Poly(beta-amino ester)s promote cellular uptake of heparin and cancer cell death.
AuthorsBerry D, Lynn DM, Sasisekharan R, Langer R
JournalChem Biol
PubMed ID15123243
'Heparin/heparan sulfate-like glycosaminoglycans (HSGAGs) are involved in diverse cellular processes in the extracellular matrix (ECM). The biological effect of HSGAGs depends on disaccharide content and physiological location within the ECM. HSGAGs are also brought into cells during membrane transcytosis and growth factor signaling while protein bound. We sought to probe ... More
Solid-phase synthesis of fluorescent heparin.
AuthorsRadoff S, Danishefsky I
JournalAnal Biochem
PubMed ID7091663
Heparin binding is necessary, but not sufficient, for fibronectin aggregation. A fluorescence polarization study.
AuthorsBentley KL, Klebe RJ, Hurst RE, Horowitz PM
JournalJ Biol Chem
PubMed ID3997865
'Analysis of parameters governing heparin binding to fibronectin indicates that heparin binding is a necessary, but insufficient, condition for fibronectin cryoprecipitation. Heparin binding to fibronectin is a rapid, readily reversible event which can occur under several conditions which prohibit fibronectin cryoprecipitation. While cryoprecipitation of fibronectin is abolished at temperatures in ... More
Binding and internalization of heparin by vascular smooth muscle cells.
AuthorsCastellot JJ, Wong K, Herman B, Hoover RL, Albertini DF, Wright TC, Caleb BL, Karnovsky MJ
JournalJ Cell Physiol
PubMed ID3930515
'Previous work from our laboratory has demonstrated that heparin specifically inhibits the proliferation of vascular smooth muscle cells in vivo and in vitro. In this paper, we examine the binding and mode of internalization of heparin by smooth muscle cells. For these studies, radiolabeled and fluoresceinated (FITC) heparin probes were ... More
Heparin binding by fibronectin module III-13 involves six discontinuous basic residues brought together to form a cationic cradle.
AuthorsBusby TF, Argraves WS, Brew SA, Pechik I, Gilliland GL, Ingham KC
JournalJ Biol Chem
PubMed ID7629186
'The thirteenth type III domain of fibronectin binds heparin almost as well as fibronectin itself and contains a so-called heparin-binding consensus sequence, Arg6-Arg7-Ala8-Arg9 (residues 1697-1700 in plasma fibronectin). Barkalow and Schwarzbauer (Barkalow, F.J., and Schwarzbauer, J.E. (1991) J. Biol. Chem. 266, 7812-7818) showed that mutation of Arg6-Arg7 in domain III-13 ... More
Local intracoronary heparin delivery with a microporous balloon catheter.
AuthorsThomas CN, Robinson KA, Cipolla GD, King SB, Scott NA
JournalAm Heart J
PubMed ID8892769
'Arterial thrombosis plays a major role in the pathogenesis of acute coronary syndromes such as unstable angina and acute myocardial infarction. Heparin is efficacious in treating both disorders; however, systemically administered heparin is associated with bleeding complications. Local intracoronary delivery of heparin may be a safer, more effective method of ... More
Multiple complexes of thrombin and heparin.
AuthorsEvington JR, Feldman PA, Luscombe M, Holbrook JJ
JournalBiochim Biophys Acta
PubMed ID3697371
'Fluorescence polarization has been used to study the interaction of thrombin and heparin, and the catalysis by heparin of the combination of thrombin and antithrombin. At low ionic strength (20 mM Tris, pH 7.4), the addition of heparins of known molecular weights to thrombin led to the formation of large ... More
Localized intramural drug delivery during balloon angioplasty using hydrogel-coated balloons and pressure-augmented diffusion.
'OBJECTIVES. This study was designed to assess the feasibility of using hydrogel-coated balloons to deliver biologically active agents to the blood vessel wall. BACKGROUND. The local intramural delivery of therapeutic agents during balloon angioplasty has been proposed as an adjunctive technique for preventing early intracoronary thrombosis and late restenosis. METHODS. ... More
Investigation of the anticoagulant mechanisms of a covalent antithrombin-heparin complex.
