JournalJournal of immunology (Baltimore, Md. : 1950)
PubMed ID10903717
CD5 antibodies increase intracellular ionized calcium concentration in T cells.
AuthorsJune CH,Rabinovitch PS,Ledbetter JA
JournalJournal of immunology (Baltimore, Md. : 1950)
PubMed ID3106489
The binding of a variety of monoclonal antibodies to the CD5 (T, gp67) pan T cell differentiation antigen has been shown to potentiate T cell proliferation. In this paper we show that CD5 monoclonal antibodies cause increased intracellular free calcium concentration ([Ca2+]i) in T cells. An increase in [Ca2+]i occurred ... More
The role of L-type Ca2+ current and Na+ current-stimulated Na/Ca exchange in triggering SR calcium release in guinea-pig cardiac ventricular myocytes.
AuthorsEvans AM, Cannell MB
JournalCardiovasc Res
PubMed ID9349392
OBJECTIVE: This study examines the relative ability of sodium current (INa)-stimulated reverse mode Na/Ca exchange and the L-type calcium current (ICa) to trigger calcium-induced calcium release (CICR) in guinea-pig ventricular myocytes. METHODS: Cytosolic Ca2+ transients were recorded from enzymatically dissociated guinea-pig ventricular myocytes using Indo-1. Macroscopic membrane currents were simultaneously ... More
Nitric oxide is an upstream signal of vascular endothelial growth factor-induced extracellular signal-regulated kinase1/2 activation in postcapillary endothelium.
AuthorsParenti A, Morbidelli L, Cui XL, Douglas JG, Hood JD, Granger HJ, Ledda F, Ziche M
JournalJ Biol Chem
PubMed ID9461619
We recently demonstrated that nitric oxide (NO) significantly contributes to the mitogenic effect of vascular endothelial growth factor (VEGF), suggesting a role for the NO pathway in the signaling cascade following kinase-derivative receptor activation in vascular endothelium. The aim of this study was to investigate the intracellular pathways linked to ... More
Cultured postnatal rat septohippocampal neurons change intracellular calcium in response to ethanol and nerve growth factor.
AuthorsWebb B, Suarez SS, Heaton MB, Walker DW
JournalBrain Res
PubMed ID9459553
Ethanol exposure affects cellular mechanisms involved in the regulation of calcium (Ca2+) homeostasis. Neurotrophins, such as nerve growth factor (NGF), stabilize intracellular Ca2+([Ca2+]i) during a variety of neurotoxic insults. In this study, changes in [Ca2+]i during treatment with ethanol and NGF were measured at the cell body of neurons using ... More
Characterization of cardiotin, a structural component in the myocard.
AuthorsSchaart G, van der Ven PF, Ramaekers FC
JournalEur J Cell Biol
PubMed ID8269977
The characterization and subcellular distribution of cardiotin, a structural component of striated muscle, is described using a monoclonal antibody. This high molecular mass component (> 300 kDa) is expressed in the myocard of several species and to a lesser extent also in skeletal muscle. Cardiotin is not found in smooth ... More
Activation of B- and T-cells by the cytoplasmic domains of the B-cell antigen receptor proteins Ig-alpha and Ig-beta.
In addition to membrane immunoglobulin (mIg), the B-cell antigen receptor contains Ig-alpha/Ig-beta heterodimers that link mIg to intracellular signaling molecules. To compare the ability of the cytoplasmic domains of Ig-alpha and Ig-beta to transduce signals in B- and T-cells, we constructed chimeric genes encoding the extracellular and transmembrane domains of ... More
Indo-1 binding to protein in permeabilized ventricular myocytes alters its spectral and Ca binding properties.
AuthorsHove-Madsen L, Bers DM
JournalBiophys J
PubMed ID1420876
We have examined the binding of the fluorescent Ca indicator indo-1 to cellular protein in permeabilized ventricular myocytes and also to soluble and particulate myocyte protein. Using either a filtration technique or equilibrium dialysis, and conditions similar to those in a cardiac myocyte patch clamped with 100 microM indo-1 in ... More
INCA: software for consort 30 analysis of flow cytometric calcium determinations.
AuthorsKeij JF, Griffioen AW, The TH, Rijkers GT
JournalCytometry
PubMed ID2582975
The INCA program converts Consort 30-generated fluorescence list mode data collected from Indo-1-stained cells to absolute intracellular calcium concentrations (nM Ca2+i). The calcium data are plotted vs. time, allowing the user to analyze the fractions of cells responding to a given stimulus. Converted files can be restored to disk after ... More
Thapsigargin, a tumor promoter, discharges intracellular Ca2+ stores by specific inhibition of the endoplasmic reticulum Ca2(+)-ATPase.
AuthorsThastrup O, Cullen PJ, Drøbak BK, Hanley MR, Dawson AP
JournalProc Natl Acad Sci U S A
PubMed ID2138778
Thapsigargin, a tumor-promoting sesquiterpene lactone, discharges intracellular Ca2+ in rat hepatocytes, as it does in many vertebrate cell types. It appears to act intracellularly, as incubation of isolated rat liver microsomes with thapsigargin induces a rapid, dose-dependent release of stored Ca2+. The thapsigargin-releasable pool of microsomal Ca2+ includes the pools ... More
Dual mechanisms of platelet hormone receptor desensitization. Differential importance between agonists of protein kinase C-dependent and -independent pathways.
