Fusogenic properties of the C-terminal domain of the Alzheimer beta-amyloid peptide.
AuthorsPillot T,Goethals M,Vanloo B,Talussot C,Brasseur R,Vandekerckhove J,Rosseneu M,Lins L
JournalThe Journal of biological chemistry
PubMed ID8910517
A series of natural peptides and mutants, derived from the Alzheimer beta-amyloid peptide, was synthesized, and the potential of these peptides to induce fusion of unilamellar lipid vesicles was investigated. These peptide domains were identified by computer modeling and correspond to respectively the C-terminal (e.g. residues 29-40 and 29-42) and ... More
Mapping the interfacial binding surface of human secretory group IIa phospholipase A2.
AuthorsSnitko Y, Koduri RS, Han SK, Othman R, Baker SF, Molini BJ, Wilton DC, Gelb MH, Cho W
JournalBiochemistry
PubMed ID9398150
Human secretory group IIa phospholipase A2 (hIIa-PLA2) contains a large number of prominent cationic patches on its molecular surface and has exceptionally high affinity for anionic surfaces, including anionic membranes. To identify the cationic amino acid residues that support binding of hIIa-PLA2 to anionic membranes, we have performed extensive site-directed ... More
Lipid binding to sterol carrier protein-2 is inhibited by ethanol.
AuthorsAvdulov NA, Chochina SV, Igbavboa U, Warden CS, Schroeder F, Wood WG
JournalBiochim Biophys Acta
PubMed ID9931423
'Sterol carrier protein-2 (SCP-2) is an intracellular lipid carrier protein that binds cholesterol, phospholipids, fatty acids and other ligands. It has been reported that expression of SCP-2 was increased in brain nerve endings or synaptosomes of chronic ethanol-treated mice and it was shown that cholesterol homeostasis was altered in brain ... More
Reversibility of the binding of cytochrome c to liposomes. Implications for lipid-protein interactions.
AuthorsRytömaa M, Kinnunen PK
JournalJ Biol Chem
PubMed ID7852404
'Studies on the membrane binding of cytochrome c revealed liposome-associated and soluble cytochrome c not to be in rapid equilibrium. In brief, cytochrome c attached to pyrene phospholipid-labeled, fluorescent liposomes containing either 17.6 mol % cardiolipin (CL) or 30 mol % egg phosphatidylglycerol (PG) is practically not at all or ... More
Detection of membrane packing defects by time-resolved fluorescence depolarization.
AuthorsChen SY, Cheng KH
JournalBiophys J
PubMed ID8842226
'Packing defects in lipid bilayer play a significant role in the biological activities of cell membranes. Time-resolved fluorescence depolarization has been used to detect and characterize the onset of packing defects in binary mixtures of dilinoleoylphosphatidylethanolamine/1-palmitoyl-2- oleoylphosphatidylcholine (PE/PC). These PE/PC mixtures exhibit mesoscopic packing defect state (D), as well as ... More
Molecular and macromolecular specificity of human plasma phospholipid transfer protein.
AuthorsRao R, Albers JJ, Wolfbauer G, Pownall HJ
JournalBiochemistry
PubMed ID9132017
'Phospholipid transfer protein (PLTP), also known as lipid transfer protein 2 (LTP-2), mediates a transfer of phospholipids between high-density lipoproteins (HDL). The molecular and macromolecular specificities of recombinant human PLTP were studied using a fluorometric assay based on the excimer fluorescence of pyrenyl lipids. To determine lipoprotein specificity of PLTP, ... More
Lipid structural reorganization induced by the pancreatic lipase cofactor, procolipase.
AuthorsMomsen WE, Momsen MM, Brockman HL
JournalBiochemistry
PubMed ID7766639
'Pancreatic colipase and its precursor, procolipase, facilitate interfacial lipid hydrolysis catalyzed by pancreatic lipase. To better understand how procolipase functions, its interactions with mixed-lipid monolayers at the argon-buffer interface have been characterized. The lipid mixtures consisted of 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine and either 1,3-dioleoylglycerol, a model lipase substrate, or 13,16-cis,cis-docosadienoic acid, a model ... More
Excimer-forming lipids in membrane research.
AuthorsGalla HJ, Hartmann W
JournalChem Phys Lipids
PubMed ID7418114
'Pyrenedecanoic acid and pyrene lecithin are optical probes well suited to investigate lipid bilayer membranes. The method is based on the determination of the formation of excited dimers or excimers. The rate of excimer formation yields information on the dynamic molecular properties of artificial as well as of natural membranes. ... More
Mechanism of the spontaneous transfer of phospholipids between bilayers.
AuthorsRoseman MA, Thompson TE
JournalBiochemistry
PubMed ID6892607
'A fluorescent phospholipid, 1-palmitoyl-2-pyrenedecanoylphosphatidylcholine, was used to study the mechanism of spontaneous phospholipid transfer between single-walled phospholipid vesicles. The half-time for transfer of this molecule between vesicles of dimyristoylphosphatidylcholine at 36 degrees C is 13 h if flip-flop is negligible or 24 h if flip-flop is faster than intervesicle exchange. ... More
Lipid binding to amyloid beta-peptide aggregates: preferential binding of cholesterol as compared with phosphatidylcholine and fatty acids.
AuthorsAvdulov NA, Chochina SV, Igbavboa U, Warden CS, Vassiliev AV, Wood WG
JournalJ Neurochem
PubMed ID9326304
'Amyloid beta-peptide (A beta) aggregates are one of the key neuropathological characteristics of Alzheimer''s disease. A beta belongs to a group of proteins that aggregate and form beta-sheets, and some of these proteins bind cholesterol and other lipids. The purpose of the experiments reported here was to determine if cholesterol, ... More
The lateral distribution of pyrene-labeled sphingomyelin and glucosylceramide in phosphatidylcholine bilayers.
AuthorsHresko RC, Sugár IP, Barenholz Y, Thompson TE
JournalBiophys J
PubMed ID3593870
'The lateral distribution of N-[10(1-pyrenyl)decanoyl]-sphingomyelin (PyrSPM) and N-[10(1-pyrenyl)decanoyl]-glucocerebroside (PyrGlcCer) was studied in multilamellar vesicles of 1,2-dipalmitoyl-, 1,2-dimyristoyl-, and 1-palmitoyl-2-oleoyl-phosphatidylcholine (DPPC, DMPC, and POPC, respectively) under anaerobic conditions by determining the excimer-to-monomer fluorescence intensity ratio (E/M) as a function of temperature. The E/M(T) curves for PyrSPM and PyrGlcCer in the three ... More
Use of polymerized mixed liposomes to study interactions of phospholipase A2 with membranes.
AuthorsWu SK, Cho W
JournalBiochemistry
PubMed ID8268165
'Polymerized liposomes of thiol-based phospholipids, 1,2-bis[12-(lipoyloxy)dodecanoyl]-sn-glycero-3-phosphocholine (BLPC) and -phosphoglycerol (BLPG) were used to study interactions of several phospholipases A2 (PLA2) with membranes. Large liposomes (an average diameter of 100 +/- 10 nm) prepared from BLPC or BLPG were readily hydrolyzed by PLA2. Once polymerized, however, these liposomes were resistant to ... More
Changes in the lipid dynamics of liposomal membranes induced by poly(ethylene glycol): free volume alterations revealed by inter- and intramolecular excimer-forming phospholipid analogs.