AuthorsBerry L, Stafford A, Fredenburgh J, O'Brodovich H, Mitchell L, Weitz J, Andrew M, Chan AK
JournalJ Biol Chem
PubMed ID9856996
'Recently, we developed a covalent antithrombin-heparin complex (ATH) as a possible treatment for respiratory distress syndrome. ATH reacted rapidly with thrombin and efficiently catalyzed the inhibition of either thrombin or factor Xa by exogenous antithrombin. In order to investigate mechanisms for the conjugate''s unusual anticoagulant properties, changes in fluorescence due ... More
Heparin adheres to the damaged arterial wall and inhibits its thrombogenicity.
AuthorsAraki H, Muramoto J, Nishi K, Jougasaki M, Inoue M
JournalCirc Res
PubMed ID1499106
'Heparin binds to thrombogenic extracellular matrices as well as to smooth muscle cells of the vascular wall in vitro. The inhibitory effects of heparin on thrombogenicity of the damaged arterial wall were examined in vivo using small mesenteric arteries of rats and a video recording system attached to a microscope. ... More
The interaction of platelet factor 4 with heparins of different chain length.
AuthorsMarshall SE, Luscombe M, Pepper DS, Holbrook JJ
JournalBiochim Biophys Acta
PubMed ID6419785
'The interaction of platelet factor 4 with heparin of varying chain lengths has been investigated by labelling the heparin with fluorescein isothiocyanate and monitoring the change in anisotropy of fluorescence when the protein is added to a solution of the polysaccharide. The shape of the titration curve depends on the ... More
Heparin localization and fine structure regulate Burkitt's lymphoma growth.
AuthorsBerry D, Lynn DM, Berry E, Sasisekharan R, Langer R,
JournalBiochem Biophys Res Commun
PubMed ID16904641
'Burkitt''s lymphoma (BL) is a B-cell malignancy associated with the Epstein-Barr virus (EBV). Mounting evidence has implicated heparan sulfate proteoglycans and heparan sulfate-like glycosaminoglycans (HSGAGs) in the initiation, severity, and progression of the malignancy. The importance of HSGAGs in regulating BL cell growth was therefore examined. Extracellular exogenous heparin inhibited ... More
Lectins and heparin-binding features of human spermatozoa as analyzed by flow cytometry.
AuthorsVasquez JM, Magargee SF, Kunze E, Hammerstedt RH
JournalAm J Obstet Gynecol
PubMed ID2124086
'Sperm cell surface characteristics may serve as a useful diagnostic feature for the evaluation of seminal samples, but to date no useful analytical method(s) is available for their routine quantitative analysis. Fluorescein isothiocyanate-conjugated lectins, proteins with unique and specific saccharide-binding characteristics, and heparin are potentially useful markers for such an ... More
Preparation and properties of fluorescent glycosaminoglycuronans labeled with 5-aminofluorescein.
AuthorsOgamo A, Matsuzaki K, Uchiyama H, Nagasawa K
JournalCarbohydr Res
PubMed ID7116357
'The uronic acid residues of all known glycosaminoglycuronans reacted with 5-aminofluorescein to yield fluorescent glycosaminoglycuronan derivatives, which showed fluorescence characteristics identical to those of fluorescein or 5-acetamidofluorescein. The fluorescent products could be purified by chromatography on Octyl-Sepharose; three preparations of labeled chondroitin 6-sulfate having different degrees of substitution, and a ... More
Specific binding of the glycosaminoglycan 3H-heparin to bull, monkey, and rabbit spermatozoa in vitro.
'In Vitro binding and some binding parameters of the glycosaminoglycan heparin to viable epididymal or ejaculated bull spermatozoa, ejaculated rabbit spermatozoa, and frozen-thawed rhesus monkey spermatozoa were investigated. Nonspecific binding was affected only by the concentration of 3H-heparin, whereas specific binding was saturable, reversible, and dependent on the pH, temperature, ... More
Signal transduction and regulation in smooth muscle.
