Brown fat determination and development from muscle precursor cells by novel action of bone morphogenetic protein 6.
AuthorsSharma A, Huard C, Vernochet C, Ziemek D, Knowlton KM, Tyminski E, Paradis T, Zhang Y, Jones JE, von Schack D, Brown CT, Milos PM, Coyle AJ, Tremblay F, Martinez RV,
Journal
PubMed ID24658703
Brown adipose tissue (BAT) plays a pivotal role in promoting energy expenditure by the virtue of uncoupling protein-1 (UCP-1) that differentiates BAT from its energy storing white adipose tissue (WAT) counterpart. The clinical implication of ... More
Approaches to protozoan drug discovery: phenotypic screening.
AuthorsSykes ML, Avery VM,
Journal
PubMed ID23927763
Determining the activity of a compound and the potential impact on a diseased state is frequently undertaken using phenotypic or target-based approaches. Phenotypic screens have the advantage of the whole organism being exposed to the compound and thus all the targets and biological pathways associated with it. Cell penetration and ... More
Effect of microgrooved surface topography on osteoblast maturation and protein adsorption.
AuthorsDe Luca AC, Zink M, Weidt A, Mayr SG, Markaki AE,
Journal
PubMed ID25630566
Microgrooved surfaces have been used extensively to influence cell contact guidance. Guiding cell growth, extracellular matrix deposition, and mineralization is important for bone implant longevity. In this study, we investigated the osteoblast response to microgrooved metallic surfaces in serum-supplemented medium. Groove spacing was comparable with the spread osteoblast size. Focal ... More
BMP signaling in astrocytes downregulates EGFR to modulate survival and maturation.
Astrocytes constitute a major cell population in the brain with a myriad of essential functions, yet we know remarkably little about the signaling pathways and mechanisms that direct astrocyte maturation. To explore the signals regulating astrocyte development, we prospectively purified and cultured immature postnatal rodent astrocytes. We identified fibroblast growth ... More
Simultaneous flow cytometric analysis of IFN-? and CD4 mRNA and protein expression kinetics in human peripheral blood mononuclear cells during activation.
AuthorsVan Hoof D, Lomas W, Hanley MB, Park E,
Journal
PubMed ID25123411
The application of fluorescently-labeled antibodies for flow cytometric identification and characterization of specific cell types within heterogeneous populations by their protein expression profile is well established. While detection of proteins is informative, concomitant transcript analysis in the same cells would provide a more complete and comprehensive view of intracellular signaling ... More
Maximum Entropy Framework for Predictive Inference of Cell Population Heterogeneity and Responses in Signaling Networks.
Authors
JournalCell Syst
PubMed ID31864963
Visualization of Arenavirus RNA Species in Individual Cells by Single-Molecule Fluorescence In Situ Hybridization Suggests a Model of Cyclical Infection and Clearance during Persistence.
Authors
JournalJ Virol
PubMed ID29643234
The circadian oscillator analysed at the single-transcript level.
Authors
JournalMol Syst Biol
PubMed ID33719202
Resistance to different anthracycline chemotherapeutics elicits distinct and actionable primary metabolic dependencies in breast cancer.
Authors
JournalElife
PubMed ID34181531
High-content imaging and structure-based predictions reveal functional differences between Niemann-Pick C1 variants.
Authors
JournalTraffic
PubMed ID32144825
Human neuromuscular junction on micro-structured microfluidic devices implemented with a custom micro electrode array (MEA).
Authors
JournalLab Chip
PubMed ID34559171
Discovery of Survivin Inhibitors Part 1: Screening the Harbor Branch Pure Compound Library.
Authors
JournalMar Drugs
PubMed ID33573152
Building predictive in vitro pulmonary toxicity assays using high-throughput imaging and artificial intelligence.
Authors
JournalArch Toxicol
PubMed ID29705884
An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein.
Authors
JournalElife
PubMed ID29638216
Abl family tyrosine kinases govern IgG extravasation in the skin in a murine pemphigus model.