AuthorsCrouch MF, Lapetina EG
JournalJ Biol Chem
PubMed ID2909542
Several different agonists, among them alpha-thrombin, platelet-activating factor, vasopressin, thromboxane A2, and endoperoxides, activate platelets to aggregate and secrete granular contents. Each of these agents is thought to act by inducing the turnover of inositol phospholipids and generating the second messenger molecules inositol 1,4,5-trisphosphate and 1,2-diacylglycerol. However, within minutes, the ... More
Identification of a signal transduction switch in the chemokine receptor CXCR1.
AuthorsSuetomi K, Rojo D, Navarro J
JournalJ Biol Chem
PubMed ID12077146
Chemokine receptors belong to the superfamily of G protein-coupled receptors, which regulate the trafficking and activation of leukocytes, and operate as coreceptors in the entry of HIV-1. To investigate the early steps in the signal transmission from the chemokine-binding site to the G protein-coupling region we engineered metal ion-binding sites ... More
Sarco/endoplasmic reticulum calcium ATPase-2 expression is regulated by ATF6 during the endoplasmic reticulum stress response: intracellular signaling of calcium stress in a cardiac myocyte model system.
AuthorsThuerauf DJ, Hoover H, Meller J, Hernandez J, Su L, Andrews C, Dillmann WH, McDonough PM, Glembotski CC
JournalJ Biol Chem
PubMed ID11595740
The recently described transcription factor, ATF6, mediates the expression of proteins that compensate for potentially stressful changes in the endoplasmic reticulum (ER), such as reduced ER calcium. In cardiac myocytes the maintenance of optimal calcium levels in the sarcoplasmic reticulum (SR), a specialized form of the ER, is required for ... More
Baseline cytosolic Ca2+ oscillations derived from a non-endoplasmic reticulum Ca2+ store.
AuthorsMissiaen L, Van Acker K, Parys JB, De Smedt H, Van Baelen K, Weidema AF, Vanoevelen J, Raeymaekers L, Renders J, Callewaert G, Rizzuto R, Wuytack F
JournalJ Biol Chem
PubMed ID11514551
'Cytosolic Ca(2+) oscillations can be due to cycles of release and re-uptake of internally stored Ca(2+). To investigate the nature of these Ca(2+) stores, we expressed the Pmr1 Ca(2+) pump of Caenorhabditis elegans in COS-1 cells and pretreated the cells with thapsigargin to prevent Ca(2+) uptake by the sarco(endo)plasmic reticulum ... More
N-formylpeptide-receptor dynamics, cytoskeletal activation, and intracellular calcium response in human neutrophil cytoplasts.
AuthorsOmann GM, Swann WN, Oades ZG, Parkos CA, Jesaitis AJ, Sklar LA
JournalJ Immunol
PubMed ID2824606
'Cytoplasts (enucleated neutrophils which are depleted of dense granules) were prepared from human neutrophils with a modified procedure which employed dihydrocytochalasin B instead of cytochalasin B. These cytoplasts retained an activatable cytoskeletal network similar to cells in that filamentous actin polymerization in response to an N-formylpeptide (fluoresceinated N-formyl-nle-leu-phe-nle-tyr-lys, FLPEP) occurred ... More
Triad formation: organization and function of the sarcoplasmic reticulum calcium release channel and triadin in normal and dysgenic muscle in vitro.
AuthorsFlucher BE, Andrews SB, Fleischer S, Marks AR, Caswell A, Powell JA
JournalJ Cell Biol
PubMed ID8245124
'Excitation-contraction (E-C) coupling is thought to involve close interactions between the calcium release channel (ryanodine receptor; RyR) of the sarcoplasmic reticulum (SR) and the dihydropyridine receptor (DHPR) alpha 1 subunit in the T-tubule membrane. Triadin, a 95-kD protein isolated from heavy SR, binds both the RyR and DHPR and may ... More
Anandamide activates vanilloid receptor 1 (VR1) at acidic pH in dorsal root ganglia neurons and cells ectopically expressing VR1.
AuthorsOlah Z, Karai L, Iadarola MJ
JournalJ Biol Chem
PubMed ID11333266
'The vanilloid receptor type 1 (VR1) is a heat-activated ionophore preferentially expressed in nociceptive neurons of trigeminal and dorsal root ganglia (DRG). VR1, which binds and is activated by capsaicin and other vanilloid compounds, was noted to interact with the endocannabinoid anandamide (ANA) and certain inflammatory metabolites of arachidonic acid ... More
Regulation of E-cadherin-mediated adhesion by muscarinic acetylcholine receptors in small cell lung carcinoma.