AuthorsSomlyo AP, Somlyo AV
JournalNature
PubMed ID7969467
'Smooth muscle cells in the walls of many organs are vital for most bodily functions, and their abnormalities contribute to a range of diseases. Although based on a sliding-filament mechanism similar to that of striated muscles, contraction of smooth muscle is regulated by pharmacomechanical as well as by electromechanical coupling ... More
Uptake of fluorescein isothiocyanate (FITC)-fractionated heparin by rat parenchymal hepatocytes in primary culture.
AuthorsWatanabe J, Muranishi H, Haba M, Yuasa H
JournalBiol Pharm Bull
PubMed ID8268865
The distribution of fractionated heparin in a primary culture of rat parenchymal hepatocytes was investigated optically using the fluorescence labelled drug and confocal imaging system with an inverted fluorescence microscope. The cell-associated fluorescein isothiocyanate (FITC)-fractionated heparin was observed to increase in conjunction with incubation time and also to localize, suggesting ... More
Transdermal delivery of heparin using pulsed current iontophoresis.
AuthorsPacini S, Punzi T, Gulisano M, Cecchi F, Vannucchi S, Ruggiero M,
JournalPharm Res
PubMed ID16362453
PURPOSE: In clinical practice heparin has to be administered by injection with obvious disadvantages; thus, transdermal delivery by electrically assisted methods have been studied. In this study we evaluated the efficacy of a Food and Drug Administration-approved pulsed current iontophoresis system in delivering heparin through living rat skin. METHODS: Fluorescent ... More
Matrix metalloproteinase processing of CXCL11/I-TAC results in loss of chemoattractant activity and altered glycosaminoglycan binding.
AuthorsCox JH, Dean RA, Roberts CR, Overall CM,
JournalJ Biol Chem
PubMed ID18411283
The CXCR3 chemokine receptor regulates the migration of Th1 lymphocytes and responds to three ligands: CXCL9/MIG, CXCL10/IP-10, and CXCL11/I-TAC. We screened for potential regulation of T cell responses by matrix metalloproteinase (MMP) processing of these important chemokines. The most potent of the CXCR3 ligands, CXCL11, was identified by matrix-assisted laser ... More
The interaction between heparin and antithrombin III: a comparison of two different heparin-dye conjugates.
AuthorsJones GR, Hashim R, Power DM
JournalBiochim Biophys Acta
PubMed ID3109497
The interactions of antithrombin III with two heparin-dye conjugates have been compared using their fluorescence anisotropy. The first, heparin labelled with 5-isothiocyanatofluorescein, where the dye was mostly bound to unsulphated glucosamine residues, exhibited binding which was characteristic of heparin with a low affinity for antithrombin III. The second, heparin labelled ... More
Determination of heparin-induced IgG antibody by fluorescence-linked immunofiltration assay (FLIFA).
AuthorsWang L, Huhle G, Malsch R, Hoffmann U, Song X, Harenberg J
JournalJ Immunol Methods
PubMed ID10022376
A fluorescence-linked immunofiltration assay (FLIFA) was developed for the determination of heparin-induced IgG in heparin-induced thrombocytopenia (HIT) type II patients. Protein A was immobilized on a nitrocellulose membrane to bind heparin-induced IgG of HIT type II patients. Fluorescein-5-isothiocynate (FITC)-heparin was added to platelet factor 4 present in normal serum to ... More
Preparation of biologically active fluorescent heparin composed of fluorescein-labeled species and its behavior to antithrombin III.
AuthorsUchiyama H, Nagasawa K
JournalJ Biochem (Tokyo)
PubMed ID6783633
A hog-mucosal heparin purified gel-chromatographically (total sulfate, 2.20; N-sulfate, 0.82; N-acetyl, 0.15 (mol/mol HexN); activity, 184 USP units/mg) was partially N-desulfated to give a product (total sulfate, 2.12; N-sulfate, 0.71 (mol/mol HexN); activity, 159 USP units/mg). The free amino groups in the product was extensively labeled with fluoresceinylthiocarbamoyl (FTC) groups, ... More
A comparative study of low-density lipoprotein interaction with glycosaminoglycans.
