Fluorescent high-content imaging allows the discrimination and quantitation of E-LDL-induced lipid droplets and Ox-LDL-generated phospholipidosis in human macrophages.
AuthorsGrandl M, Schmitz G,
JournalCytometry A
PubMed ID20014301
'Macrophage foam cells formed during uptake of atherogenic lipoproteins are a hallmark of atherosclerotic lesion development. In this study, human macrophages were incubated with two prototypic atherogenic LDL modifications enzymatically degraded LDL (E-LDL) and oxidized LDL (Ox-LDL) prepared from the same donor LDL. To detect differences in macrophage lipid storage, ... More
Effect of growth factors on the proliferation and gene expression of human meibomian gland epithelial cells.
AuthorsLiu S, Kam WR, Ding J, Hatton MP, Sullivan DA,
JournalInvest Ophthalmol Vis Sci
PubMed ID23493293
'We hypothesize that growth factors, including epidermal growth factor (EGF) and bovine pituitary extract (BPE), induce proliferation, but not differentiation (e.g., lipid accumulation), of human meibomian gland epithelial cells. We also hypothesize that these actions involve a significant upregulation of genes linked to cell cycle processes, and a significant downregulation ... More
Enhancement of BODIPY505/515 lipid fluorescence method for applications in biofuel-directed microalgae production.
AuthorsBrennan L, Blanco Fernández A, Mostaert AS, Owende P,
JournalJ Microbiol Methods
PubMed ID22521923
'This paper describes a microalgal cell lipid fluorescence enhancement method using BODIPY(505/515), which can be used to screen for lipids in wild-type microalgae and to monitor lipid content within microalgae production processes to determine optimal harvesting time. The study was based on four microalgae species (Dunaliella teteriolecta, Tetraselmis suecica, Nannochloropsis ... More
Increased lipid accumulation in the Chlamydomonas reinhardtii sta7-10 starchless isoamylase mutant and increased carbohydrate synthesis in complemented strains.
AuthorsWork VH, Radakovits R, Jinkerson RE, Meuser JE, Elliott LG, Vinyard DJ, Laurens LM, Dismukes GC, Posewitz MC,
JournalEukaryot Cell
PubMed ID20562225
'The accumulation of bioenergy carriers was assessed in two starchless mutants of Chlamydomonas reinhardtii (the sta6 [ADP-glucose pyrophosphorylase] and sta7-10 [isoamylase] mutants), a control strain (CC124), and two complemented strains of the sta7-10 mutant. The results indicate that the genetic blockage of starch synthesis in the sta6 and sta7-10 mutants ... More
SIRT1 regulates differentiation of mesenchymal stem cells by deacetylating ß-catenin.
AuthorsSimic P, Zainabadi K, Bell E, Sykes DB, Saez B, Lotinun S, Baron R, Scadden D, Schipani E, Guarente L,
JournalEMBO Mol Med
PubMed ID23364955
'Mesenchymal stem cells (MSCs) are multi-potent cells that can differentiate into osteoblasts, adipocytes, chondrocytes and myocytes. This potential declines with aging. We investigated whether the sirtuin SIRT1 had a function in MSCs by creating MSC specific SIRT1 knock-out (MSCKO) mice. Aged MSCKO mice (2.2 years old) showed defects in tissues ... More
The lysosomal transfer of LDL/cholesterol from macrophages into vascular smooth muscle cells induces their phenotypic alteration.
AuthorsWeinert S, Poitz DM, Auffermann-Gretzinger S, Eger L, Herold J, Medunjanin S, Schmeisser A, Strasser RH, Braun-Dullaeus RC,
JournalCardiovasc Res
PubMed ID23250921
'Macrophages (MPs) and vascular smooth muscle cells (VSMCs) closely interact within the growing atherosclerotic plaque. An in vitro co-culture model was established to study how MPs modulate VSMC behaviour. MPs were exposed to fluorescence-labelled-acetylated LDL (FL-acLDL) prior to co-culture with VSMCs. Fluorescence microscopy visualized first transport of FL-acLDL within 6 ... More
Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae.
AuthorsPereira H, Barreira L, Mozes A, Florindo C, Polo C, Duarte CV, Custódio L, Varela J,
JournalBiotechnol Biofuels
PubMed ID22192119
We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the ... More
Fluorescent probes for exploring plant cell wall deconstruction: a review.
AuthorsPaës G,
Journal
PubMed ID24995923
Plant biomass is a potential resource of chemicals, new materials and biofuels that could reduce our dependency on fossil carbon, thus decreasing the greenhouse effect. However, due to its chemical and structural complexity, plant biomass is recalcitrant to green biological transformation by enzymes, preventing the establishment of integrated bio-refineries. In ... More
Plasmin promotes foam cell formation by increasing macrophage catabolism of aggregated low-density lipoprotein.
AuthorsHaka AS, Grosheva I, Singh RK, Maxfield FR,
Journal
PubMed ID23702659
The plasmin/plasminogen system is involved in atherosclerosis. However, the mechanisms by which it stimulates disease are not fully defined. A key event in atherogenesis is the deposition of low-density lipoprotein (LDL) on arterial walls where it is modified, aggregated, and retained. Macrophages are recruited to clear the lipoproteins, and they ... More
Ultrastructure of a novel bacterial form located in Staphylococcus aureus in vitro and in vivo catheter-associated biofilms.
