Endotoxin induces expression of type II phospholipase A2 in macrophages during acute lung injury in guinea pigs: involvement of TNF-alpha in lipopolysaccharide-induced type II phospholipase A2 synthesis.
AuthorsArbibe L, Vial D, Rosinski-Chupin I, Havet N, Huerre M, Vargaftig BB, Touqui L
JournalJ Immunol
PubMed ID9200478
Elevated levels of secretory type II phospholipase A2 (sPLA2-II) have been associated with a poor clinical outcome in the acute respiratory distress syndrome. This study identifies the cell source(s) and the mechanisms of sPLA2-II synthesis in the guinea pig model of acute respiratory distress syndrome induced by intratracheal injection of ... More
Mapping the interfacial binding surface of human secretory group IIa phospholipase A2.
AuthorsSnitko Y, Koduri RS, Han SK, Othman R, Baker SF, Molini BJ, Wilton DC, Gelb MH, Cho W
JournalBiochemistry
PubMed ID9398150
Human secretory group IIa phospholipase A2 (hIIa-PLA2) contains a large number of prominent cationic patches on its molecular surface and has exceptionally high affinity for anionic surfaces, including anionic membranes. To identify the cationic amino acid residues that support binding of hIIa-PLA2 to anionic membranes, we have performed extensive site-directed ... More
Molecular and macromolecular specificity of human plasma phospholipid transfer protein.
AuthorsRao R, Albers JJ, Wolfbauer G, Pownall HJ
JournalBiochemistry
PubMed ID9132017
'Phospholipid transfer protein (PLTP), also known as lipid transfer protein 2 (LTP-2), mediates a transfer of phospholipids between high-density lipoproteins (HDL). The molecular and macromolecular specificities of recombinant human PLTP were studied using a fluorometric assay based on the excimer fluorescence of pyrenyl lipids. To determine lipoprotein specificity of PLTP, ... More
Differential interfacial and substrate binding modes of mammalian pancreatic phospholipases A2: a comparison among human, bovine, and porcine enzymes.
AuthorsSnitko Y, Han SK, Lee BI, Cho W
JournalBiochemistry
PubMed ID10387020
'To identify the residues essential for interfacial binding and substrate binding of human pancreatic phospholipase A2 (hpPLA2), several ionic residues in the putative interfacial binding surface (R6E, K7E, K10E, and K116E) and substrate binding site (D53K and K56E) were mutated. Interfacial affinity of these mutants was measured using anionic polymerized ... More
Use of polymerized mixed liposomes to study interactions of phospholipase A2 with membranes.
AuthorsWu SK, Cho W
JournalBiochemistry
PubMed ID8268165
'Polymerized liposomes of thiol-based phospholipids, 1,2-bis[12-(lipoyloxy)dodecanoyl]-sn-glycero-3-phosphocholine (BLPC) and -phosphoglycerol (BLPG) were used to study interactions of several phospholipases A2 (PLA2) with membranes. Large liposomes (an average diameter of 100 +/- 10 nm) prepared from BLPC or BLPG were readily hydrolyzed by PLA2. Once polymerized, however, these liposomes were resistant to ... More
Binding and dissociation of cytochrome c to and from membranes containing acidic phospholipids.
AuthorsSubramanian M, Jutila A, Kinnunen PK
JournalBiochemistry
PubMed ID9477968
'Membrane association and detachment of cytochrome c (cyt c) in millisecond to second time domain were investigated by stopped-flow fluorescence spectroscopy monitoring the efficiency of energy transfer from a pyrene-fatty acid containing phospholipid derivative, 1-palmitoyl-2-[10-(pyren-1-yl)-decanoyl]-sn-glycero-3-phosphoglyce rol (PPDPG, mole fraction X = 0.01) to the heme of the cyt c. Large ... More
The differential regulation of group II(A) and group V low molecular weight phospholipases A(2) in cultured rat astrocytes.
AuthorsThomas G, Bertrand F, Saunier B
JournalJ Biol Chem
PubMed ID10753884
'In astrocytes, cytokines stimulate the release of secretory phospholipase A(2) (sPLA(2)) activity and group II(A) sPLA(2) expression. This paper reports that two sPLA(2) isoforms, group II(A) and group V, are in fact expressed by astrocytes. Our studies showed that tumor necrosis factor alpha (TNFalpha) enhanced the mRNA of both isoforms, ... More
Characteristics of the binding of tacrine to acidic phospholipids.