AuthorsGigli M, Ghiselli G, Torri G, Naggi A, Rizzo V
JournalBiochim Biophys Acta
PubMed ID8466951
The association between low-density lipoprotein (LDL) and a series of well characterized dermatan and chondroitin sulfates has been investigated by means of the fluorescence anisotropy technique with competition experiments using a fluorescein-labeled high LDL-affinity heparin fraction as a reference. Preparations of glycosaminoglycan (GAG) with sulfation degrees varying over a wide ... More
Preparation and properties of fluorescent polysaccharides.
AuthorsGlabe CG, Harty PK, Rosen SD
JournalAnal Biochem
PubMed ID6869815
A new method for preparing fluorescein derivatives of polysaccharides is described. These derivatives are prepared by activation of the polysaccharide with cyanogen bromide and subsequent reaction with fluoresceinamine. The optimum conditions for coupling have been established in this report. Using this procedure, we have prepared fluorescein derivatives of a wide ... More
Antiproliferative capacity of synthetic dextrans on smooth muscle cell growth: the model of derivatized dextrans as heparin-like polymers.
Proliferation of vascular smooth muscle cells (SMC) is postulated to be a key step in the pathogenesis of atherosclerosis or restenosis after vascular interventions such as angioplasty. Natural glycosaminoglycans, such as heparin and heparan sulfate, are known for their ability to inhibit SMC proliferation in vivo and in vitro. The ... More
Effect of heparin on the inhibition of factor Xa by tissue factor pathway inhibitor: a segment, Gly212-Phe243, of the third Kunitz domain is a heparin-binding site.
AuthorsEnjyoji K, Miyata T, Kamikubo Y, Kato H
JournalBiochemistry
PubMed ID7727433
Tissue factor pathway inhibitor (TFPI) inhibits the tissue factor--factor VIIa complex and factor Xa with its first and second Kunitz domains (K1 and K2), respectively. The inhibitory activity is enhanced by heparin, and the C-terminal basic part has been shown to be a heparin-binding site (HBS-1). To characterize and localize ... More
Specific binding of [3H]heparin to bovine granulosa cell membranes.
AuthorsBushmeyer SM, Bellin ME, Ax RL
JournalMol Cell Endocrinol
PubMed ID3840752
Bovine granulosa cell membranes from small (SFM) and large (LFM) antral follicles were incubated with [3H]heparin, a commercial radioactively labeled glycosaminoglycan (GAG). Binding was specific, reversible, saturable, and dependent on time, pH, ionic strength and divalent cations. SFM exhibited different [3H]heparin binding characteristics compared to LFM. The addition of a ... More
Heparin binding to human plasma low-density lipoproteins: dependence on heparin sulfation degree and chain length.
AuthorsGigli M, Consonni A, Ghiselli G, Rizzo V, Naggi A, Torri G
JournalBiochemistry
PubMed ID1627542
Binding between low-density lipoproteins (LDL) and fluorescein-labeled heparin was studied quantitatively with a modified form of a published procedure [Cardin, A. D., Randall, C. I., Hirose, N., & Jackson, R. L. (1987) Biochemistry 26, 5513-5518], using fluorescence anisotropy titrations. Assumption of binding site equivalence satisfactorily interpreted experimental data. Accordingly, the ... More
Evidence that binding to the carboxyl-terminal heparin-binding domain (Hep II) dominates the interaction between plasma fibronectin and heparin.
We assessed the participation of the three known heparin-binding domains of PFn (Hep I, Hep II, Hep III) in their interaction with heparin by making a quantitative comparison of the fluid-phase heparin affinities of PFn and PFn fragments under physiologic pH and ionic strength conditions. Using a fluorescence polarization binding ... More
Binding of heparin by type III domains and peptides from the carboxy terminal hep-2 region of fibronectin.