AuthorsWilliams CL, Hayes VY, Hummel AM, Tarara JE, Halsey TJ
JournalJ Cell Biol
PubMed ID8387530
'We present the first evidence that adhesion mediated by a member of the cadherin gene family can be regulated by a G protein-coupled receptor. We show that activating the M3 muscarinic acetylcholine receptor (mAChR) rapidly induces E-cadherin-mediated adhesion in a small cell lung carcinoma (SCLC) cell line. This response is ... More
Dispersion of cell-to-cell uncoupling precedes low K+-induced ventricular fibrillation.
AuthorsTribulová N, Manoach M, Varon D, Okruhlicová L, Zinman T, Shainberg A
JournalPhysiol Res
PubMed ID11521735
'We hypothesize that hypokalemia-related electrolyte imbalance linked with abnormal elevation of intracellular free Ca2+ concentration can cause metabolic disturbances and subcellular alterations resulting in intercellular uncoupling, which favor the occurrence of malignant arrhythmias. Langendorff-perfused guinea pig heart (n = 44) was subjected to a standard Tyrode solution (2.8 mmol/l K+) ... More
Inverse relationship of CA2+ mobilization and cell proliferation in CD8+ memory and virgin T cells.
'Memory T cells can now be defined with various monoclonal antibodies but little is known about the functional properties of these cells as compared to virgin T cells. We have studied Ca2+ mobilization and proliferation of memory and virgin cells in both the CD4 and CD8 subsets in response to ... More
An in vivo model for studying the dynamics of intracellular free calcium changes in slow- and fast-twitch muscle fibres.
AuthorsBátkai S, Rácz IB, Ivanics T, Tóth A, Hamar J, Slaaf DW, Reneman RS, Ligeti L
JournalPflugers Arch
PubMed ID10555564
'The understanding of the regulation of the free cytosolic [Ca2+] ([Ca2+]i) in skeletal muscle is hampered by the lack of techniques for quantifying free [Ca2+]i in muscle fibres in situ. We describe a model for studying the dynamics of free [Ca2+]i in the fast-twitch extensor digitorum longus (EDL) and the ... More
Metabolism and biologic effects of 5-oxoeicosanoids on human neutrophils.
AuthorsPowell WS, MacLeod RJ, Gravel S, Gravelle F, Bhakar A
JournalJ Immunol
PubMed ID8598482
'5-Oxo-6,8,11,14-eicosatetraenoic acid (5-oxo-ETE) is a recently discovered metabolite of arachidonic acid that activates human neutrophils by a mechanism independent of the receptor for leukotriene B4 (LTB4). The objectives of this study were to identify the major metabolites of 5-oxo-ETE in neutrophils and to compare the biologic activities of 5-oxo-ETE with ... More
Formation of 14,15-hepoxilins of the A(3) and B(3) series through a 15-lipoxygenase and hydroperoxide isomerase present in garlic roots.
AuthorsReynaud D, Ali M, Demin P, Pace-Asciak CR
JournalJ Biol Chem
PubMed ID10497175
'We report herein for the first time the formation by freshly grown garlic roots and the structural characterization of 14,15-epoxide positional analogs of the hepoxilins formed via the 15-lipoxygenase-induced oxygenation of arachidonic acid. These compounds are formed through the combined actions of a 15(S)-lipoxygenase and a hydroperoxyeicosatetraenoic acid (HPETE) isomerase. ... More
The effect of sulfated polysaccharides on the free intracellular calcium ion concentration of lymphocytes.
AuthorsTellam RL, Parish CR
JournalBiochim Biophys Acta
PubMed ID2441758
'Recent studies have demonstrated that murine lymphocytes express specific cell-surface receptors for a range of sulfated polysaccharides. In order to determine whether polysaccharide binding induces transmembrane signaling, the effects of sulfated polysaccharides on the free intracellular calcium ion concentration [( Ca2+]i) of mouse thymocytes and spleen cells were determined. Cells ... More
Long-term regulation of neuronal calcium currents by prolonged changes of membrane potential.
AuthorsFranklin JL, Fickbohm DJ, Willard AL
JournalJ Neurosci
PubMed ID1315850
'Although rapid-onset, short-term regulation of neuronal Ca currents by neurotransmitters and second messengers is well documented, little is known about conditions that can cause longer-lasting changes in Ca channel function. We report here that persistent depolarization is accompanied by slowly developing long-term reduction of neuronal Ca currents. Rat myenteric neurons ... More
Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors: evidence for two closely linked C-C chemokine receptors on chromosome 9.
Authors Boring L; Gosling J; Monteclaro F S; Lusis A J; Tsou C L; Charo I F;
JournalJ Biol Chem
PubMed ID8631787
'We have isolated cDNA clones that encode two closely related, murine C-C chemokine receptors. Both receptors are members of the G-protein-coupled, seven-transmembrane domain family of receptors and are most closely related to the human monocyte chemoattractant protein 1 receptor. Expression of each of the receptors was detected in murine monocyte/macrophage ... More
Mitochondrial modulation of Ca2+ -induced Ca2+ -release in rat sensory neurons.
