人可铺板肝细胞,5-供体
Promotion on select lots. For lot selection and bulk orders contact Hepatic Products.
人可铺板肝细胞,5-供体
Gibco™

人可铺板肝细胞,5-供体

These '5-Donor' Human Plateable Hepatocytes are a pooled population of primary hepatocytes produced by combining cells from 5 individual donors了解更多信息
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货号 HMCPP5
价格(CNY)
30,395.00
Each
添加至购物车
价格(CNY)
30,395.00
Each
添加至购物车
These '5-Donor' Human Plateable Hepatocytes are a pooled population of primary hepatocytes produced by combining cells from 5 individual donors in a single vial. Pooling donors helps to minimize lot-to-lot variation and improves predictability of drug metabolism studies and other biological assays.

• Pure, highly viable, plateable hepatocytes
• Lot-specific characterization data
• Thawing and plating process compatible with existing protocols

Each lot is tested for:
• Attachment efficiency
• Monolayer formation and integrity
• Confluency >80% on Day 2
• Tested for plating in 96 well format
• Phase I enzyme activities
• Levels of CYP1A2, CYP2B6, and CYP3A4 induction (mRNA)
• Monolayer formation in medium with and without HepExtend (A2737501) supplement

Consistent cell attachment
High-quality plateable primary hepatocytes with high viabilities, proper cell morphology, and metabolic activity increase the ability to draw in vitro/in vivo correlations and make sound decisions regarding a compound’s fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

5 donors in a single vial
The cells are provided in a cryopreserved format similar to single donor plateable hepatocytes and have been shown to have approximately equal representation of each donor when plated.

Verified activity
Every lot of pooled plateable hepatocytes is tested for Phase I enzyme activity, cell viability, plating efficiency, and morphology.

For Research Use Only. Not for use in diagnostic procedures.
规格
池数量6 x 106
产品类型肝细胞
数量6 x 106 cells
运输条件仅限 LN2 干式运输罐
来源
年龄成人
细胞类型肝细胞
培养类型贴壁细胞培养
形式冻存
种属
组织
Unit SizeEach

常见问题解答 (FAQ)

Who should I contact if I am looking for hepatocytes with particular characteristics?

Our hepatocytes are prequalified and sold according to application, such as induction, short-term metabolism, and transporter uptake. If you require hepatocytes with particular P450 values or donor specifications, contact our Hepatic Biology Tech Support Team (hepaticproducts@thermofisher.com.com) or (866) 952-3559 to help with specific lot recommendations.

Who can I call if I have technical questions about hepatocyte cell culture?

Thermo Fisher Scientific has a dedicated team to answer questions regarding hepatocyte cell culture. You can send a question by email (hepaticproducts@thermofisher.com) or contact us by phone: US toll-free: (866) 952-3559

Are there guidelines for the procurement of human liver tissue?

Thermofisher Scientific works with a variety of human tissue sources, including Tissue and Organ Procurement Organizations, qualified Research Tissue Organizations and prominent academic and medical centers through collaborations that follow rigorous regulations, certifications and/or accreditations (“Source Facilities”). Tissues obtained through source facilities are consistent with the applicable legal and ethical practices of the United States.

Source Facilities are required by federal law to maintain accurate records and donor confidentiality as well as adhere to certain applicable laws governing tissue used for research globally. These include, but are not limited to:

- 45 CFR Part 46, subparts A, B, C, D where applicable (US federal guidance on the use of human subjects in research)
- Health Insurance Portability and Accountability Act (HIPAA) (addressing issues of confidentiality of personal information)
- National Organ Transplant Act (42 CFR Part 482)
- The Uniform Anatomical Gift Act (UAGA) of 1968, revised 1984

Where do you get your human liver tissue?

The human liver cells are derived from tissue obtained from accredited institutions. Consent is obtained by these institutions from the donor or the donor’s legal next of kin, for the use of the tissue and its derivatives for research purpose.

What should I plate hepatocyte cells on?

Hepatocytes need to be plated on collagen I-coated culture vessels.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.