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引用和文献 (10)
Invitrogen™
碘化乙啶
碘化乙啶是一种核酸染色剂，具有哺乳动物细胞膜通透性，可在革兰氏阳性菌环境下选择性地染色几乎所有革兰氏阳性菌。与 DNA 结合后，碘化乙啶会发出最大激发/发射波长为 ∼518/600 的荧光。通常，真核细胞的细胞质和细胞核都会被碘化乙啶染色。了解更多信息
| 货号 | 数量 |
|---|---|
| H7593 | 5 mg |
货号 H7593
价格（CNY)
4,497.00
Each
数量:
5 mg
价格（CNY)
4,497.00
Each
碘化乙啶是一种核酸染色剂，具有哺乳动物细胞膜通透性，可在革兰氏阳性菌环境下选择性地染色几乎所有革兰氏阳性菌。与 DNA 结合后，碘化乙啶会发出最大激发/发射波长为 ∼518/600 的荧光。通常，真核细胞的细胞质和细胞核都会被碘化乙啶染色。
仅供科研使用。不可用于诊断程序。
规格
颜色橙色
检测方法荧光
染料类型细胞通透性
激发波长范围518⁄600
适用于（设备）荧光显微镜
数量5 mg
运输条件室温
标签类型Fluorescent Dye
产品类型核酸染色剂
亚细胞定位细胞核、细胞质&细胞溶质, Cytosol, Nucleus
Unit SizeEach
内容与储存
室温避光储存。
引用和文献 (10)
引用和文献
Abstract
A flow-cytometric gram-staining technique for milk-associated bacteria.
Journal:Appl Environ Microbiol
PubMed ID:12732558
'A Gram-staining technique combining staining with two fluorescent stains, Oregon Green-conjugated wheat germ agglutinin (WGA) and hexidium iodide (HI) followed by flow-cytometric detection is described. WGA stains gram-positive bacteria while HI binds to the DNA of all bacteria after permeabilization by EDTA and incubation at 50 degrees C for 15
A fluorescent Gram stain for flow cytometry and epifluorescence microscopy.
Journal:Appl Environ Microbiol
PubMed ID:9647848
'The fluorescent nucleic acid binding dyes hexidium iodide (HI) and SYTO 13 were used in combination as a Gram stain for unfixed organisms in suspension. HI penetrated gram-positive but not gram-negative organisms, whereas SYTO 13 penetrated both. When the dyes were used together, gram-negative organisms were rendered green fluorescent by
Metabolic activity in filamentous fungi can be analysed by flow cytometry.
Journal:J Microbiol Methods
PubMed ID:12782375
The use of flow cytometry in combination with fluorescent dyes as a technique to rapidly differentiate and enumerate bacterial and yeast cells is well established. We have shown that through the judicial choice of stains, the nondestructive screening and sorting of fungal material is possible. The early stages of growth,
Increased sensitivity of bacterial detection in cerebrospinal fluid by fluorescent staining on low-fluorescence membrane filters.
Journal:J Med Microbiol
PubMed ID:16091435
A membrane-filter-based, fluorescent Gram stain method for bacterial detection in cerebrospinal fluid samples was developed and evaluated as a rapid, sensitive alternative to standard Gram stain protocols. A recently developed, modified version of the aluminium oxide membrane Anopore with low-fluorescence optical properties showed superior performance in this application. Other aspects
Nucleic acid stains as indicators of Cryptosporidium parvum oocyst viability.
Journal:Int J Parasitol
PubMed ID:9279581
We developed nucleic acid dye staining methodology for untreated, heat-treated and chemically inactivated C. parvum oocysts. The nucleic acid staining was compared to in vitro excystation and animal infectivity using split samples of oocysts. Among the nucleic acid stains tested, SYTO-9, hexidium and SYTO-59 stained the oocysts consistently, and the