AuthorsJackson JG, Thayer SA
JournalJ Neurophysiol
PubMed ID16760347
'Ca2+ -induced Ca2+ -release (CICR) from ryanodine-sensitive Ca2+ stores provides a mechanism to amplify and propagate a transient increase in intracellular calcium concentration ([Ca2+]i). A subset of rat dorsal root ganglion neurons in culture exhibited regenerative CICR when sensitized by caffeine. [Ca2+]i oscillated in the maintained presence of 5 mM ... More
The role of sarcolemmal Ca2+-ATPase in the regulation of resting calcium concentration in rat ventricular myocytes.
AuthorsChoi HS, Eisner DA
JournalJ Physiol
PubMed ID9925882
'1. The aim of this work was to investigate the role of sarcolemmal Ca2+-ATPase in rat ventricular myocytes. We have measured intracellular Ca2+ concentration ([Ca2+]i) using indo-1. The actions of the ATPase inhibitor carboxyeosin were studied. 2. Carboxyeosin increased resting [Ca2+]i and the magnitude of the systolic Ca2+ transient and ... More
Protective effects of low and high doses of cyclosporin A against reoxygenation injury in isolated rat cardiomyocytes are associated with differential effects on mitochondrial calcium levels.
AuthorsGriffiths EJ, Ocampo CJ, Savage JS, Stern MD, Silverman HS
JournalCell Calcium
PubMed ID10756975
'In this study we aimed to determine the concentration range of cyclosporin A (CsA) which was effective in protecting against reoxygenation injury in isolated cardiomyocytes, and its effects on intramitochondrial free calcium levels ([Ca2+]m). We also determined whether a high [CsA] had any deleterious effect on normal myocyte function. Isolated ... More
Regulation of Ca2+ influx during mitosis: Ca2+ influx and depletion of intracellular Ca2+ stores are coupled in interphase but not mitosis.
AuthorsPreston SF, Sha'afi RI, Berlin RD
JournalCell Regul
PubMed ID1809398
'Activation of a wide variety of membrane receptors leads to a sustained elevation of intracellular Ca2+ ([Ca2+]i) that is pivotal to subsequent cell responses. In general, in nonexcitable cells this elevation of [Ca2+]i results from two sources: an initial release of Ca2+ from intracellular stores followed by an influx of ... More
Detection of La3+ influx in ventricular cells by indo-1 fluorescence.
AuthorsPeeters GA, Kohmoto O, Barry WH
JournalAm J Physiol
PubMed ID2919662
'We exposed indo-1-loaded cultured embryonic chick ventricular cells to 0.03-1.0 mM extracellular lanthanum concentration ([La3+]o) and simultaneously measured cell contractile motion and the 410/480 nm fluorescence intensity ratio. After exposure to La3+, ventricular cells stopped contracting and relaxed within seconds, and the 410/480 fluorescence ratio increased. The increase in the ... More
The cytoplasmic domain of human FcgammaRIa alters the functional properties of the FcgammaRI.gamma-chain receptor complex.
'The gamma/zeta-chain family of proteins mediate cell activation for multiple immunoglobulin receptors. However, the recognition that these receptors may have distinct biologic functions suggests that additional signaling elements may contribute to functional diversity. We hypothesized that the cytoplasmic domain (CY) of the ligand binding alpha-chain alters the biological properties of ... More
Oxygen-bridged dinuclear ruthenium amine complex specifically inhibits Ca2+ uptake into mitochondria in vitro and in situ in single cardiac myocytes.
AuthorsMatlib MA, Zhou Z, Knight S, Ahmed S, Choi KM, Krause-Bauer J, Phillips R, Altschuld R, Katsube Y, Sperelakis N, Bers DM
JournalJ Biol Chem
PubMed ID9553073
'Ruthenium red is a well known inhibitor of Ca2+ uptake into mitochondria in vitro. However, its utility as an inhibitor of Ca2+ uptake into mitochondria in vivo or in situ in intact cells is limited because of its inhibitory effects on sarcoplasmic reticulum Ca2+ release channel and other cellular processes. ... More
Membrane potential modulates release of tumor necrosis factor in lipopolysaccharide-stimulated mouse macrophages.
AuthorsHaslberger A, Romanin C, Koerber R
JournalMol Biol Cell
PubMed ID1498365
'Lipopolysaccharide (LPS)-mediated synthesis of macrophage gene products such as tumor necrosis factor (TNF) is controlled by different signaling pathways. We investigated intracellular free Ca2+ (Ca2+ic) and the membrane potential as early cellular responses to LPS and their role in the synthesis and release of TNF. In peritoneal macrophages and in ... More
Beta-adrenergic modulation of the polymorphonuclear leukocyte respiratory burst is dependent upon the mechanism of cell activation.
AuthorsNielson CP
JournalJ Immunol
PubMed ID2821113
'Although inhibition of polymorphonuclear leukocyte activation by beta-adrenoceptor agonists has been recognized for over a decade, effects have only been observed at high drug concentrations and in the presence of theophylline. In this study, catecholamine and prostaglandin modulation of the respiratory burst was evaluated with respect to the mechanism of ... More
Tyrphostin A9 inhibits calcium release-dependent phosphorylations and calcium entry via calcium release-activated channel in Jurkat T cells.
