LB Broth (Miller's modification) - FAQs

How should I prepare 1 L of Miller LB Broth media (Cat. Nos. 611875000 and H26676.xx)?

To prepare 1 L of Miller LB media:

1. Add 25 g of powder to 1 L distilled water
2. Mix by vortexing
3. Sterilize by autoclaving for 25 minutes at 121 degrees C