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View additional product information for Activated Human Myofibroblastic Hepatic Stellate Cells (MF-HSCs) - FAQs (HMFHSC)
12 product FAQs found
Our hepatocytes are prequalified and sold according to application, such as induction, short-term metabolism, and transporter uptake. If you require hepatocytes with particular P450 values or donor specifications, contact our Hepatic Biology Tech Support Team (hepaticproducts@thermofisher.com.com) or (866) 952-3559 to help with specific lot recommendations.
Thermo Fisher Scientific has a dedicated team to answer questions regarding hepatocyte cell culture. You can send a question by email (hepaticproducts@thermofisher.com) or contact us by phone: US toll-free: (866) 952-3559
Thermofisher Scientific works with a variety of human tissue sources, including Tissue and Organ Procurement Organizations, qualified Research Tissue Organizations and prominent academic and medical centers through collaborations that follow rigorous regulations, certifications and/or accreditations (“Source Facilities”). Tissues obtained through source facilities are consistent with the applicable legal and ethical practices of the United States.
Source Facilities are required by federal law to maintain accurate records and donor confidentiality as well as adhere to certain applicable laws governing tissue used for research globally. These include, but are not limited to:
- 45 CFR Part 46, subparts A, B, C, D where applicable (US federal guidance on the use of human subjects in research)
- Health Insurance Portability and Accountability Act (HIPAA) (addressing issues of confidentiality of personal information)
- National Organ Transplant Act (42 CFR Part 482)
- The Uniform Anatomical Gift Act (UAGA) of 1968, revised 1984
The human liver cells are derived from tissue obtained from accredited institutions. Consent is obtained by these institutions from the donor or the donor’s legal next of kin, for the use of the tissue and its derivatives for research purpose.
The qHSCs only go through a few passages, just sufficient for them to get activated. However, the low number of passages maintains their ability to get induced by inflammatory cytokines.
We do not offer quiescent human stellate cells (q-HSCs). However, we do offer Activated Human Myofibroblastic Hepatic Stellate Cells (MF-HSCs) (Cat. No. HMFHSC).
We have found that culturing hepatic stellate cells in collagen I-coated wells helps to maintain the morphology of the stellate cells.
Propagation of stellate cells results in further activation of the cells which significantly reduces the ability of the cells to get induced by inflammatory cytokines. Hence, we do not recommended further propagation of the cells.
The cells were treated with 2.5 ng/mL of TGF-β1 for 48 hr.
We have used a ratio between 10:1 and 3:1 for 3D-spheroid hepatocytes to stellate cells for a successful 3D co-culture. We would recommend testing different ratios as the cells may vary from one type of model to another.
Yes, we have done co-culturing of hepatic stellate cells along with 3D-spheroid hepatocytes.
Activated Human Myofibroblastic Hepatic Stellate Cells (MF-HSCs) are produced from isolated pure quiescent human stellate cells (q-HSCs). The cells are obtained from a single donor and provided in a cryopreserved format identical to single-donor plateable human hepatocytes. The q-HSCs are activated to become MF-HSCs through a process of passaging and maintenance in cell culture media. Activated MF-HSCs are suitable for building hepatic co-culture with primary hepatocytes to study in vitro disease models such as hepatic fibrosis.