AuthorsLehtonen JY, Kinnunen PK
JournalBiophys J
PubMed ID8075332
'Influence of osmotic shrinkage, swelling, and dehydration on large unilamellar liposomes (LUVs) of 1,2-dioleoylsn-glycero-3-phosphocholine (DOPC) was investigated using the fluorescent lipid probes 1-palmitoyl-2-[10-(pyren-1-yl)]-decanoyl-sn-glycero-3-phosphocholi ne (PPDPC) and 1,2-bis[10-(pyren-1-yl)]decanoyl-sn-glycero-3-phosphocholine (bisPDPC). Increasing concentrations of poly(ethylene glycol) (PEG, average molecular weight of 6000) producing osmotic gradients delta omega up to 250 mOsm/kg were first ... More
Fourier transform infrared study on the thermotropic behaviour of fully hydrated 1-palmitoyl-2-[10-(pyren-1-y) decanoyl]-sn-glycero-3-phosphatidylcholine.
AuthorsLotta TI, Virtanen JA, Kinnunen PK
JournalChem Phys Lipids
PubMed ID3338098
'Fourier transform infrared (FTIR) spectroscopy was used to study the thermotropic behaviour of fully hydrated 1-palmitoyl-2-[10-(pyren-1-yl)-decanoyl]-sn-glycero-3-phosphatidyl choline (PPDPC) in the temperature range of 3-30 degrees C. Several changes in the spectral features of PPDPC were observed. Major alterations analogous to the gel-to-liquid crystalline phase transition of saturated phosphatidylcholines were evident ... More
Transfer of phosphatidylcholine, phosphatidylethanolamine and sphingomyelin from low- and high-density lipoprotein to human platelets.
AuthorsEngelmann B, Kögl C, Kulschar R, Schaipp B
JournalBiochem J
PubMed ID8645158
'Following a 1 h incubation of human platelets with low-density lipoprotein (LDL) labelled in the apoprotein fraction (125I-apoB) or in phospholipid fractions [14C-labelled phosphatidylcholine (PC), phosphatidylethanolamine (PE) or sphingomyelin (SM)], the percentage of total 14C associated with the cells was about 3-fold higher than the percentage of 125I. Differences in ... More
Sphingomyelinase induces lipid microdomain formation in a fluid phosphatidylcholine/sphingomyelin membrane.
AuthorsHolopainen JM, Subramanian M, Kinnunen PK
JournalBiochemistry
PubMed ID9860872
'The behaviors of two chemically well-defined sphingolipids, N-palmitoyl-sphingomyelin (C16:0-SM) and the corresponding ceramide (C16:0-Cer), in a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) matrix were compared. Minor attenuation of lateral diffusion upon increasing the mole fraction of C16:0-SM (XSM, up to 0.25) was indicated by the slight decrement in the excimer/monomer intensity ratio (Ie/Im) for ... More
Crotoxin, a phospholipase A2 neurotoxin from the South American rattlesnake Crotalus durissus terrificus: purification of several isoforms and comparison of their molecular structure and of their biological activities.
AuthorsFaure G, Bon C
JournalBiochemistry
PubMed ID3349062
'Crotoxin, the major toxin of the venom of the South American rattlesnake Crotalus durissus terrificus is a mixture of several isoforms that differ slightly in their molecular structure. The toxin consists of two nonidentical subunits: a basic and weakly toxic phospholipase A2, component B, and an acidic and nontoxic subunit, ... More
Molecular mechanism of lateral diffusion of py(10)-PC and free pyrene in fluid DMPC bilayers.
AuthorsMartins J, Melo E
JournalBiophys J
PubMed ID11159450
'From the study of the kinetics of the fluorescence self-quenching of pyrene in multilamellar vesicles of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) in the fluid phase we obtain the molecular diffusion parameters, diffusion coefficients, and activation energies for lateral diffusion for three probes: 1-palmitoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-phosphocholine (py(10)-PC), pyrene, and 1-pyrenebutanoic acid (py-but). The experiments are done ... More
Novel Fusogenic Liposomes for Fluorescent Cell Labeling and Membrane Modification.
AuthorsCsisza´r A, Hersch N, Dieluweit S, Biehl R, Merkel R, Hoffmann B,
JournalBioconjug Chem
PubMed ID20184308
'Efficient delivery of biomolecules into membranes of living cells as well as cell surface modifications are major biotechnological challenges. Here, novel liposome systems based on neutral and cationic lipids in combination with lipids modified by aromatic groups are introduced for such applications. The fusion efficiency of these liposome systems was ... More
Membrane perturbation and the mechanism of lipid-mediated transfer of DNA into cells.
AuthorsMui B, Ahkong QF, Chow L, Hope MJ
JournalBiochim Biophys Acta
PubMed ID11030588
'Mixtures of cationic lipids and unsaturated phosphatidylethanolamine are used extensively for the intracellular delivery of plasmids and antisense oligodeoxynucleotides (ODN) in vitro. However, the mechanism by which cytoplasmic delivery of these large molecules is achieved remains unclear. The common hypothesis is that phosphatidylethanolamine promotes fusion of lipid/DNA particles with endosomal ... More
Biophysical and structural properties of DNA.diC(14)-amidine complexes. Influence of the DNA/lipid ratio.
AuthorsPector V, Backmann J, Maes D, Vandenbranden M, Ruysschaert JM
JournalJ Biol Chem
PubMed ID10896680
'Cationic liposomes are used as vectors for gene delivery both in vitro and in vivo. Comprehension of both DNA/liposome interactions on a molecular level and a description of structural modifications involved, are prerequisites to an optimization of the transfection protocol and, thus, successful application in therapy. Formation and stability of ... More
Protein-catalyzed transport of ether phospholipids.
AuthorsSzolderits G, Daum G, Paltauf F, Hermetter A
JournalBiochim Biophys Acta
PubMed ID2012818
'The protein-catalyzed transfer of alkenylacyl-, alkylacyl-, or diacyl-glycerophosphocholines, carrying a pyrenedecanoyl residue as a fluorogenic acyl chain, was studied using unilamellar bilayer vesicles as donor and acceptor membranes in a fluorescence assay. Specific phospholipid transfer proteins, such as phosphatidylinositol transfer protein from yeast and phosphatidylcholine transfer protein from bovine liver ... More
Fluorescence studies of phosphatidylcholine micelle mixing: relevance to phospholipase kinetics.
AuthorsSoltys CE, Roberts MF
JournalBiochemistry
PubMed ID7918374
'Two fluorescent micellar phospholipid probes (1-hexanoyl-2-(1-pyrenebutyroyl)phosphatidylcholine and 1-octanoyl-2-(1-pyrenebutyroyl)phosphatidylcholine) have been synthesized, characterized, and used to monitor the dynamics of lipid/amphiphile exchange in a variety of detergents and phospholipid micelles using both steady-state and stopped-flow fluorescence techniques. The ratio of the pyrene monomer to excimer band is a good indicator of ... More
Effects of temperature and lipid composition on the serum albumin-induced aggregation and fusion of small unilamellar vesicles.