Bacterial biofilms are ubiquitous in nature, industry, and medicine, and understanding their development and cellular structure is critical in controlling the unwanted consequences of biofilm growth. Here, we report the ultrastructure of a novel bacterial form observed by scanning electron microscopy in the luminal vegetations of catheters from patients with ... More
The clock gene, brain and muscle Arnt-like 1, regulates adipogenesis via Wnt signaling pathway.
AuthorsGuo B, Chatterjee S, Li L, Kim JM, Lee J, Yechoor VK, Minze LJ, Hsueh W, Ma K,
JournalFASEB J
PubMed ID22611086
Circadian clocks in adipose tissue are known to regulate adipocyte biology. Although circadian dysregulation is associated with development of obesity, the underlying mechanism has not been established. Here we report that disruption of the clock gene, brain and muscle Arnt-like 1 (Bmal1), in mice led to increased adipogenesis, adipocyte hypertrophy, ... More
STARD4 abundance regulates sterol transport and sensing.
Nonvesicular transport of cholesterol plays an essential role in the distribution and regulation of cholesterol within cells, but it has been difficult to identify the key intracellular cholesterol transporters. The steroidogenic acute regulatory-related lipid-transfer (START) family of proteins is involved in several pathways of nonvesicular trafficking of sterols. Among them, ... More
Cancer-preventive rexinoid modulates neutral lipid contents of mammary epithelial cells through a peroxisome proliferator-activated receptor ?-dependent mechanism.
AuthorsUray IP, Rodenberg JM, Bissonnette RP, Brown PH, Mancini MA,
JournalMol Pharmacol
PubMed ID22053058
Synthetic rexinoids effectively suppress both estrogen receptor-positive and estrogen receptor-negative mammary tumors in animal models, which makes them prime candidates for a novel class of cancer-preventive agents. When used in combination with chemotherapy for non-small-cell lung cancer, the rexinoid bexarotene was most effective for patients who developed hypertriglyceridemia as a ... More
In situ analysis of heterogeneity in the lipid content of single green microalgae in alginate hydrogel microcapsules.
AuthorsLee DH, Bae CY, Han JI, Park JK,
Journal
PubMed ID24007509
Microalgae, a group of microorganisms that grow using sunlight as the sole energy source and carbon dioxide as an only carbon source, have been considered as a feedstock of choice for the production of biofuels such as biodiesel. To explore the economic feasibility of such application, however, many technical hurdles ... More
Starving neurons show sex difference in autophagy.
AuthorsDu L, Hickey RW, Bayir H, Watkins SC, Tyurin VA, Guo F, Kochanek PM, Jenkins LW, Ren J, Gibson G, Chu CT, Kagan VE, Clark RS,
JournalJ Biol Chem
PubMed ID19036730
Sex-dependent differences in adaptation to famine have long been appreciated, thought to hinge on female versus male preferences for fat versus protein sources, respectively. However, whether these differences can be reduced to neurons, independent of typical nutrient depots, such as adipose tissue, skeletal muscle, and liver, was heretofore unknown. A ... More
AuthorsYoum YH, Yang H, Sun Y, Smith RG, Manley NR, Vandanmagsar B, Dixit VD,
JournalJ Biol Chem
PubMed ID19054770
With progressive aging, adipocytes are the major cell types that constitute the bulk of thymic microenvironment. Understanding the origin of thymic adipocytes and mechanisms responsible for age-related thymic adiposity is thus germane for the design of long lasting thymic rejuvenation strategies. We have recently identified that ghrelin, an orexigenic anti-inflammatory ... More
Adipocyte Piezo1 mediates obesogenic adipogenesis through the FGF1/FGFR1 signaling pathway in mice.
Authors
JournalNat Commun
PubMed ID32385276
Generation of Human Fatty Livers Using Custom-Engineered Induced Pluripotent Stem Cells with Modifiable SIRT1 Metabolism.
Authors
JournalCell Metab
PubMed ID31390551
The Adipose Tissue Microenvironment Regulates Depot-Specific Adipogenesis in Obesity.
Authors
JournalCell Metab
PubMed ID27320063
RSPO3 impacts body fat distribution and regulates adipose cell biology in vitro.
Authors
JournalNat Commun
PubMed ID32493999
Stat3 controls cell death during mammary gland involution by regulating uptake of milk fat globules and lysosomal membrane permeabilization.
Authors
JournalNat Cell Biol
PubMed ID25283994
Interplay and cooperation between SREBF1 and master transcription factors regulate lipid metabolism and tumor-promoting pathways in squamous cancer.
Authors
JournalNat Commun
PubMed ID34272396
Human beige adipocytes for drug discovery and cell therapy in metabolic diseases.
Authors
JournalNat Commun
PubMed ID32488069
Brown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis.
Authors
JournalNat Commun
PubMed ID33772024
Omega-3 Fatty Acids Activate Ciliary FFAR4 to Control Adipogenesis.
Authors
JournalCell
PubMed ID31761534
Rapid depot-specific activation of adipocyte precursor cells at the onset of obesity.