'The mechanism by which calcium-depleted intracellular stores may trigger an external calcium influx through a calcium release-activated channel was investigated by analyzing the effects of several protein tyrosine kinase inhibitors on calcium movements in Jurkat T cells. Tyrphostin A9, an inhibitor of the kinase activity of the platelet-derived growth factor ... More
Mechanisms for activation and subsequent removal of cytosolic Ca2+ in bradykinin-stimulated neuronal and glial cell lines.
AuthorsReiser G, Binmöller FJ, Donié F
JournalExp Cell Res
PubMed ID2298236
'Mechanisms for activation and for removal of cytosolic Ca2+ after stimulation with bradykinin were investigated in two neural cell lines by measuring cytosolic Ca2+ activity and 45Ca2+ fluxes. In the neuronal (neuroblastoma x glioma hybrid) and in the glial (rat glioma) cell lines, the transient, bradykinin-induced rise in cytosolic Ca2+ ... More
Cytoplasmic Ca2+ oscillations in pancreatic beta-cells.
AuthorsHellman B, Gylfe E, Grapengiesser E, Lund PE, Berts A
JournalBiochim Biophys Acta
PubMed ID1450203
'In the last 15 years it has been a growing interest in the cyclic variations of circulating insulin [46]. After the suggestion that this phenomenon may be due to oscillations of the beta-cell membrane potential [8,39], it was demonstrated that [Ca2+]i oscillates in the glucose-stimulated beta-cell with a similar frequency ... More
Anionic phospholipids stimulate an arachidonoyl-hydrolyzing phospholipase A2 from macrophages and reduce the calcium requirement for activity.
AuthorsLeslie CC, Channon JY
JournalBiochim Biophys Acta
PubMed ID2167132
'Mechanisms involved in regulating the activity of intracellular phospholipase A2 enzymes that function in eicosanoid and platelet-activating factor production are poorly understood. The properties of the substrate in the membrane may play a role in modulating phospholipase A2 activity. In this study, the effect of anionic phospholipids, diacylglycerol (DAG) and ... More
Time dependence of transmembrane potential changes and intracellular calcium flux in stimulated human monocytes.
AuthorsBernardo J, Brink HF, Simons ER
JournalJ Cell Physiol
PubMed ID2826501
'An important characteristic of the functional differentiation of the blood monocyte is the development of its capacity to recognize and respond to stimuli. This ability is mediated to a large extent by specific receptor glycoproteins located on the cell surface. Stimulation of mononuclear phagocytes via these receptors results in a ... More
Flow cytometric measurements of cytoplasmic calcium changes in human platelets.
AuthorsDavies TA, Drotts D, Weil GJ, Simons ER
JournalCytometry
PubMed ID3359892
'Thrombin-induced stimulation of human platelets is accompanied by a dramatic increase in cytoplasmic calcium concentrations followed by a slow decrease. These changes are very rapid, are maximal by 10-15 s, and can be detected with probes such as Indo-1. Suspension studies using spectrofluorometry, which reflect a value which is the ... More
Mitochondrial control of calcium-channel gating: a mechanism for sustained signaling and transcriptional activation in T lymphocytes.
AuthorsHoth M, Button DC, Lewis RS
JournalProc Natl Acad Sci U S A
PubMed ID10973476
'In addition to their well-known functions in cellular energy transduction, mitochondria play an important role in modulating the amplitude and time course of intracellular Ca(2+) signals. In many cells, mitochondria act as Ca(2+) buffers by taking up and releasing Ca(2+), but this simple buffering action by itself often cannot explain ... More
Hydrolysis of Ca2+-sensitive fluorescent probes by perfused rat heart.
AuthorsScaduto RC, Grotyohann LW
JournalAm J Physiol Heart Circ Physiol
PubMed ID14561682
'Rat hearts were loaded with the fluorescent calcium indicators fura 2, indo 1, rhod 2, or fluo 3 to determine cytosolic calcium levels in the perfused rat heart. With fura 2, however, basal tissue fluorescence increased above anticipated levels, suggesting accumulation of intermediates of fura 2-AM deesterification. To examine this ... More
Calcitonin stimulates growth of human prostate cancer cells through receptor-mediated increase in cyclic adenosine 3',5'-monophosphates and cytoplasmic Ca2+ transients.
'Our recent study has shown that a calcitonin (CT)-like immunoreactive substance(s) is secreted by cultured prostate cells, and secretion of this material is significantly higher in malignant than in benign prostate cells. To test the hypothesis that prostatic CT may serve as a paracrine/neuroendocrine factor, the present study investigated for ... More
Prostacyclin is a specific effector of adipose cell differentiation. Its dual role as a cAMP- and Ca(2+)-elevating agent.