AuthorsIngham KC, Brew SA, Migliorini MM, Busby TF
JournalBiochemistry
PubMed ID8241146
The major sites of heparin binding by fibronectin are located in fragments of 30 or 40 kDa that contain type III modules 12 through 14 or 15. Various proteolytic or recombinant subfragments and several synthetic peptides derived from this region have been compared with respect to their binding to fluorescein-labeled ... More
The core of tau-paired helical filaments studied by scanning transmission electron microscopy and limited proteolysis.
Authorsvon Bergen M, Barghorn S, Müller SA, Pickhardt M, Biernat J, Mandelkow EM, Davies P, Aebi U, Mandelkow E
JournalBiochemistry
PubMed ID16700555
In Alzheimer's disease and frontotemporal dementias the microtubule-associated protein tau forms intracellular paired helical filaments (PHFs). The filaments formed in vivo consist mainly of full-length molecules of the six different isoforms present in adult brain. The substructure of the PHF core is still elusive. Here we applied scanning transmission electron ... More
A Toxoplasma lectin-like activity specific for sulfated polysaccharides is involved in host cell infection.
AuthorsOrtega-Barria E, Boothroyd JC
JournalJ Biol Chem
PubMed ID9880495
Toxoplasma gondii is one of the most widespread parasites of humans and animals. The parasite has a remarkable ability to invade a broad range of cells within its mammalian hosts by mechanisms that are poorly understood at the molecular level. This broad host cell specificity suggests that adhesion should involve ... More
Mechanism of acceleration of antithrombin-proteinase reactions by low affinity heparin. Role of the antithrombin binding pentasaccharide in heparin rate enhancement.
AuthorsStreusand VJ, Björk I, Gettins PG, Petitou M, Olson ST
JournalJ Biol Chem
PubMed ID7721817
The role of the sequence-specific pentasaccharide region of high affinity heparin (HAH) in heparin acceleration of antithrombin-proteinase reactions was elucidated by determining the accelerating mechanism of low affinity heparin (LAH) lacking this sequence. LAH was shown to be free of HAH (< 0.001%) from the lack of exchange of added ... More
Entry and distribution of fluorescent antiproliferative heparin derivatives into rat vascular smooth muscle cells: comparison between heparin-sensitive and heparin-resistant cultures.
AuthorsBârzu T, Pascal M, Maman M, Roque C, Lafont F, Rousselet A
JournalJ Cell Physiol
PubMed ID8698843
We studied the binding and entry of fluorescein (FITC)-labeled heparin derivatives into rat aortic smooth muscle cells (SMC) by confocal microscopy. FITC-labeled heparin fractions or FITC-labeled SR 80037A, a potent antiproliferative heparin derivative (Bârzu et al., Eur. J. Pharmacol., 219:225-233 1992), were prepared and their antiproliferative activity was confirmed. By ... More
Anticoagulants impact on innate immune responses and bacterial survival in whole blood models of Neisseria meningitidis infection.
Authors
JournalSci Rep
PubMed ID29977064
Endothelial cell glycocalyx modulates immobilization of leukocytes at the endothelial surface.
Authors
JournalArterioscler Thromb Vasc Biol
PubMed ID12855481
Targeting regulation of the tumour microenvironment induces apoptosis of breast cancer cells by an affinity hemoperfusion adsorbent.
Authors
JournalArtif Cells Nanomed Biotechnol
PubMed ID33754901
Sperm-Particle Interactions and Their Prospects for Charge Mapping.
Authors
JournalAdv Biosyst
PubMed ID32648653
RNA Binds to Tau Fibrils and Sustains Template-Assisted Growth.
Authors
JournalBiochemistry
PubMed ID26177386
Single Cell Transcriptomic Analyses Reveal the Impact of bHLH Factors on Human Retinal Organoid Development.
Authors
JournalFront Cell Dev Biol
PubMed ID34055784
Key steps in unconventional secretion of fibroblast growth factor 2 reconstituted with purified components.
Authors
JournalElife
PubMed ID28722655
Exploitation of a newly-identified entry pathway into the malaria parasite-infected erythrocyte to inhibit parasite egress.