AuthorsValderrama R, Corpas FJ, Carreras A, Fernández-Ocaña A, Chaki M, Luque F, Gómez-Rodríguez MV, Colmenero-Varea P, Del Río LA, Barroso JB
JournalFEBS Lett
PubMed ID17240373
'Nitrosative stress has become a usual term in the physiology of nitric oxide in mammalian systems. However, in plants there is much less information on this type of stress. Using olive leaves as experimental model, the effect of salinity on the potential induction of nitrosative stress was studied. The enzymatic ... More
Separation of thiols as phenyl mercury derivatives by thin-layer chromatography. I. Azathioprine and 6-mercaptopurine metabolites.
AuthorsThapliyal RC, Maddocks JL
JournalJ Chromatogr
PubMed ID730791
A method is described for converting thiol metabolites of azathioprine and 6-mercaptopurine into phenyl mercury derivatives. Separation of these derivatives was performed by chromatography on silica thin layers and they were detected by low temperature (--196 degrees) fluorescence. The parent compounds were regenerated on the chromatogram by spraying with 2 ... More
Localization of S-nitrosothiols and assay of nitric oxide synthase and S-nitrosoglutathione reductase activity in plants.
AuthorsCorpas FJ, Carreras A, Esteban FJ, Chaki M, Valderrama R, del Río LA, Barroso JB,
JournalMethods Enzymol
PubMed ID18433647
The study of the metabolism of nitric oxide and other reactive nitrogen species (RNS) in plants has been the subject of intensive work in the last decade due to the relevance of these molecules in the physiology and biochemistry of plants. However, there are still many methodological limitations in the ... More