Plk5, a polo box domain-only protein with specific roles in neuron differentiation and glioblastoma suppression.
Authorsde Cárcer G, Escobar B, Higuero AM, García L, Ansón A, Pérez G, Mollejo M, Manning G, Meléndez B, Abad-Rodríguez J, Malumbres M,
JournalMol Cell Biol
PubMed ID21245385
'Polo-like kinases (Plks) are characterized by the presence of a specific domain, known as the polo box (PBD), involved in protein-protein interactions. Plk1 to Plk4 are involved in centrosome biology as well as the regulation of mitosis, cytokinesis, and cell cycle checkpoints in response to genotoxic stress. We have analyzed ... More
Transfer of polyglutamine aggregates in neuronal cells occurs in tunneling nanotubes.
AuthorsCostanzo M, Abounit S, Marzo L, Danckaert A, Chamoun Z, Roux P, Zurzolo C,
Journal
PubMed ID23781027
Huntington's disease (HD) is a dominantly inherited neurodegenerative disease caused by CAG expansion in the huntingtin gene, which adds a homopolymeric tract of polyglutamine (polyQ) to the encoded protein leading to the formation of toxic aggregates. Despite rapidly accumulating evidences supporting a role for intercellular transmission of protein aggregates, little ... More
Olivocochlear suppression of outer hair cells in vivo: evidence for combined action of BK and SK2 channels throughout the cochlea.
Cholinergic inhibition of cochlear hair cells via olivocochlear (OC)-efferent feedback is mediated by Ca(2+) entry through a9-/a10-nicotinic receptors, but the nature of the K(+) channels activated by this Ca(2+) entry has been debated (Yoshida N, Hequembourg SJ, Atencio CA, Rosowski JJ, Liberman MC. J Neurophysiol 85: 84-88, 2001). A recent ... More
Acetylation modulates cellular distribution and DNA sensing ability of interferon-inducible protein IFI16.
AuthorsLi T, Diner BA, Chen J, Cristea IM,
JournalProc Natl Acad Sci U S A
PubMed ID22691496
Detection of pathogenic nucleic acids is essential for mammalian innate immunity. IFN-inducible protein IFI16 has emerged as a critical sensor for detecting pathogenic DNA, stimulating both type I IFN and proinflammatory responses. Despite being predominantly nuclear, IFI16 can unexpectedly sense pathogenic DNA in both the cytoplasm and the nucleus. However, ... More
Functional analysis of centrosomal kinase substrates in Drosophila melanogaster reveals a new function of the nuclear envelope component otefin in cell cycle progression.
AuthorsHabermann K, Mirgorodskaya E, Gobom J, Lehmann V, Müller H, Blümlein K, Deery MJ, Czogiel I, Erdmann C, Ralser M, von Kries JP, Lange BM,
JournalMol Cell Biol
PubMed ID22751930
Phosphorylation is one of the key mechanisms that regulate centrosome biogenesis, spindle assembly, and cell cycle progression. However, little is known about centrosome-specific phosphorylation sites and their functional relevance. Here, we identified phosphoproteins of intact Drosophila melanogaster centrosomes and found previously unknown phosphorylation sites in known and unexpected centrosomal components. ... More
The use of real-time cell analyzer technology in drug discovery: defining optimal cell culture conditions and assay reproducibility with different adherent cellular models.
AuthorsAtienzar FA, Tilmant K, Gerets HH, Toussaint G, Speeckaert S, Hanon E, Depelchin O, Dhalluin S,
JournalJ Biomol Screen
PubMed ID21518825
The use of impedance-based label-free technology applied to drug discovery is nowadays receiving more and more attention. Indeed, such a simple and noninvasive assay that interferes minimally with cell morphology and function allows one to perform kinetic measurements and to obtain information on proliferation, migration, cytotoxicity, and receptor-mediated signaling. The ... More
Network of mutually repressive metastasis regulators can promote cell heterogeneity and metastatic transitions.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID24395801
Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates.
Authors
JournalCell Rep
PubMed ID32375049
Mammalian CORVET is required for fusion and conversion of distinct early endosome subpopulations.
Authors
JournalTraffic
PubMed ID25266290
Large-Scale Screening and Identification of Novel Ebola Virus and Marburg Virus Entry Inhibitors.
Authors
JournalAntimicrob Agents Chemother
PubMed ID27161622
FUS inclusions disrupt RNA localization by sequestering kinesin-1 and inhibiting microtubule detyrosination.
Authors
JournalJ Cell Biol
PubMed ID28298410
Evaluation of Phenol-Substituted Diphyllin Derivatives as Selective Antagonists for Ebola Virus Entry.
Authors
JournalACS Infect Dis
PubMed ID35357134
Early spatiotemporal-specific changes in intermediate signals are predictive of cytotoxic sensitivity to TNFα and co-treatments.
Authors
JournalSci Rep
PubMed ID28272488
A Specialized Niche in the Pancreatic Microenvironment Promotes Endocrine Differentiation.
Authors
JournalDev Cell
PubMed ID32857951
RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation.
Authors
JournalCell
PubMed ID32302572
Drugs that inhibit TMEM16 proteins block SARS-CoV-2 spike-induced syncytia.