AuthorsLehtonen JY, Rytömaa M, Kinnunen PK
JournalBiophys J
PubMed ID9172742
'Tacrine (1,2,3,4-tetrahydro-9-acridinamine monohydrate) is an inhibitor of acetylcholinesterase currently used in the treatment of the symptoms of Alzheimer's disease. The present study demonstrates preferential binding of this drug to acidic phospholipids, as revealed by fluorescence polarization, penetration into lipid monolayers, and effects on the thermal phase behavior of dimyristoyl phosphatidic ... More
Solution structure of Ace-AMP1, a potent antimicrobial protein extracted from onion seeds. Structural analogies with plant nonspecific lipid transfer proteins.
AuthorsTassin S, Broekaert WF, Marion D, Acland DP, Ptak M, Vovelle F, Sodano P
JournalBiochemistry
PubMed ID9521681
'The three-dimensional solution structure of Ace-AMP1, an antifungal protein extracted from onion seeds, was determined using 1H NMR spectroscopy and molecular modeling. This cationic protein contains 93 amino acid residues and four disulfide bridges. Its structure was determined from 1260 NOE-derived distance restraints and 173 dihedral restraints derived from NOEs ... More
Binding of endostatin to phosphatidylserine-containing membranes and formation of amyloid-like fibers.
AuthorsZhao H, Jutila A, Nurminen T, Wickström SA, Keski-Oja J, Kinnunen PK
JournalBiochemistry
PubMed ID15723529
'Endostatin, the 20-kDa C-terminal NC1 domain of collagen XVIII, is an endogenous inhibitor of tumor angiogenesis and tumor growth. A major problem in reconciling the many reported in vitro effects of endostatin is the lack of a high-affinity receptor, and a search for the latter continues. In accordance with the ... More
Phospholipase A2 inhibition at different substrate concentrations.
AuthorsBöhl M, Ketscher L, Tietböhl C, Gutzeit HO
JournalAnal Biochem
PubMed ID17045950
Pyrene-labeled lipids as tools in membrane biophysics and cell biology.
AuthorsSomerharju P
JournalChem Phys Lipids
PubMed ID12093535
Pyrene is one of the most frequently used lipid-linked fluorophores. Its most characteristic features are a long excited state lifetime and (local) concentration-dependent formation of excimers. Pyrene is also hydrophobic and thus does not significantly distort the conformation of the labeled lipid molecule. These characteristics make pyrene lipids well-suited for ... More
Functional characteristics of a phospholipase A(2) inhibitor from Notechis ater serum.
AuthorsHains PG, Sung KL, Tseng A, Broady KW
JournalJ Biol Chem
PubMed ID10625636
A phospholipase A(2) inhibitor has been purified p6om the serum of Notechis ater using DEAE-Sephacel chromatography. The inhibitor was found to be composed of two protein subunits (alpha and beta) that form the intact complex of approximately 110 kDa. The alpha-chain is a 30-kDa glycoprotein and the beta-chain a nonglycosylated, ... More
Inhibition of secreted phospholipase A2. 4-glycerol derivatives of 4,5-dihydro-3-(4-tetradecyloxybenzyl)-1,2,4-4H-oxadiazol-5-one with broad activities.
AuthorsTouaibia M, Djimdé A, Cao F, Boilard E, Bezzine S, Lambeau G, Redeuilh C, Lamouri A, Massicot F, Chau F, Dong CZ, Heymans F
JournalJ Med Chem
PubMed ID17335183
Secreted phospholipases A2 (sPLA2s) have been reported to play an important role in various inflammatory conditions and thus represent an attractive therapeutic target. Previous SAR studies from our laboratory have revealed certain important features of our recently discovered specific hGIIA sPLA2 inhibitors, and we report here the synthesis and biological ... More
Novel lipid transfer property of two mitochondrial proteins that bridge the inner and outer membranes.
