Matrix architecture dictates three-dimensional migration modes of human macrophages: differential involvement of proteases and podosome-like structures.
AuthorsVan Goethem E, Poincloux R, Gauffre F, Maridonneau-Parini I, Le Cabec V,
JournalJ Immunol
PubMed ID20018633
'Tissue infiltration of macrophages, although critical for innate immunity, is also involved in pathologies, such as chronic inflammation and cancer. In vivo, macrophages migrate mostly in a constrained three-dimensional (3D) environment. However, in vitro studies, mainly focused on two dimensions, do not provide meaningful clues about the mechanisms involved in ... More
Development of high-content imaging assays for lethal viral pathogens.
'Filoviruses such as Ebola (EBOV) and Marburg (MARV) are single-stranded negative sense RNA viruses that cause acute hemorrhagic fever with high mortality rates. Currently, there are no licensed vaccines or therapeutics to counter filovirus infections in humans. The development of higher throughput/high-content primary screening assays followed by validation using the ... More
Regulation of icam-1 in cells of the monocyte/macrophage system in microgravity.
AuthorsPaulsen K, Tauber S, Dumrese C, Bradacs G, Simmet DM, Gölz N, Hauschild S, Raig C, Engeli S, Gutewort A, Hürlimann E, Biskup J, Unverdorben F, Rieder G, Hofmänner D, Mutschler L, Krammer S, Buttron I, Philpot C, Huge A, Lier H, Barz I, Engelmann F, Layer LE, Thiel CS, Ullrich O,
Journal
PubMed ID25654110
Cells of the immune system are highly sensitive to altered gravity, and the monocyte as well as the macrophage function is proven to be impaired under microgravity conditions. In our study, we investigated the surface expression of ICAM-1 protein and expression of ICAM-1 mRNA in cells of the monocyte/macrophage system ... More
Comparison of a high-throughput high-content intracellular Leishmania donovani assay with an axenic amastigote assay.
AuthorsDe Rycker M, Hallyburton I, Thomas J, Campbell L, Wyllie S, Joshi D, Cameron S, Gilbert IH, Wyatt PG, Frearson JA, Fairlamb AH, Gray DW,
JournalAntimicrob Agents Chemother
PubMed ID23571538
Visceral leishmaniasis is a neglected tropical disease with significant health impact. The current treatments are poor, and there is an urgent need to develop new drugs. Primary screening assays used for drug discovery campaigns have typically used free-living forms of the Leishmania parasite to allow for high-throughput screening. Such screens ... More
IFITM-2 and IFITM-3 but not IFITM-1 restrict Rift Valley fever virus.
AuthorsMudhasani R, Tran JP, Retterer C, Radoshitzky SR, Kota KP, Altamura LA, Smith JM, Packard BZ, Kuhn JH, Costantino J, Garrison AR, Schmaljohn CS, Huang IC, Farzan M, Bavari S,
Journal
PubMed ID23720721
We show that interferon-induced transmembrane protein 1 (IFITM-1), IFITM-2, and IFITM-3 exhibit a broad spectrum of antiviral activity against several members of the Bunyaviridae family, including Rift Valley fever virus (RVFV), La Crosse virus, Andes virus, and Hantaan virus, all of which can cause severe disease in humans and animals. ... More
High-content phenotypic screening and triaging strategy to identify small molecules driving oligodendrocyte progenitor cell differentiation.
AuthorsPeppard JV, Rugg CA, Smicker MA, Powers E, Harnish E, Prisco J, Cirovic D, Wright PS, August PR, Chandross KJ,
Journal
PubMed ID25394729
Multiple Sclerosis is a demyelinating disease of the CNS and the primary cause of neurological disability in young adults. Loss of myelinating oligodendrocytes leads to neuronal dysfunction and death and is an important contributing factor to this disease. Endogenous oligodendrocyte precursor cells (OPCs), which on differentiation are responsible for replacing ... More
High-content phenotypic profiling of drug response signatures across distinct cancer cells.
AuthorsCaie PD, Walls RE, Ingleston-Orme A, Daya S, Houslay T, Eagle R, Roberts ME, Carragher NO,
JournalMol Cancer Ther
PubMed ID20530715
The application of high-content imaging in conjunction with multivariate clustering techniques has recently shown value in the confirmation of cellular activity and further characterization of drug mode of action following pharmacologic perturbation. However, such practical examples of phenotypic profiling of drug response published to date have largely been restricted to ... More
The Small Non-coding Vault RNA1-1 Acts as a Riboregulator of Autophagy.
Authors
JournalCell
PubMed ID30773316
SARS-CoV-2 sensing by RIG-I and MDA5 links epithelial infection to macrophage inflammation.
Authors
JournalEMBO J
PubMed ID34101213
Quantitative modelling of amino acid transport and homeostasis in mammalian cells.
Authors
JournalNat Commun
PubMed ID34489418
Phenotypic Plasticity Determines Cancer Stem Cell Therapeutic Resistance in Oral Squamous Cell Carcinoma.
Authors
JournalEBioMedicine
PubMed ID26981578
Global mapping of Salmonella enterica-host protein-protein interactions during infection.
Authors
JournalCell Host Microbe
PubMed ID34237247
Evolution of enhanced innate immune evasion by SARS-CoV-2.
Authors
JournalNature
PubMed ID34942634
Downregulation of class II phosphoinositide 3-kinase PI3K-C2β delays cell division and potentiates the effect of docetaxel on cancer cell growth.
Authors
JournalJ Exp Clin Cancer Res
PubMed ID31752944
Cell-to-cell transmission of C9orf72 poly-(Gly-Ala) triggers key features of ALS/FTD.
Authors
JournalEMBO J
PubMed ID32175624
Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166.
Authors
JournalNat Commun
PubMed ID32193381
Drugs that inhibit TMEM16 proteins block SARS-CoV-2 spike-induced syncytia.