AuthorsSchenkman S, Araujo PS, Dijkman R, Quina FH, Chaimovich H
JournalBiochim Biophys Acta
PubMed ID7317422
'Small unilamellar vesicles of egg phosphatidylcholine (PC) or dimyristoylphosphatidylcholine, mixed with small unilamellar vesicles labelled with 2-(10-(1-pyrene)decanoyl)phosphatidylcholine, exhibit a constant average size and excimer to monomer (E/M) ratio for several hours when incubated at pH 3.6 at a temperature higher than the phase transition temperature (Tc) of the lipids. Addition ... More
Pyrene-labeled gangliosides: micelle formation in aqueous solution, lateral diffusion, and thermotropic behavior in phosphatidylcholine bilayers.
AuthorsOllmann M, Schwarzmann G, Sandhoff K, Galla HJ
JournalBiochemistry
PubMed ID3676298
'By use of the excimer technique, the formation in aqueous solution of pyrene-labeled ganglioside micelles and their lateral diffusion and distribution in phosphatidylcholine membranes were investigated. For these studies 12-(1-pyrenyl)dodecanoic acid was covalently attached to the ceramide part of lysogangliosides GM1, GM2, GM3, GD1a, and GD1b. The 12-(1-pyrenyl)dodecanoic acid substitute ... More
Lipid-modified, cysteinyl-containing peptides of diverse structures are efficiently S-acylated at the plasma membrane of mammalian cells.
AuthorsSchroeder H, Leventis R, Shahinian S, Walton PA, Silvius JR
JournalJ Cell Biol
PubMed ID8707845
'A variety of cysteine-containing, lipid-modified peptides are found to be S-acylated by cultured mammalian cells. The acylation reaction is highly specific for cysteinyl over serinyl residues and for lipid-modified peptides over hydrophilic peptides. The S-acylation process appears by various criteria to be enzymatic and resembles the S-acylation of plasma membrane-associated ... More
Spontaneous phosphatidylcholine transfer by collision between vesicles at high lipid concentration.
AuthorsJones JD, Thompson TE
JournalBiochemistry
PubMed ID2640559
'The transfer kinetics of [3H]-1-palmitoyl-2-oleoylphosphatidylcholine ([3H]POPC) and 1-palmitoyl-2-(pyrenyldecanoyl)phosphatidylcholine (PyrPC) from POPC small unilamellar vesicles were examined at 37 degrees C with lipid concentrations ranging from 0.1 to 40 mM. The rate of [3H]POPC transfer was determined by analyzing the movement of this lipid from charged donor to neutral acceptor vesicles. ... More
Membrane fusion of Semliki Forest virus in a model system: correlation between fusion kinetics and structural changes in the envelope glycoprotein.
AuthorsBron R, Wahlberg JM, Garoff H, Wilschut J
JournalEMBO J
PubMed ID8440260
'This paper presents a kinetic analysis of low-pH-induced fusion of Semliki Forest virus (SFV) with cholesterol-containing unilamellar lipid vesicles (liposomes), consisting otherwise of phosphatidylcholine, phosphatidylethanolamine and sphingomyelin. Fusion is monitored continuously with a lipid mixing assay, involving virus bio-synthetically labeled with the fluorophore pyrene. At pH 5.55, 37 degrees C, ... More
Thermal stability of the plasma membrane calcium pump. Quantitative analysis of its dependence on lipid-protein interactions.
'Thermal stability of plasma membrane Ca(2+) pump was systematically studied in three micellar systems of different composition, and related with the interactions amphiphile-protein measured by fluorescence resonance energy transfer. Thermal denaturation was characterized as an irreversible process that is well described by a first order kinetic with an activation energy ... More
Lateral diffusion of ganglioside GM1 in phospholipid bilayer membranes.
AuthorsGoins B, Masserini M, Barisas BG, Freire E
JournalBiophys J
PubMed ID3755064
'The lateral diffusion coefficient of ganglioside GM1 incorporated into preformed dimyristoylphosphatidylcholine (DMPC) vesicles has been investigated under a variety of conditions using the technique of fluorescence photobleaching recovery. For these studies the fluorescent probe 5-(((2-Carbohydrazino)methyl)thio)acetyl) amino eosin was covalently attached to the periodate-oxidized sialic acid residue of ganglioside GM1. This ... More
Characteristics of the binding of tacrine to acidic phospholipids.
AuthorsLehtonen JY, Rytömaa M, Kinnunen PK
JournalBiophys J
PubMed ID9172742
'Tacrine (1,2,3,4-tetrahydro-9-acridinamine monohydrate) is an inhibitor of acetylcholinesterase currently used in the treatment of the symptoms of Alzheimer's disease. The present study demonstrates preferential binding of this drug to acidic phospholipids, as revealed by fluorescence polarization, penetration into lipid monolayers, and effects on the thermal phase behavior of dimyristoyl phosphatidic ... More
Quenching of fluorescence of pyrene-substituted lecithin by tetracyanoquinodimethane in liposomes.
AuthorsLemmetyinen H, Yliperttula M, Mikkola J, Kinnunen P
JournalBiophys J
PubMed ID2720079
'In this work we have applied a kinetic scheme derived from fluorescence kinetics of pyrene-labeled phosphatidylcholine in phosphatidylcholine membrane to explain the fluorescence quenching of 1-palmitoyl-2-(10-[pyrenl-yl]-sn-glycerol-3-phosphatidylchol ine (PPDPC) liposomes by tetracyanoquinodimethane (TCNQ). The scheme was also found to be applicable to neat PPDPC and the effect of the quencher could ... More
Regulation mechanism of the lateral diffusion of band 3 in erythrocyte membranes by the membrane skeleton.
AuthorsTomishige M, Sako Y, Kusumi A
JournalJ Cell Biol
PubMed ID9722611
'Mechanisms that regulate the movement of a membrane spanning protein band 3 in erythrocyte ghosts were investigated at the level of a single or small groups of molecules using single particle tracking with an enhanced time resolution (0.22 ms). Two-thirds of band 3 undergo macroscopic diffusion: a band 3 molecule ... More
Solution structure of Ace-AMP1, a potent antimicrobial protein extracted from onion seeds. Structural analogies with plant nonspecific lipid transfer proteins.
AuthorsTassin S, Broekaert WF, Marion D, Acland DP, Ptak M, Vovelle F, Sodano P
JournalBiochemistry
PubMed ID9521681
'The three-dimensional solution structure of Ace-AMP1, an antifungal protein extracted from onion seeds, was determined using 1H NMR spectroscopy and molecular modeling. This cationic protein contains 93 amino acid residues and four disulfide bridges. Its structure was determined from 1260 NOE-derived distance restraints and 173 dihedral restraints derived from NOEs ... More
Binding of phosphatidic acid to the protein-tyrosine phosphatase SHP-1 as a basis for activity modulation.
AuthorsFrank C, Keilhack H, Opitz F, Zschörnig O, Böhmer FD
JournalBiochemistry
PubMed ID10508402
'Activation of the SH2 domain-possessing protein-tyrosine phosphatase SHP-1 by acidic phospholipids as phosphatidic acid (PA) has been described earlier and suggested to participate in regulation of SHP-1 activity toward cellular substrates. The mechanism of this activation is poorly understood. Direct binding of phosphatidic acid to recombinant SHP-1 could be demonstrated ... More
Transfer of cholesterol and oxysterol derivatives by the nonspecific lipid transfer protein (sterol carrier protein 2): a study on its mode of action.
