Hypoxia Green Reagent for Flow Cytometry
Hypoxia Green Reagent for Flow Cytometry
Thermo Scientific™

Hypoxia Green Reagent for Flow Cytometry

Hypoxia Green Reagent for Flow Cytometry is an antibody-independent reagent used to detect low levels of oxygen levels in liveRead more
Have Questions?
Catalog NumberQuantity
H200352 x 50 assays
Catalog number H20035
Price (CNY)
3,664.00
Online Exclusive
Ends: 31-Dec-2025
4,868.00
Save 1,204.00 (25%)
Each
Add to cart
Quantity:
2 x 50 assays
Price (CNY)
3,664.00
Online Exclusive
Ends: 31-Dec-2025
4,868.00
Save 1,204.00 (25%)
Each
Add to cart
Hypoxia Green Reagent for Flow Cytometry is an antibody-independent reagent used to detect low levels of oxygen levels in live cells. The membrane-permeant probe releases rhodamine as oxygen levels decrease, resulting in a fluorogenic response. Hypoxia is a characteristic of many diseases, including cardiovascular disease and tumor-mediated immunosuppression and is critical to tumor survival and growth.

• Sensitive, fixed, end-point assay
• Detects decreases in oxygen in live cells
• Fluorogenic, non-antibody based probe

Specifications
Detection MethodFluorescence
FormatVial(s)
Quantity2 x 50 assays
Shipping ConditionRoom Temperature
Emission488
For Use With (Equipment)Flow Cytometer
Product TypeReagent
Unit SizeEach
Contents & Storage
2 vials of 50 tests each
  • Store at -5°C to -30°C.

    • Frequently asked questions (FAQs)

    Can the Hypoxia Green Reagent for Flow Cytometry be used in cells expressing fluorescent proteins?

    Yes, as long as the fluorescent protein does not emit in the green range (˜510–535 nm). For cells that do not express a fluorescent protein, we recommend viewing unstained cells under the FITC channel to examine autofluorescence. For cells expressing a fluorescent protein, we recommend analyzing unstained cell samples to determine the extent the fluorescent protein emission may overlap in the FITC channel.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Our Hypoxia Green Reagent for Flow Cytometry is already highly fluorescent before using the product. What could have caused this?

    This could be due to storage of the product under anaerobic or low oxygen conditions. The Hypoxia Green Reagent for Flow Cytometry increases in fluorescence upon exposure to low oxygen environments, and this change is not reversible. For some ROS indicators, we recommend storing the reagent under dry nitrogen or argon to prevent oxidation during storage. However, this is not appropriate for the Hypoxia Green Reagent for Flow Cytometry.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    What is different between the use of the Hypoxia Green Reagent for Flow Cytometry (Cat No. H20035) versus imaging using the Image-iT Green Hypoxia Reagent (Cat Nos. I14833, I14834)?

    The difference between these products is in the final working concentration of the reagent and incubation time. For flow cytometry applications, we recommend using a final concentration in the range of 0.5 to 1 µM with an incubation time ranging from 2 to 3 hrs. For imaging, we recommend a final concentration in the range of 1 to 10 µM with an incubation time ranging from 30 mins to 1 hr. For both applications, one should optimize the final working concentration and incubation time.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Is Hypoxia Green Reagent for Flow Cytometry (Cat No. H20035) the same reagent as the Image-iT Green Hypoxia Reagent (Cat Nos. I14833, I14834)?

    The Hypoxia Green Reagent for Flow Cytometry and the Image-iT Green Hypoxia Reagent are the same reagent, formulated differently for flow cytometry and imaging use, respectively.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.