AuthorsEpand RF, Schlattner U, Wallimann T, Lacombe ML, Epand RM
JournalBiophys J
PubMed ID17028143
This study provides evidence of a novel function for mitochondrial creatine kinase (MtCK) and nucleoside diphosphate kinase (NDPK-D). Both are basic peripheral membrane proteins with symmetrical homo-oligomeric structure, which in the case of MtCK was already shown to allow crossbridging of lipid bilayers. Here, different lipid dilution assays clearly demonstrate ... More
The molecular basis of phosphatidylcholine preference of human group-V phospholipase A2.
AuthorsKim KP, Han SK, Hong M, Cho W
JournalBiochem J
PubMed ID10839997
Human group-V phospholipase A(2) (hVPLA(2)) is a secretory phospholipase A(2) (PLA(2)) that is involved in eicosanoid formation in such inflammatory cells as macrophages and mast cells. We showed that hVPLA(2) can bind phosphatidylcholine membranes and hydrolyse phosphatidylcholine molecules much more efficiently than human group-IIa PLA(2), which accounts for its high ... More
Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2.
AuthorsSinger AG, Ghomashchi F, Le Calvez C, Bollinger J, Bezzine S, Rouault M, Sadilek M, Nguyen E, Lazdunski M, Lambeau G, Gelb MH
JournalJ Biol Chem
PubMed ID12359733
Expression of the full set of human and mouse groups I, II, V, X, and XII secreted phospholipases A(2) (sPLA(2)s) in Escherichia coli and insect cells has provided pure recombinant enzymes for detailed comparative interfacial kinetic and binding studies. The set of mammalian sPLA(2)s display dramatically different sensitivity to dithiothreitol. ... More
High-performance liquid chromatography determination of bis(monoacylglycerol) phosphate and other lysophospholipids.
AuthorsLuquain C, Laugier C, Lagarde M, Pageaux JF
JournalAnal Biochem
PubMed ID11520030
Bis(monoacylglycerol) phosphate (BMP) is a very minor component of the phospholipid (PL) fraction in rat uterine stromal cell cultures (U(III) cells). Under several culture conditions, including the addition of (n-3) or (n-6) polyunsaturated fatty acids, BMP selectively accumulates docosahexaenoic acid (DHA). We have recently described the structure of this PL, ... More
The annexin A3-membrane interaction is modulated by an N-terminal tryptophan.
AuthorsHofmann A, Raguénès-Nicol C, Favier-Perron B, Mesonero J, Huber R, Russo-Marie F, Lewit-Bentley A
JournalBiochemistry
PubMed ID10869176
The crystal structure of annexin A3 (human annexin III) solved recently revealed a well-ordered folding of its N-terminus with the side chain of tryptophan 5 interacting with residues at the extremity of the central pore. Since the pore of annexins has been suggested as the ion pathway involved in membrane ... More
Roles of aromatic residues in high interfacial activity of Naja naja atra phospholipase A2.
AuthorsSumandea M, Das S, Sumandea C, Cho W
JournalBiochemistry
PubMed ID10587453
Acidic phospholipase A2 (PLA2) from the venom of Chinese cobra (Naja naja atra) has high activity on zwitterionic membranes and contains six aromatic residues, including Tyr-3, Trp-18, Trp-19, Trp-61, Phe-64, and Tyr-110, on its putative interfacial binding surface. To assess the roles of these aromatic residues in the interfacial catalysis ... More
A structural determinant of the unique interfacial binding mode of bovine pancreatic phospholipase A2.
AuthorsLee BI, Dua R, Cho W
JournalBiochemistry
PubMed ID10387021
The catalytic steps of the phospholipase A2 (PLA2)-catalyzed hydrolysis of phospholipids are preceded by interfacial binding. Among various pancreatic PLA2s, bovine pancreatic PLA2 (bpPLA2) has a unique interfacial binding mode in which Lys-56 plays an important role in its binding to anionic lipid surfaces. To identify the structural determinant of ... More
A fluorescence spectroscopy study on the interactions of the TAT-PTD peptide with model lipid membranes.
