流式细胞分析用低氧绿色试剂
流式细胞分析用低氧绿色试剂
Thermo Scientific™

流式细胞分析用低氧绿色试剂

Hypoxia Green Reagent for Flow Cytometry is an antibody-independent reagent used to detect low levels of oxygen levels in live了解更多信息
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货号数量
H200352 x 50 assays
货号 H20035
价格（CNY)
3,664.00
飞享价
Ends: 31-Dec-2025
4,868.00
共减 1,204.00 (25%)
Each
添加至购物车
数量:
2 x 50 assays
价格（CNY)
3,664.00
飞享价
Ends: 31-Dec-2025
4,868.00
共减 1,204.00 (25%)
Each
添加至购物车
Hypoxia Green Reagent for Flow Cytometry is an antibody-independent reagent used to detect low levels of oxygen levels in live cells. The membrane-permeant probe releases rhodamine as oxygen levels decrease, resulting in a fluorogenic response. Hypoxia is a characteristic of many diseases, including cardiovascular disease and tumor-mediated immunosuppression and is critical to tumor survival and growth.

• Sensitive, fixed, end-point assay
• Detects decreases in oxygen in live cells
• Fluorogenic, non-antibody based probe

规格
检测方法荧光
产品规格管装
数量2 x 50 assays
运输条件室温
发射488
适用于（设备）流式细胞仪
产品类型试剂名称
Unit SizeEach
内容与储存
2 小瓶，每瓶 50 次检测
  • 保存在 -5°C 到 -30°C。

    • 常见问题解答 (FAQ)

    Can the Hypoxia Green Reagent for Flow Cytometry be used in cells expressing fluorescent proteins?

    Yes, as long as the fluorescent protein does not emit in the green range (˜510–535 nm). For cells that do not express a fluorescent protein, we recommend viewing unstained cells under the FITC channel to examine autofluorescence. For cells expressing a fluorescent protein, we recommend analyzing unstained cell samples to determine the extent the fluorescent protein emission may overlap in the FITC channel.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Our Hypoxia Green Reagent for Flow Cytometry is already highly fluorescent before using the product. What could have caused this?

    This could be due to storage of the product under anaerobic or low oxygen conditions. The Hypoxia Green Reagent for Flow Cytometry increases in fluorescence upon exposure to low oxygen environments, and this change is not reversible. For some ROS indicators, we recommend storing the reagent under dry nitrogen or argon to prevent oxidation during storage. However, this is not appropriate for the Hypoxia Green Reagent for Flow Cytometry.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    What is different between the use of the Hypoxia Green Reagent for Flow Cytometry (Cat No. H20035) versus imaging using the Image-iT Green Hypoxia Reagent (Cat Nos. I14833, I14834)?

    The difference between these products is in the final working concentration of the reagent and incubation time. For flow cytometry applications, we recommend using a final concentration in the range of 0.5 to 1 µM with an incubation time ranging from 2 to 3 hrs. For imaging, we recommend a final concentration in the range of 1 to 10 µM with an incubation time ranging from 30 mins to 1 hr. For both applications, one should optimize the final working concentration and incubation time.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Is Hypoxia Green Reagent for Flow Cytometry (Cat No. H20035) the same reagent as the Image-iT Green Hypoxia Reagent (Cat Nos. I14833, I14834)?

    The Hypoxia Green Reagent for Flow Cytometry and the Image-iT Green Hypoxia Reagent are the same reagent, formulated differently for flow cytometry and imaging use, respectively.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.