Authorsvan Amerongen A, Demel RA, Westerman J, Wirtz KW
JournalBiochim Biophys Acta
PubMed ID2742872
'The nonspecific lipid transfer protein (nsLTP) facilitates the transfer of both phospholipids and cholesterol between membrane interfaces. In this study, we have investigated the transport of 14C-labelled cholesterol, 7-ketocholesterol, 7 alpha-hydroxycholesterol and 25-hydroxycholesterol from a mixed lipid monolayer at the air/water interface to acceptor vesicles in the subphase. In the ... More
Effect of free fatty acids on the permeability of 1,2-dimyristoyl-sn-glycero-3-phosphocholine bilayer at the main phase transition.
AuthorsLangner M, Hui S
JournalBiochim Biophys Acta
PubMed ID10675520
'We measured the influence of saturated and unsaturated free fatty acids on the permeability and partition of ions into 1, 2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) bilayers. The bilayer permeability was measured using the depletion of N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1, 2-dihexadecanoyl-sn-glycero-3-phosphatidylethanolamine (N-NBD-PE) fluorescence as a result of its reduction by dithionite. We observed a distinct increase of ... More
Characteristics of pyrene phospholipid/gamma-cyclodextrin complex.
AuthorsTanhuanpää K, Cheng KH, Anttonen K, Virtanen JA, Somerharju P
JournalBiophys J
PubMed ID11509363
'Recently, it was demonstrated that gamma-cyclodextrins (gamma-CDs) greatly accelerates transfer of hydrophobic pyrene-labeled and other fluorescent phospholipid derivatives from vesicles to cells in culture (). To understand better the characteristics of this process, we studied the interaction of gamma-CD with pyrene-labeled phosphatidylcholines (PyrPCs) using a variety of physical methods. Either ... More
Roles of surface hydrophobic residues in the interfacial catalysis of bovine pancreatic phospholipase A2.
AuthorsLee BI, Yoon ET, Cho W
JournalBiochemistry
PubMed ID8672459
'The interfacial binding is a unique and important step in the phospholipase A2 (PLA2) catalyzed hydrolysis of phospholipids which is distinct from the binding of a substrate to the active site. To assess the roles of surface hydrophobic residues of PLA2 in these processes, we selectively mutated Leu-19 and Leu-20 ... More
Determination of the acyl chain specificity of the bovine liver phosphatidylcholine transfer protein. Application of pyrene-labeled phosphatidylcholine species.
AuthorsSomerharju PJ, van Loon D, Wirtz KW
JournalBiochemistry
PubMed ID3427069
'The phosphatidylcholine transfer protein from bovine liver has specific binding sites for the sn-1 and sn-2 acyl chains of the phosphatidylcholine molecule [Berkhout, T.A., Visser, A.J. W.G., & Wirtz, K.W.A. (1984) Biochemistry 23, 1505-1513]. In the present study, we have investigated the properties of these binding sites by determining both ... More
Membrane fusion induced by vesicular stomatitis virus depends on histidine protonation.
AuthorsCarneiro FA, Stauffer F, Lima CS, Juliano MA, Juliano L, Da Poian AT
JournalJ Biol Chem
PubMed ID12571240
'Entry of enveloped animal viruses into their host cells always depends on a step of membrane fusion triggered by conformational changes in viral envelope glycoproteins. Vesicular stomatitis virus (VSV) infection is mediated by virus spike glycoprotein G, which induces membrane fusion at the acidic environment of the endosomal compartment. VSV-induced ... More
Binding of endostatin to phosphatidylserine-containing membranes and formation of amyloid-like fibers.
AuthorsZhao H, Jutila A, Nurminen T, Wickström SA, Keski-Oja J, Kinnunen PK
JournalBiochemistry
PubMed ID15723529
'Endostatin, the 20-kDa C-terminal NC1 domain of collagen XVIII, is an endogenous inhibitor of tumor angiogenesis and tumor growth. A major problem in reconciling the many reported in vitro effects of endostatin is the lack of a high-affinity receptor, and a search for the latter continues. In accordance with the ... More
Conformation of phosphatidylcholine in neat and cholesterol-containing liquid-crystalline bilayers. Application of a novel method.
AuthorsEklund KK, Virtanen JA, Kinnunen PK, Kasurinen J, Somerharju PJ
JournalBiochemistry
PubMed ID1390642
'The conformation of phosphatidylcholine in liquid-crystalline bilayers was studied with a novel, high-resolution method employing phosphatidylcholine species containing pyrenyl moieties in both acyl chains of variable length. Analysis of the intramolecular pyrene-pyrene collision data obtained for 30 such species in terms of a simple geometrical model showed that the sn-1 ... More
Calcium-dependent annexin V binding to phospholipids: stoichiometry, specificity, and the role of negative charge.
AuthorsMeers P, Mealy T
JournalBiochemistry
PubMed ID8218240
'Annexin V is a Ca(2+)-dependent, phospholipid-binding protein that may have one or more membrane-related functions. The binding of annexin V to phospholipids in a detergent micelle matrix was studied to attempt to determine directly the stoichiometry of specific phospholipid-binding sites and the importance of negative charge. When annexin V binds ... More
Oxidation of unsaturated phospholipids in membrane bilayer mixtures is accompanied by membrane fluidity changes.
AuthorsBorst JW, Visser NV, Kouptsova O, Visser AJ
JournalBiochim Biophys Acta
PubMed ID10962288
'Steady-state and time-resolved fluorescence spectroscopy has been used to obtain information on oxidation processes and associated dynamical and structural changes in model membrane bilayers made from single unilamellar vesicles (SUV''s) of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) mixed with increasing amounts of 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (SAPC). The highly unsaturated arachidonoyl chain containing four double bonds is ... More
Fluorescence assay of the specificity of human plasma and bovine liver phospholipid transfer proteins.
AuthorsMassey JB, Hickson-Bick D, Via DP, Gotto AM, Pownall HJ
JournalBiochim Biophys Acta
PubMed ID4005271
'The specificities of a human plasma and bovine liver phospholipid transfer protein were studied using a fluorescence assay based on the transfer of pyrenyl phospholipids. This method was used previously to determine the mechanism of spontaneous transfer of phospholipids between model lipoproteins (Massey, J.B., Gotto, A.M., Jr. and Pownall, H.J. ... More
Iodide penetration into lipid bilayers as a probe of membrane lipid organization.
AuthorsLangner M, Hui SW
JournalChem Phys Lipids
PubMed ID1814637
'The quenching efficiency of iodide as a penetrating fluorescence quencher for a membrane-associated fluorophore was utilized to measure the molecular packing of lipid bilayers. The KI quenching efficiency of tryptophan-fluorescence from melittin incorporated in DMPC bilayer vesicles peaks at the phase transition temperature (24 degrees C) of DMPC, whereas acrylamide ... More
Importance of phospholipid in the folding and conformation of phosphatidylinositol transfer protein: comparison of apo and holo species.