AuthorsTiriveedhi V, Butko P
JournalBiochemistry
PubMed ID17338552
Protein-transduction domains (PTDs) have been shown to translocate into and through the living cells in a rapid manner by an as yet unknown mechanism. Regardless of the mechanism of translocation, the first necessary step must be binding of the PTD peptide to the surface of the lipid membrane. We used ... More
Interaction of alpha-and beta-oligoarginine-acids and amides with anionic lipid vesicles: a mechanistic and thermodynamic study.
The interaction of alpha- and beta-oligoarginine amides and acids and of alpha-polyarginine with anionic lipid vesicles was studied. The beta-oligoarginines used were beta3-homologues of the alpha-oligoarginines. Lipid bilayers were composed of POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) and POPG (1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)]) containing 5 mol % pyrene-PG (1-hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-[phospho-rac-1-glycerol]). Kinetic analysis of the binding process onto large ... More
Structural aspects of interfacial adsorption. A crystallographic and site-directed mutagenesis study of the phospholipase A2 from the venom of Agkistrodon piscivorus piscivorus.
AuthorsHan SK, Yoon ET, Scott DL, Sigler PB, Cho W
JournalJ Biol Chem
PubMed ID9013608
Recent genetic and structural studies have shed considerable light on the mechanism by which secretory phospholipases A2 interact with substrate aggregates. Electrostatic forces play an essential role in optimizing interfacial catalysis. Efficient and productive adsorption of the Class I bovine pancreatic phospholipase A2 to anionic interfaces is dependent upon the ... More
Domain formation in a fluid mixed lipid bilayer modulated through binding of the C2 protein motif.
AuthorsHinderliter A, Almeida PF, Creutz CE, Biltonen RL
JournalBiochemistry
PubMed ID11300799
The role and mechanism of formation of lipid domains in a functional membrane have generally received limited attention. Our approach, based on the hypothesis that thermodynamic coupling between lipid-lipid and protein-lipid interactions can lead to domain formation, uses a combination of an experimental lipid bilayer model system and Monte Carlo ... More
Detachment of cytochrome c by cationic drugs from membranes containing acidic phospholipids: comparison of lidocaine, propranolol, and gentamycin.
AuthorsJutila A, Rytömaa M, Kinnunen PK
JournalMol Pharmacol
PubMed ID9765516
A large number of pharmaceutically active compounds have a high affinity to acidic phospholipids; good examples are the cationic compounds lidocaine, propranolol, and gentamycin. These drugs influenced the lipid dynamics of liposomes composed of phosphatidylcholine and the acidic phosphatidylglycerol, as judged by the excimer/monomer emission intensity ratio for a pyrene-labeled ... More
Interleukin 1beta induces type II-secreted phospholipase A(2) gene in vascular smooth muscle cells by a nuclear factor kappaB and peroxisome proliferator-activated receptor-mediated process.
AuthorsCouturier C, Brouillet A, Couriaud C, Koumanov K, Béréziat G, Andréani M
JournalJ Biol Chem
PubMed ID10438477
Type II-secreted phospholipase A(2) (type II-sPLA(2)) is expressed in smooth muscle cells during atherosclerosis or in response to interleukin-1beta. The present study shows that the induction of type II-sPLA(2) gene by interleukin-1beta requires activation of the NFkappaB pathway and cytosolic PLA(2)/PPARgamma pathway, which are both necessary to achieve the transcriptional ... More
A structure-function study of bovine pancreatic phospholipase A2 using polymerized mixed liposomes.
AuthorsDua R, Wu SK, Cho W
JournalJ Biol Chem
PubMed ID7814384
A new combinatorial approach that includes the genetic variation of protein structure and the chemical modification of phospholipid structure in polymerized mixed liposomes was used to delineate the structure-function relationships in the interfacial catalysis of bovine pancreatic phospholipase A2 (PLA2). Based on previous structural and mutational studies, several bovine PLA2 ... More
Comparison of the membrane association of two antimicrobial peptides, magainin 2 and indolicidin.
Interactions of two antimicrobial peptides, magainin 2 and indolicidin, with three different model biomembranes, namely, monolayers, large unilamellar vesicles (LUVs), and giant liposomes, were studied. Insertion of both peptides into lipid monolayers was progressively enhanced when the content of an acidic phospholipid, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG) in a film of 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine (SOPC) ... More