AuthorsVassaux G, Gaillard D, Ailhaud G, Négrel R
JournalJ Biol Chem
PubMed ID1317853
'The mitogenic-adipogenic activity of carbaprostacyclin (cPGI2), a stable analogue of prostacyclin (PGI2), has been proposed to be related to its ability to elicit cAMP production and to activate the protein kinase A cascade (Négrel, R., Gaillard, D., and Ailhaud, G. (1989) Biochem. J. 257, 399-405). In the present study, cPGI2 ... More
Intracellular Ca2+ and delay of ischemia-induced electrical uncoupling in preconditioned rabbit ventricular myocardium.
AuthorsDekker LR, Coronel R, VanBavel E, Spaan JA, Opthof T
JournalCardiovasc Res
PubMed ID10615394
'OBJECTIVE: Short periods of ischemia and reperfusion alter myocardial Ca2+ handling and temporarily induce a mild increase of [Ca2+]i. We hypothesized that these alterations are involved in the cardioprotective mechanism of ischemic preconditioning, possibly via a Ca(2+)-dependent activation of protein kinase C (PKC). METHODS AND RESULTS: In arterially perfused rabbit ... More
Concomitant accumulation of intracellular free calcium and arachidonic acid in the ischemic-reperfused rat heart.
AuthorsIvanics T, Miklós Z, Dézsi L, Ikrényi K, Tóth A, Roemen TH, Van der Vusse GJ, Ligeti L
JournalMol Cell Biochem
PubMed ID11768232
'This study was designed to elucidate the relationship between enhanced cytoplasmic calcium levels (Ca2+i) and membrane phospholipid degradation, a key step in the loss of cellular integrity during cardiac ischemia/reperfusion-induced damage. Isolated rat hearts were subjected to 15 min ischemia followed by 30 min reperfusion. Ca2+i was estimated by the ... More
Actin assembly in electropermeabilized neutrophils: role of intracellular calcium.
AuthorsDowney GP, Chan CK, Trudel S, Grinstein S
JournalJ Cell Biol
PubMed ID2112547
'Assembly of microfilaments involves the conversion of actin from the monomeric (G) to the filamentous (F) form. The exact sequence of events responsible for this conversion is yet to be defined and, in particular, the role of calcium remains unclear. Intact and electropermeabilized human neutrophils were used to assess more ... More
Ouabain interaction with cardiac Na+/K+-ATPase initiates signal cascades independent of changes in intracellular Na+ and Ca2+ concentrations.
AuthorsLiu J, Tian J, Haas M, Shapiro JI, Askari A, Xie Z
JournalJ Biol Chem
PubMed ID10874029
'We have shown previously that partial inhibition of the cardiac myocyte Na(+)/K(+)-ATPase activates signal pathways that regulate myocyte growth and growth-related genes and that increases in intracellular Ca(2+) concentration ([Ca(2+)](i)) and reactive oxygen species (ROS) are two essential second messengers within these pathways. The aim of this work was to ... More
CD19 monoclonal antibody HD37 inhibits anti-immunoglobulin-induced B cell activation and proliferation.
AuthorsPezzutto A, Dörken B, Rabinovitch PS, Ledbetter JA, Moldenhauer G, Clark EA
JournalJ Immunol
PubMed ID2437199
'The 95 Kd CD19 antigen is the broadest lineage specific surface marker for B cells: it is present on the surface of virtually all B lymphocytes, including early B progenitor cells. In this study we have evaluated the function of the CD19 antigen by using the CD19 mAb HD37. Binding ... More
Ca2+ mobilization primes protein kinase C in human platelets. Ca2+ and phorbol esters stimulate platelet aggregation and secretion synergistically through protein kinase C.
AuthorsSiess W, Lapetina EG
JournalBiochem J
PubMed ID3143357
'Low concentrations of Ca2+-mobilizing agonists such as vasopressin, platelet-activating factor, ADP, the endoperoxide analogue U44069 and the Ca2+ ionophore A23187 enhance the binding of [3H]phorbol 12,13-dibutyrate (PdBu) to intact human platelets. This effect is prevented by preincubation of platelets with prostacyclin (except for A23187). Adrenaline, which does not increase Ca2+ ... More
The negative inotropic effect of beta3-adrenoceptor stimulation in the beating guinea pig heart.
AuthorsKitamura T, Onishi K, Dohi K, Okinaka T, Isaka N, Nakano T
JournalJ Cardiovasc Pharmacol
PubMed ID10813382
'Although beta3-adrenoceptors (ARs) have been extensively characterized in brown and white adipocytes, their actions in the beating heart are unclear. We examined the effects of a beta3-AR agonist, BRL37344, on cardiac function and calcium transients in Langendorff-perfused guinea pig hearts by simultaneously measuring left ventricular (LV) pressure and Ca2+-dependent indo-1 ... More
Intracellular free Ca2+ in the cell cycle in human fibroblasts: transitions between G1 and G0 and progression into S phase.
AuthorsWahl M, Gruenstein E
JournalMol Biol Cell
PubMed ID8485320
'Intracellular free calcium ([Ca2+]i) has been proposed to play an important part in the regulation of the cell cycle. Although a number of studies have shown that stimulation of quiescent cells with growth factors causes an immediate rise in [Ca2+]i (Rabinovitch et al., 1986; Vincentini and Villereal, 1986; Hesketh et ... More
Endothelin stimulation of cytosolic calcium and gonadotropin secretion in anterior pituitary cells.