'The significance of noncovalently bound phospholipid as a structural component of phosphatidylinositol transfer protein (PITP) and its role in acquisition and maintenance of the native conformation of the protein have been addressed by studying the refolding of PITP after exposure to 6 M guanidinium chloride (GdnCl). Protein conformations were characterized ... More
'Nitric oxide (NO) signaling is inextricably linked to both its physical and chemical properties. Due to its preferentially hydrophobic solubility, NO molecules tend to partition from the aqueous milieu into biological membranes. We hypothesized that plasma membrane ordering provided by cholesterol further couples the physics of NO diffusion with cellular ... More
Retinoid specificity of interphotoreceptor retinoid-binding protein.
AuthorsChen Y, Noy N
JournalBiochemistry
PubMed ID8075067
'Interphotoreceptor retinoid-binding protein (IRBP), the predominant protein in the interphotoreceptor matrix of retina, has been implicated in transfer of retinoids between retinal pigment epithelium and photoreceptor cells. In this work, the interactions of several retinoids with IRBP were studied in order to clarify whether the protein displays specificity toward particular ... More
Activation of murine lymphocytes by lipopolysaccharide incorporated in fusogenic, reconstituted influenza virus envelopes (virosomes).
AuthorsDijkstra J, Bron R, Wilschut J, de Haan A, Ryan JL
JournalJ Immunol
PubMed ID8757606
'We have studied the in vitro activation of murine lymphocytes with LPS incorporated in the membranes of both phospholipid vesicles (liposomes) and vesicles composed of fusogenic, reconstituted influenza virus envelopes (virosomes). The incorporation of Salmonella minnesota rough-LPS in liposomes reduced the potency of LPS to stimulate splenocyte proliferation and cell ... More
Functional signal peptide reduces bilayer thickness of phosphatidylcholine liposomes.
AuthorsTahara Y, Murata M, Ohnishi S, Fujiyoshi Y, Kikuchi M, Yamamoto Y
JournalBiochemistry
PubMed ID1390661
'To investigate the interaction between a signal peptide and the lipid bilayer, two kinds of peptides, L8-M5 (L8 = MRL8PLAALG, M5 = KVFER) and L14-M5 (L14 = MRL14PLAALG), were examined in membranes composed of dioleoylphosphatidylcholine (DOPC). Peptides L8 and L14 are artificially designed signal sequences, and M5 is the N-terminal ... More
Selective killing of T lymphocytes by phototoxic liposomes.
AuthorsYemul S, Berger C, Estabrook A, Suarez S, Edelson R, Bayley H
JournalProc Natl Acad Sci U S A
PubMed ID3491992
'Two-fold specificity in drug delivery obtained through the localized activation of drugs by physical means and the attachment of drugs to proteins that bind to target cells might be used for highly selective cancer chemotherapy or for immunosuppression. Toward this end, a monoclonal antibody against an antigen on the surface ... More
Parinaroyl and pyrenyl phospholipids as probes for the lipid surface layer of human low density lipoproteins.
AuthorsVauhkonen M, Somerharju P
JournalBiochim Biophys Acta
PubMed ID2765542
'A simple protocol employing lipid transfer proteins was developed to label human low density lipoprotein (LDL) in a controlled manner with parinaroyl and pyrenyl phosphatidylcholines. In order to study the lipid fluidity in the surface lipid layer of LDL, the temperature-dependence of both polarization (parinaroyl probes) and excimer to monomer ... More
Minor effects of bulk viscosity on lipid translational diffusion measured by the excimer formation technique.
AuthorsOllmann M, Robitzki A, Schwarzmann G, Galla HJ
JournalEur Biophys J
PubMed ID3208708
'We have investigated the effect of bulk viscosity on lipid translational diffusion using the excimer formation technique. In contrast to a study by Vaz et al. (1987), performed with the fluorescence recovery after photobleaching technique, we observed only a minor decrease of less than a factor of two for pyrene ... More
Energy transfer measurements of fusion between Sendai virus and vesicles corrected for decreased absorption of acceptor probe.
AuthorsMacDonald RI
JournalJ Biol Chem
PubMed ID3038876
'The fusion of Sendai virus at pH 4-7 with artificial lipid vesicles composed of phosphatidylserine or phosphatidylcholine was quantified by measuring fluorescence energy transfer from N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-phosphatidylethanolamine to N-(lissamine-rhodamine-B-sulfonyl)-phosphatidylethanolamine in the target membranes. About 60% of the phosphatidylserine vesicles and virus appeared to fuse at pH 4 and about 100% at ... More
Pyrenedecanoic acid and pyrene lecithin.
AuthorsGalla HJ, Hartmann W
JournalMethods Enzymol
PubMed ID7311843
Lipid interaction of tetanus neurotoxin. A calorimetric and fluorescence spectroscopy study.
AuthorsCalappi E, Masserini M, Schiavo G, Montecucco C, Tettamanti G
JournalFEBS Lett
PubMed ID1505672
The interaction of Tetanus toxin with phospholipid vesicles containing gangliosides (GD1a, GD1b or GT1b) or phosphatidic acid has been investigated at neutral or acidic pH. Change in the thermotropic properties of the vesicles occurred only after addition of the toxin at acidic pH, and led to surface binding or membrane ... More
A sphingomyelin-transferring protein from chicken liver. Use of pyrene-labeled phospholipid.
A phospholipid transfer protein was purified from chicken liver which, in addition to phosphatidylinositol (PI) and phosphatidylcholine (PC), carries sphingomyelin (SM) between membranes. For comparison, the PI-transfer protein from chicken liver only carries PI and PC. Specificity was established by use of phospholipids that carry a pyrene-labeled acyl chain. Based ... More
Mechanism of spontaneous, concentration-dependent phospholipid transfer between bilayers.
AuthorsJones JD, Thompson TE
JournalBiochemistry
PubMed ID2334718
We have previously demonstrated that spontaneous phospholipid transfer between bilayer vesicles at higher vesicle concentrations is characterized not only by a first-order desorption rate but also by a second-order process dependent on vesicle concentration (Jones & Thompson, 1989b). We have extended our studies to examine the mechanism of this second-order ... More
Unusual pressure dependence of the lateral motion of pyrene-labeled phosphatidylcholine in bipolar lipid vesicles.
AuthorsKao YL, Chang EL, Chong PL
JournalBiochem Biophys Res Commun
PubMed ID1445356
The lateral mobility of a pyrene-labeled phosphatidylcholine probe in liposomes containing archaebacterial bipolar lipids has been studied isothermally as a function of pressure. The pressure-dependence of the probe mobility, R, is found to be slightly positive or zero in the temperature range of 17 - 48 degrees C. At temperatures ... More
Influence of the clathrin coat on the membrane lipidic organization of endocytic vesicles: a fluorescence study.
AuthorsLiaubet A, Egret-Charlier M, Kuhry JG
JournalBiochim Biophys Acta
PubMed ID7918559
Endocytic coated vesicles (CV) were purified from bovine brain, and uncoated vesicles (UV) were obtained from the latter by dialysis against 1 M Tris. Membrane dynamics were explored in both vesicle populations using two complementary fluorescence approaches: diphenylhexatriene fluorescence anisotropy to account for rotational lipid movements, and pyrene excimerization with ... More
N-pyrene dodecanoyl sulfatide as membrane probe: a study of glycolipid dynamic behavior in model membranes.
