可铺板培养人肝细胞，转运体测试合格

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Gibco™

可铺板培养人肝细胞，转运体测试合格

Name: 可铺板培养人肝细胞，转运体测试合格
SKU: HMCPTS
Price: 32001.00 CNY

在 4°C 和 37°C 下使用摄取转运体试验评估可铺板冻存人肝细胞的转运体功能。每个小瓶的细胞数：400-800 万将肝细胞培养物与牛磺胆酸盐和雌二醇 17β了解更多信息

货号 HMCPTS

价格（CNY)

32,001.00

Each

添加至购物车

价格（CNY)

32,001.00

Each

添加至购物车

在 4°C 和 37°C 下使用摄取转运体试验评估可铺板冻存人肝细胞的转运体功能。

每个小瓶的细胞数：400-800 万

将肝细胞培养物与牛磺胆酸盐和雌二醇 17β 葡萄糖苷酸 (E2-17G)（分别为摄取和外排转运体 NTCP/BSEP 和 OATP/MRP2 的底物）一起孵育。计算蓄积率和促进的活性成分摄取。

使用 GIBCO 肝细胞获得您需要的结果
高质量的肝细胞，具有高活力、适当的细胞形态和转运体功能，这都增强了判定体内⁄体外相关性的能力，并就化合物的结局做出合理的判定。经验丰富的技术人员、已获专利的分离技术、再辅以严格的放行标准，确保您获得最高品质的冻存肝细胞。

每批细胞均进行了以下测试：
• 存活率 ≥ 70%
• 贴壁率
• 单层形成能力和完整性
• 第 4 天时汇合率 >80%
• 以 96 孔规格进行铺板检测
• I 相酶活性
• 转运体活性
• 胆小管形成
• 相关 CYP450 多态性

相关链接：

请参阅了解更多关于我们的冻存肝细胞的信息
请参阅了解我们的全系列肝细胞的信息，

注：
这些产品需要用 LN₂ 蒸汽杜瓦瓶运输，因此可能需要额外收费。

For Research Use Only. Not for use in diagnostic procedures.

规格

供体单一供体

池数量4-8 x 10^6

产品线Gibco™

产品类型肝细胞

数量1 瓶

细胞系Primary

细胞类型肝细胞

培养类型肝细胞

形式冻存

种属人

组织Liver

Unit SizeEach

常见问题解答 (FAQ)

Who should I contact if I am looking for hepatocytes with particular characteristics?

Our hepatocytes are prequalified and sold according to application, such as induction, short-term metabolism, and transporter uptake. If you require hepatocytes with particular P450 values or donor specifications, contact our Hepatic Biology Tech Support Team (hepaticproducts@thermofisher.com.com) or (866) 952-3559 to help with specific lot recommendations.

Who can I call if I have technical questions about hepatocyte cell culture?

Thermo Fisher Scientific has a dedicated team to answer questions regarding hepatocyte cell culture. You can send a question by email (hepaticproducts@thermofisher.com) or contact us by phone: US toll-free: (866) 952-3559

Are there guidelines for the procurement of human liver tissue?

Thermofisher Scientific works with a variety of human tissue sources, including Tissue and Organ Procurement Organizations, qualified Research Tissue Organizations and prominent academic and medical centers through collaborations that follow rigorous regulations, certifications and/or accreditations (“Source Facilities”). Tissues obtained through source facilities are consistent with the applicable legal and ethical practices of the United States.

Source Facilities are required by federal law to maintain accurate records and donor confidentiality as well as adhere to certain applicable laws governing tissue used for research globally. These include, but are not limited to:

- 45 CFR Part 46, subparts A, B, C, D where applicable (US federal guidance on the use of human subjects in research)
- Health Insurance Portability and Accountability Act (HIPAA) (addressing issues of confidentiality of personal information)
- National Organ Transplant Act (42 CFR Part 482)
- The Uniform Anatomical Gift Act (UAGA) of 1968, revised 1984

Where do you get your human liver tissue?

The human liver cells are derived from tissue obtained from accredited institutions. Consent is obtained by these institutions from the donor or the donor’s legal next of kin, for the use of the tissue and its derivatives for research purpose.

What should I plate hepatocyte cells on?

Hepatocytes need to be plated on collagen I-coated culture vessels.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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