Indo-1, Pentapotassium Salt, cell impermeant - FAQs

查看更多产品信息 Indo-1, Pentapotassium Salt, cell impermeant - FAQs (I1202)

2 个常见问题解答

使用流式细胞仪时，可以使用什么指示染料来测定钙离子流？

Indo-1，AM流式细胞仪的首选染料。它更适用于单激光激发（通常使用351-364 nm的氩离子激光的谱线），可监视两个发射。Indo-1的最大发射从在无Ca2+培养基中的〜475 nm变为Ca2+饱和的〜400 nm。Indo-1，AM是特别适用于多色荧光应用。

What indicator dye can I use to measure calcium flux by flow cytometry?

Indo-1, AM is the preferred stain for flow cytometry, where it is more practical to use a single laser for excitation, usually the 351–364 nm spectral lines of the argon-ion laser, and monitor two emissions. The emission maximum of Indo-1 shifts from ~475 nm in Ca2+-free medium to ~400 nm when the dye is saturated with Ca2+. Indo-1, AM is particularly suited for multicolor fluorescence applications.

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