AuthorsViani P, Galimberti C, Marchesini S, Cervato G, Cestaro B
JournalChem Phys Lipids
PubMed ID3342459
An N-linked pyrene-dodecanoyl sulfatide was employed to measure the ratio of excimer fluorescence to monomer fluorescence intensities (E/M). The E/M values provided information about both the dynamic behavior and the structural distribution of the labelled glycolipid in note dispersion of micellar sulfatides and multilamellar vesicles of different phospholipids. Most of ... More
A versatile method for determining the molar ligand-membrane partition coefficient.
AuthorsParry MJ, Jutila A, Kinnunen PK, Alakoskela JM,
JournalJ Fluoresc
PubMed ID17160728
A novel method for the quantitative assessment of the membrane partitioning of a ligand from the aqueous phase is described, demonstrated here with the thoroughly studied antipsychotic chlorpromazine (CPZ). More specifically, collisional quenching of the fluorescence of a pyrene labeled fluorescent lipid analog 1-palmitoyl-2[10-(pyren-1-yl)]decanoyl-sn-glycero-3-phosphocholine (PPDPC) by CPZ was utilized, using ... More
Functional tests for the characterization of surfactant protein B (SP-B) and a fluorescent SP-B analog.
AuthorsDiemel RV, Bader D, Walch M, Hotter B, van Golde LM, Amann A, Haagsman HP, Putz G
JournalArch Biochem Biophys
PubMed ID11368015
Surfactant protein B (SP-B) enhances lipid insertion into the alveolar air/liquid interface upon inhalation. The aim of this study was (i) to apply a palette of tests for a detailed biochemical and biophysical characterization of SP-B and (ii) to use these tests to compare native SP-B with a fluorescent (Bodipy) ... More
Pyrene-labeled lipids as tools in membrane biophysics and cell biology.
AuthorsSomerharju P
JournalChem Phys Lipids
PubMed ID12093535
Pyrene is one of the most frequently used lipid-linked fluorophores. Its most characteristic features are a long excited state lifetime and (local) concentration-dependent formation of excimers. Pyrene is also hydrophobic and thus does not significantly distort the conformation of the labeled lipid molecule. These characteristics make pyrene lipids well-suited for ... More
Membrane binding mechanism of an RNA virus-capping enzyme.
AuthorsLampio A, Kilpeläinen I, Pesonen S, Karhi K, Auvinen P, Somerharju P, Kääriäinen L
JournalJ Biol Chem
PubMed ID10984480
The RNA replication complex of Semliki Forest virus is bound to cytoplasmic membranes via the mRNA-capping enzyme Nsp1. Here we have studied the structure and liposome interactions of a synthetic peptide (245)GSTLYTESRKLLRSWHLPSV(264) corresponding to the membrane binding domain of Nsp1. The peptide interacted with liposomes only if negatively charged lipids ... More
Free volume model for lipid lateral diffusion coefficients. Assessment of the temperature dependence in phosphatidylcholine and phosphatidylethanolamine bilayers.
AuthorsKing MD, Marsh D
JournalBiochim Biophys Acta
PubMed ID3768368
The lipid lateral diffusion coefficients, DT, in fluid-phase phosphatidylcholine and phosphatidylethanolamine bilayers have been analysed in terms of the free-volume diffusion model by fitting the expression: DT = AT exp[- B/(T - T0)] to the observed temperature dependence, where A, B and T0 are the parameters to be optimized. Application ... More
Enzyme II of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system: protein-protein and protein-phospholipid interactions.
AuthorsRobillard GT, Blaauw M
JournalBiochemistry
PubMed ID3314996
The mannitol-specific enzyme II (EII), purified free of phospholipid, exhibits a concentration dependence in its specific activity with P-HPr and mannitol as the donor and acceptor substrates, respectively. This concentration dependence, previously observed only in the case of mannitol----mannitol phosphate exchange reaction, indicates that an oligomeric form of the enzyme ... More
Functional characteristics of a phospholipase A(2) inhibitor from Notechis ater serum.
AuthorsHains PG, Sung KL, Tseng A, Broady KW
JournalJ Biol Chem
PubMed ID10625636
A phospholipase A(2) inhibitor has been purified p6om the serum of Notechis ater using DEAE-Sephacel chromatography. The inhibitor was found to be composed of two protein subunits (alpha and beta) that form the intact complex of approximately 110 kDa. The alpha-chain is a 30-kDa glycoprotein and the beta-chain a nonglycosylated, ... More
Association of lysozyme to phospholipid surfaces and vesicle fusion.
AuthorsArnold K, Hoekstra D, Ohki S
JournalBiochim Biophys Acta
PubMed ID1543731
Lysozyme-induced fusion of phosphatidylserine (PS) vesicles was studied as a function of pH. Fusion, monitored by lipid-mixing, was measured by following the dilution of pyrene-labelled phosphatidylcholine, incorporated in PS vesicles, into unlabelled bilayers. It is demonstrated that lysozyme-induced fusion is pH-dependent and significant fusion is triggered at pH 5 or ... More
A sensitive and continuous fluorometric assay for phospholipase A2 using pyrene-labeled phospholipids in the presence of serum albumin.
AuthorsRadvanyi F, Jordan L, Russo-Marie F, Bon C
JournalAnal Biochem
PubMed ID2742139
Phospholipase A2 activity can be determined fluorometrically in the presence of serum albumin using phospholipids labeled at the sn-2-acyl position with 10-pyrenyldecanoic acid. In the water reaction medium 10-pyrene phospholipids form vesicles and the monomer fluorescence of the pyrene is negligible due to pyrene-pyrene interaction. Upon phospholipid hydrolysis 10-pyrenyldecanoic acids ... More
Role of cholesterol in the modulation of interdigitation in phosphatidylethanols.
AuthorsBondar OP, Rowe ES
JournalBiochim Biophys Acta
PubMed ID9545567
Phosphatidylethanol (Peth) is formed in biological membranes when ethanol replaces water in the transphosphatidylation reaction catalyzed by phospholipase D. This charged lipid accumulates in the presence of ethanol, and it has unusual properties that can influence membrane structure and function. We have previously shown that dimyristoylphosphatidylethanol (DMPeth) and dipalmitoylphosphatidylethanol (DPPeth) ... More
Dimeric N-terminal segment of human surfactant protein B (dSP-B(1-25)) has enhanced surface properties compared to monomeric SP-B(1-25).
AuthorsVeldhuizen EJ, Waring AJ, Walther FJ, Batenburg JJ, van Golde LM, Haagsman HP
JournalBiophys J
PubMed ID10866963
Surfactant protein B (SP-B) is a 17-kDa dimeric protein produced by alveolar type II cells. Its main function is to lower the surface tension by inserting lipids into the air/liquid interface of the lung. SP-B's function can be mimicked by a 25-amino acid peptide, SP-B(1-25), which is based on the ... More
Novel lipid transfer property of two mitochondrial proteins that bridge the inner and outer membranes.
AuthorsEpand RF, Schlattner U, Wallimann T, Lacombe ML, Epand RM
JournalBiophys J
PubMed ID17028143
This study provides evidence of a novel function for mitochondrial creatine kinase (MtCK) and nucleoside diphosphate kinase (NDPK-D). Both are basic peripheral membrane proteins with symmetrical homo-oligomeric structure, which in the case of MtCK was already shown to allow crossbridging of lipid bilayers. Here, different lipid dilution assays clearly demonstrate ... More
Oxygen quenching of pyrene-lipid fluorescence in phosphatidylcholine vesicles. A probe for membrane organization.