'The presence of endothelin, a vasoconstrictor peptide, in the hypothalamus and posterior pituitary suggests that it also regulates neural and other nonvascular target cells. In pituitary gonadotrophs, low doses of endothelin evoked oscillations in the intracellular calcium concentration, and high doses induced a biphasic calcium response. Mobilization of intracellular calcium ... More
In vitro status epilepticus causes sustained elevation of intracellular calcium levels in hippocampal neurons.
'Calcium ions and calcium-dependent systems have been implicated in the pathophysiology of status epilepticus (SE). However, the dynamics of intracellular calcium ([Ca2+]i) levels during SE has not yet been studied. We have employed the hippocampal neuronal culture (HNC) model of in vitro SE that produces continuous epileptiform discharges to study ... More
Antibody binding to CD5 (Tp67) and Tp44 T cell surface molecules: effects on cyclic nucleotides, cytoplasmic free calcium, and cAMP-mediated suppression.
AuthorsLedbetter JA, Parsons M, Martin PJ, Hansen JA, Rabinovitch PS, June CH
JournalJ Immunol
PubMed ID3021852
'T cells can be activated to proliferate by antibodies to the T cell antigen receptor or the molecularly associated CD3 complex if monocytes are present. We have shown previously that monoclonal antibodies to the human T cell differentiation antigens CD5 (Tp67) and Tp44 each augment and prolong proliferative responses of ... More
Augmentation of ultraviolet B radiation-induced tumor necrosis factor production by the epidermal platelet-activating factor receptor.
AuthorsDy LC, Pei Y, Travers JB
JournalJ Biol Chem
PubMed ID10480902
'Ultraviolet B radiation (UVB) has been shown to damage human keratinocytes in part by inducing oxidative stress and cytokine production. Indeed, UVB-induced production of the pro-inflammatory and cytotoxic cytokine tumor necrosis factor alpha (TNF-alpha) has been implicated in the epidermal damage seen in response to acute solar radiation. Though the ... More
Sperm calcium levels and chlortetracycline fluorescence patterns are related to the in vivo fertility of cryopreserved bovine semen.
AuthorsCollin S, Sirard MA, Dufour M, Bailey JL
JournalJ Androl
PubMed ID11105921
'Cryopreserved bovine semen is less fertile than fresh semen for reasons that have not been fully elucidated. Cryopreservation is known to disrupt the sperm plasma membrane and it induces premature capacitation of a sperm subpopulation, which may be a result of the increased internal calcium levels after thawing. To test ... More
SLAT regulates Th1 and Th2 inflammatory responses by controlling Ca2+/NFAT signaling.
AuthorsBécart S, Charvet C, Canonigo Balancio AJ, De Trez C, Tanaka Y, Duan W, Ware C, Croft M, Altman A
JournalJ Clin Invest
PubMed ID17657315
'SWAP-70-like adapter of T cells (SLAT) is a novel guanine nucleotide exchange factor for Rho GTPases that is upregulated in Th2 cells, but whose physiological function is unclear. We show that SLAT(-/-) mice displayed a developmental defect at one of the earliest stages of thymocyte differentiation, the double-negative 1 (DN1) ... More
Inhibition of lysophosphatidate- and thrombin-induced neurite retraction and neuronal cell rounding by ADP ribosylation of the small GTP-binding protein Rho.
AuthorsJalink K, van Corven EJ, Hengeveld T, Morii N, Narumiya S, Moolenaar WH
JournalJ Cell Biol
PubMed ID8045941
'Addition of the bioactive phospholipid lysophosphatidic acid (LPA) or a thrombin receptor-activating peptide (TRP) to serum-starved N1E-115 or NG108-15 neuronal cells causes rapid growth cone collapse, neurite retraction, and transient rounding of the cell body. These shape changes appear to be driven by receptor-mediated contraction of the cortical actomyosin system ... More
Mechanism for depressed cardiac function in left ventricular volume overload.
'To assess the effects of left ventricular chamber volume on the mechanism of changes in left ventricular developed pressure we performed phosphorous-31 nuclear magnetic resonance spectroscopy, hydrogen-1 nuclear magnetic resonance spectroscopy with a shift reagent, two-dimensional echocardiography, atomic absorption spectrophotometry, microsphere analysis, and surface fluorometry on isovolumic isolated perfused rat ... More
Activity of an intracellular lymphocyte stimulator is independent of G-protein interactions, [Ca2+]i elevation, phosphoinositide hydrolysis, and protein kinase C translocation.
'We have evaluated potential molecular mechanisms by which a group of synthetic lymphokine-like molecules, the 7,8-disubstituted guanine ribonucleosides, acts on second messenger pathways to augment the responses of murine B lymphocytes. Despite its extensive structural homology with GTP, 7-methyl-8-oxoguanosine (7-Me-8-oGuo), a prototypical disubstituted nucleoside, does not inhibit the binding of ... More
Stimulus responses and amyloid precursor protein processing in DAMI megakaryocytes.
