is exerted substantially through protein S-nitrosylation. Whereas NO is highly promiscuous, physiological S-nitrosylation is typically restricted to one or very few Cys residue(s) in target proteins. The molecular basis for this specificity may derive from properties of the target protein, the S-nitrosylating ... More
Analysis of gene expression by in situ hybridization on adult zebrafish brain sections.
AuthorsKühn E, Köster RW,
JournalCSH Protoc
PubMed ID20150145
Whole-mount in situ hybridization is a powerful method for the analysis of gene expression in zebrafish. However, the thickness of adult brain tissue often hampers penetration of reagents, making the direct application of protocols normally used for embryonic stages on adult tissues difficult. This protocol presents a detailed procedure for ... More
Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos.
AuthorsPáldi K, Mosiolek M, Nodine MD
JournalMethods Mol Biol
PubMed ID31975297
'Small RNAs mediate posttranscriptional gene silencing in plants and animals. This often occurs in specific cell or tissue types and can be necessary for their differentiation. Determining small RNA (sRNA) localization patterns at cellular resolution can therefore provide information on the corresponding gene regulatory processes they are involved in. Recent ... More