AuthorsChong PL, Thompson TE
JournalBiophys J
PubMed ID4016182
Oxygen quenching has been used as an alternative method to study the temperature dependence of the apparent excimer formation constant, kdm, of N-(10-[1-pyrene]-decanoyl)-sphingomyelin (Pyr-SPM) in 1-palmitoyl-2-oleoyl-L-alpha-phosphatidylcholine (POPC) multilamellar vesicles. In conjunction with the lifetime of Pyr-SPM monomer in the absence of excimer and oxygen, kdm can be determined from the ... More
Fluorescence studies of lipid regular distribution in membranes.
AuthorsChong PL, Sugár IP
JournalChem Phys Lipids
PubMed ID12093540
This article reviews the use of fluorescent lipids and free probes in the studies of lipid regular distribution in model membranes. The first part of this article summarizes the evidence and physical properties for lipid regular distribution in pyrene-labeled phosphatidylcholine (PC)/unlabeled PC binary mixtures as revealed by the fluorescence of ... More
The molecular basis of phosphatidylcholine preference of human group-V phospholipase A2.
AuthorsKim KP, Han SK, Hong M, Cho W
JournalBiochem J
PubMed ID10839997
Human group-V phospholipase A(2) (hVPLA(2)) is a secretory phospholipase A(2) (PLA(2)) that is involved in eicosanoid formation in such inflammatory cells as macrophages and mast cells. We showed that hVPLA(2) can bind phosphatidylcholine membranes and hydrolyse phosphatidylcholine molecules much more efficiently than human group-IIa PLA(2), which accounts for its high ... More
Active serine involved in the stabilization of the active site loop in the Humicola lanuginosa lipase.
AuthorsPeters GH, Svendsen A, Langberg H, Vind J, Patkar SA, Toxvaerd S, Kinnunen PK
JournalBiochemistry
PubMed ID9730809
We have investigated the binding properties of and dynamics in Humicola lanuginosa lipase (Hll) and the inactive mutant S146A (active Ser146 substituted with Ala) using fluorescence spectroscopy and molecular dynamics simulations, respectively. Hll and S146A show significantly different binding behavior for phosphatidylcholine (PC) and phosphatidylglycerol (PG) liposomes. Generally, higher binding ... More
A continuous fluorometric assay for phospholipase A(2) activity in brain cytosol.
AuthorsYarger DE, Patrick CB, Rapoport SI, Murphy EJ
JournalJ Neurosci Methods
PubMed ID11040375
Alterations in phospholipase A(2) (PLA(2)) activity have been implicated in Alzheimer disease and other neurological disorders, although brain PLA(2) activity is currently measured using lengthy, non-continuous assays. We describe herein a rapid, continuous assay in which we measured PLA(2) activity in mouse brain cytosol (CB-57). Brains were homogenized in HEPES ... More
Neutralization of the positive charges of surfactant protein C. Effects on structure and function.
AuthorsCreuwels LA, Boer EH, Demel RA, van Golde LM, Haagsman HP
JournalJ Biol Chem
PubMed ID7608188
Pulmonary surfactant protein C (SP-C) is a small, extremely hydrophobic peptide with a highly conservative primary structure. The protein is characterized by two adjacent palmitoylated cysteine residues, two positively charged residues (one arginine residue and one lysine residue) in the N-terminal region, and a long hydrophobic stretch. SP-C enhances the ... More
Interactions of annexins with membrane phospholipids.
AuthorsMeers P, Daleke D, Hong K, Papahadjopoulos D
JournalBiochemistry
PubMed ID1826088
The annexins are proteins that bind to membranes and can aggregate vesicles and modulate fusion rates in a Ca2(+)-dependent manner. In this study, experiments are presented that utilize a pyrene derivative of phosphatidylcholine to examine the Ca2(+)-dependent membrane binding of soluble human annexin V and other annexins. When annexin V ... More
Fluorescence imaging of pyrene-labeled lipids in living cells.
AuthorsTanhuanpää K, Virtanen J, Somerharju P
JournalBiochim Biophys Acta
PubMed ID10996655
Microscopic imaging of fluorescent lipid derivatives is a powerful tool to study membrane organization and lipid trafficking but it is complicated by cellular autofluorescence background and photobleaching of the fluorophore as well as by the difficulty to selectively image membranes stacked on top of each other. Here we describe protocols ... More
An animal virus-derived peptide switches membrane morphology: possible relevance to nodaviral transfection processes.
AuthorsJanshoff A, Bong DT, Steinem C, Johnson JE, Ghadiri MR
JournalBiochemistry
PubMed ID10220319
The N-terminal domain of the capsid protein cleavage product of the flock house virus (FHV) consists of 21 residues and forms an amphipathic alpha-helix, which is thought to play a crucial role in permeabilizing biological membranes for RNA translocation in the host cell. We have found that the Met --> ... More
A hydrophobic cluster at the surface of the human plasma phospholipid transfer protein is critical for activity on high density lipoproteins.
AuthorsDesrumaux C, Labeur C, Verhee A, Tavernier J, Vandekerckhove J, Rosseneu M, Peelman F
JournalJ Biol Chem
PubMed ID11083872
The plasma phospholipid transfer protein (PLTP) belongs to the lipid transfer/lipopolysaccharide binding protein (LT/LBP) family, together with the cholesteryl ester transfer protein, the lipopolysaccharide binding protein (LBP) and the bactericidal permeability increasing protein (BPI). In the present study, we used the crystallographic data available for BPI to build a three-dimensional ... More
Binding of crotoxin, a presynaptic phospholipase A2 neurotoxin, to negatively charged phospholipid vesicles.
AuthorsRadvanyi F, Saliou B, Lembezat MP, Bon C
JournalJ Neurochem
PubMed ID2769265
Crotoxin, isolated from the venom of Crotalus durissus terrificus, is a potent neurotoxin consisting of a basic and weakly toxic phospholipase A2 subunit (component B) and an acidic nonenzymatic subunit (component A). The nontoxic component A enhances the toxicity of the phospholipase subunit by preventing its nonspecific adsorption. The binding ... More
On the role of ligand in retinoid signaling: positive cooperativity in the interactions of 9-cis retinoic acid with tetramers of the retinoid X receptor.
AuthorsKersten S, Pan L, Noy N
JournalBiochemistry
PubMed ID7578026
Previously, we have shown that the retinoid X receptor (RXR) forms tetramers with a high affinity and that interactions of the receptor with its ligand, 9-cis retinoic acid (9cRA), result in dissociation of protein tetramers. Here it is shown by fluorescence anisotropy studies that ligand-induced tetramer dissociation displays a pronounced ... More
Lateral distribution of a pyrene-labeled phosphatidylcholine in phosphatidylcholine bilayers: fluorescence phase and modulation study.
