HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit offers a complete set of reagents for performing image-based high-content screening (HCS) assays了解更多信息
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货号
数量
H34158
1200 Assays
货号 H34158
价格(CNY)
12,217.00
Each
添加至购物车
数量:
1200 Assays
价格(CNY)
12,217.00
Each
添加至购物车
HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit offers a complete set of reagents for performing image-based high-content screening (HCS) assays to characterize phospolipidosis and steatosis, toxic side effects on lipid metabolism that can be triggered by drugs or other compounds. The kit supplies LipidTOX™ Red phospholipid stain and LipidTOX™ Green neutral lipid stain, which can be used sequentially for the analysis of phospholipidosis and steatosis, respectively, or can be used in isolation for single-parameter analysis. Both fluorescent probes can be easily detected with fluorescence microscopes and can be quantified with stand-alone image analysis software or the built-in image analysis software of most HCS readers. In addition to the phospholipid and neutral lipid stains, this kit supplies the nuclear stain Hoechst 33342, propranolol and cyclosporin A (control compounds), and DMSO. Sufficient reagents are supplied for 2, 96-well plates (H34157) or 10, 96-well plates (H34158).
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型其他标记或染料
产品规格96 孔板
数量1200 Assays
运输条件室温
适用于(设备)高内涵分析仪
产品线LipidTOX™, 分子探针™
产品类型磷脂沉积和脂肪变性检测试剂盒
Unit SizeEach
内容与储存
在 -20°C 下干燥并避光储存时,该试剂盒可保持稳定至少 6 个月。
常见问题解答 (FAQ)
I'm seeing aggregates in my thawed LipidTox stain solutions. Is this okay?
After thawing LipidTOX Red phospholipidosis detection reagent, some minute aggregates might be observed in the solution. They usually will disappear if the vial is incubated in a 37 degrees C water bath for 5 minutes. These aggregates do not affect the performance of the assay.
Any aggregates that remain after the stain is diluted in media are removed by 0.2 µm filtration before the labeling solution is added to the cells, as recommended in the protocol.