AuthorsHresko RC, Sugár IP, Barenholz Y, Thompson TE
JournalBiochemistry
PubMed ID3741837
The lateral distribution of 1-palmitoyl-2-[10-(1-pyrenyl)decanoyl]phosphatidylcholine (PyrPC) was studied in small unilamellar vesicles of 1,2-dipalmitoyl-, 1,2-dimyristoyl-, and 1-palmitoyl-2-oleoyl-phosphatidylcholine (DPPC, DMPC, and POPC, respectively) under anaerobic conditions. The DPPC and DMPC experiments were carried out over temperature ranges above and below the matrix phospholipid phase transition temperature (Tm). The excimer to monomer ... More
Pyrene phospholipid as a biological fluorescent probe for studying fusion of virus membrane with liposomes.
AuthorsPal R, Barenholz Y, Wagner RR
JournalBiochemistry
PubMed ID2831956
We are using fluorescent endogenous phospholipids in virus membranes to study the factors that promote fusion on interaction with receptor membranes. To this end, vesicular stomatitis virus (VSV) grown in baby hamster kidney (BHK-21) cells was biologically labeled with fluorescent lipids, primarily phosphatidylcholine and phosphatidylethanolamine, derived from pyrene fatty acids. ... More
Stimulation of cellular sphingomyelin import by the chemokine connective tissue-activating peptide III.
AuthorsStoeckelhuber M, Dobner P, Baumgartner P, Ehlert J, Brandt E, Mentele R, Adam D, Engelmann B
JournalJ Biol Chem
PubMed ID10956644
The selective import of phospholipids into cells could be mediated by proteins secreted from the cells into the extracellular compartment. We observed that the supernatants obtained from suspensions of thrombin-activated platelets stimulated the exchange of pyrene (py)-labeled sphingomyelin between lipid vesicles in vitro. The proteins with sphingomyelin transfer activity were ... More
Phosphoinositide-protein interactions of the plasma-membrane Ca2(+)-transport ATPase as revealed by fluorescence energy transfer.
Fluorescence energy transfer has been used to study the interaction of various phospholipids with the erythrocyte (Ca2+ + Mg2+)-ATPase. The fluorescence energy transfer between tryptophan residues of the (Ca2+ + Mg2+)-ATPase purified from erythrocytes and pyrene-labelled analogues of phosphatidylcholine (Pyr-PC), phosphatidylinositol (Pyr-PI), phosphatidylinositol 4-phosphate (Pyr-PIP), phosphatidylinositol 4,5-bisphosphate (Pyr-PIP2), phosphatidylglycerol (Pyr-PG) ... More
Importance of phosphatidylethanolamine for the interaction of apocytochrome c with model membranes containing phosphatidylserine.
AuthorsAhn T, Oh DB, Lee BC, Yun CH
JournalBiochemistry
PubMed ID10956003
The effect of phosphatidylethanolamine (PE) on the binding of apocytochrome c to model membranes was examined. When 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) of the standard vesicles composed of 80% of this lipid and 20% of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine (POPS) was gradually replaced with upward of 50% of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), the binding increased appreciably. Ca(2+), causing ... More
Location of tryptophans in membrane-bound annexins.
AuthorsMeers P
JournalBiochemistry
PubMed ID2139793
The annexins are a novel class of calcium-dependent membrane binding proteins with highly homologous sequences and similar binding characteristics. In order to better define structural parameters of the membrane-bound form, the localization of tryptophan residues in several of these proteins was studied by use of quenchers of their intrinsic fluorescence. ... More
Relationship between annexin V tryptophan exposure, calcium, and phospholipid binding.
AuthorsMeers P, Mealy T
JournalBiochemistry
PubMed ID8499444
Annexin V is a Ca(2+)-dependent phospholipid-binding protein that may have one or more membrane-related functions, including inhibition of blood coagulation. The fluorescence of the single tryptophan of annexin V was used to monitor Ca2+ and/or phospholipid binding in terms of emission wavelength, emission intensity, and susceptibility to acrylamide quenching. In ... More
Quantitation of lateral stress in lipid layer containing nonbilayer phase preferring lipids by frequency-domain fluorescence spectroscopy.
AuthorsChen SY, Cheng KH, Van der Meer BW
JournalBiochemistry
PubMed ID1567830
Frequency-resolved fluorescence measurements have been performed to quantitate the lateral stress of the lipid layer containing nonbilayer phase preferring dioleoylphosphatidylethanolamine (DOPE). On the basis of a new rotational diffusion model, the wobbling diffusion constant (Dw), the curvature-related hopping diffusion constant (DH), and the two local orientational order parameters ([P2] and ... More
Lipid microdomains in dimyristoylphosphatidylcholine-ceramide liposomes.
AuthorsHolopainen JM, Lehtonen JY, Kinnunen PK
JournalChem Phys Lipids
PubMed ID9297850
Binary membranes composed of dimyristoylphosphatidylcholine (DMPC) and natural ceramide (up to a mole fraction Xcer = 0.25) were investigated by measuring the excimer:monomer fluorescence emission intensity ratio IE:IM for the pyrene labeled phospholipid probe 1-palmitoyl-2[(pyren-1-yl)]decanoyl-sn-glycero-3-phosphocholine (PPDPC), by monitoring fluorescence polarization of diphenylhexatriene (DPH), as well as using differential scanning calorimetry ... More
Pyrene lipids as markers of peroxidative processes in different regions of low and high density lipoproteins.
AuthorsViani P, Cazzola R, Cervato G, Gatti P, Cestaro B
JournalBiochim Biophys Acta
PubMed ID8608174
Three different pyrene derivatives, pyrene decanoyl phosphatidylcholine (P10PC), pyrene dodecanoyl sulfatide (P12CS) and cholesteryl pyrenyl hexanoate (P6Chol), were used to follow lipid peroxidation in low and high density lipoproteins. Probe-labelled lipoproteins were subjected to Cu2+ catalyzed peroxidation. In all cases the fluorescence of the probes progressively decreased due to the ... More
Evaluation of viral membrane fusion assays. Comparison of the octadecylrhodamine dequenching assay with the pyrene excimer assay.
AuthorsStegmann T, Schoen P, Bron R, Wey J, Bartoldus I, Ortiz A, Nieva JL, Wilschut J
JournalBiochemistry
PubMed ID8218197
Membrane fusion, in particular the fusion of enveloped viruses, is often measured with an assay based on octadecylrhodamine (R18) fluorescence dequenching. We have studied the association of R18 with membranes and used the R18 assay to measure virus fusion in model systems and in cultured cells. The results were compared ... More
Effect of cholesterol on the ethanol-induced interdigitated gel phase in phosphatidylcholine: use of fluorophore pyrene-labeled phosphatidylcholine.
AuthorsKomatsu H, Rowe ES
JournalBiochemistry
PubMed ID2001373
It is now recognized that many amphiphilic molecules such as ethanol can induce the formation of the fully interdigitated gel phase (L beta I) in phosphatidylcholines (PC's). In the present study, we have developed a simple detection method for the L beta I phase using pyrene-labeled PC (PyrPC), which is ... More
Properties of the binding sites for the sn-1 and sn-2 acyl chains on the phosphatidylinositol transfer protein from bovine brain.
We have studied the properties of the fatty acyl binding sites of the phosphatidylinositol transfer protein (PI-TP) from bovine brain, by measuring the binding and transfer of pyrenylacyl-containing phosphatidylinositol (PyrPI) species and pyrenylacyl-containing phosphatidylcholine (PyrPC) species as a function of the acyl chain length. The PyrPI species carried a pyrene-labeled ... More