AuthorsDavies TA, Billingslea A, Johnson R, Greenberg S, Ortiz M, Long H, Sgro K, Tibbles H, Seetoo K, Rathbun W, Schonhorn J, Simons ER
JournalJ Lab Clin Med
PubMed ID9242363
'Platelets, when released as anuclear cells by their precursor megakaryocytes, already carry soluble proteolytic fragments of the amyloid precursor protein (APP) within their alpha-granules and intact APP in the alpha-granule membranes. In response to activation signals elicited by physiologic stimuli such as thrombin, platelets release their granules'' soluble contents and ... More
Abnormalities of cytoplasmic Ca2+ in platelets from patients with uremia.
AuthorsWare JA, Clark BA, Smith M, Salzman EW
JournalBlood
PubMed ID2910357
'Uremic patients have a hemorrhagic tendency, often associated with prolonged bleeding times and decreased platelet function in vitro. Whether these defects result from abnormalities in plasma factors affecting platelet activity, platelet surface receptors, intracellular platelet mediators, or other aspects of platelet behavior is unknown. To examine the possibility that the ... More
Anoxic contractile failure in rat heart myocytes is caused by failure of intracellular calcium release due to alteration of the action potential.
'Anoxia of the heart causes failure of contraction before any irreversible injury occurs; the mechanism by which anoxia blocks cardiac excitation-contraction coupling is unknown. Studies in whole muscle are confounded by heterogeneity; however, achieving the low oxygen tensions required to study anoxia in a single myocyte during electrophysiological recording has ... More
Stimulus-dependent modulation of smooth muscle intracellular calcium and force by altered intracellular pH.
AuthorsTaggart MJ, Burdyga T, Heaton R, Wray S
JournalPflugers Arch
PubMed ID8772130
'Measurements of simultaneous force and intracellular Ca2+ concentration ([Ca2+]i) in rat uterine smooth muscle have been made to elucidate the mechanisms involved when force produced spontaneously, by high-K+ depolarization or carbachol is altered by a change of intracellular pH (pHi). Rises in force and [Ca2+]i were closely correlated for all ... More
Differential regulation of Ca2+ mobilization in human thymocytes by coaggregation of surface molecules.
AuthorsDeusch K, Daley JF, Levine H, Languet AJ, Anderson P, Schlossman SF, Blue ML
JournalJ Immunol
PubMed ID1691220
'Variations in intracellular Ca2+ levels in developing thymocytes are likely to play a major role in both the activation-associated differentiation of thymocytes and in the selection or clonal deletion of cells. Here we examine the role of CD4, CD8, CD2, and CD45 in the regulation of intracellular Ca2+ levels in ... More
Caffeine inhibits the agonist-evoked cytosolic Ca2+ signal in mouse pancreatic acinar cells by blocking inositol trisphosphate production.
AuthorsToescu EC, O'Neill SC, Petersen OH, Eisner DA
JournalJ Biol Chem
PubMed ID1429689
'The inhibitory effects of caffeine on receptor-activated cytosolic Ca2+ signal generation in isolated mouse pancreatic acinar cells were investigated. Using the ability of caffeine to quench Indo-1 fluorescence we measured simultaneously the free intracellular Ca2+ concentration ([Ca2+]i) and the intracellular caffeine concentration ([caffeine]i). We also measured inositol 1,4,5-trisphosphate (InsP3) production ... More
Stimulation of T cells through the CD3/T-cell receptor complex: role of cytoplasmic calcium, protein kinase C translocation, and phosphorylation of pp60c-src in the activation pathway.
AuthorsLedbetter JA, Gentry LE, June CH, Rabinovitch PS, Purchio AF
JournalMol Cell Biol
PubMed ID2434833
'Stimulation of T cells or the Jurkat T-cell line with soluble antibodies to the CD3/T-cell receptor complex causes mobilization of cytoplasmic Ca2+, which is blocked by pertussis toxin but not by ethylene glycol-bis(beta-aminoethyl ether)-N,N,N'',N''-tetraacetic acid, and translocation of protein kinase C activity from the cytoplasm to the membrane. Such stimulation ... More
Ischemia/reperfusion-induced changes in intracellular free Ca2+ levels in rat skeletal muscle fibers--an in vivo study.
AuthorsIvanics T, Miklós Z, Ruttner Z, Bátkai S, Slaaf DW, Reneman RS, Tóth A, Ligeti L
JournalPflugers Arch
PubMed ID10898531
'Accumulation of intracellular free calcium (Ca2+i) may play an essential role in the ischemia/reperfusion injury of skeletal muscle. Although it has been shown that Ca2+i levels significantly increase during ischemia/reperfusion, it is still a matter of debate whether Ca2+i increases during ischemia alone. It was the aim of this study ... More
Isoleukotrienes are biologically active free radical products of lipid peroxidation.
AuthorsHarrison KA, Murphy RC
JournalJ Biol Chem
PubMed ID7615527
'The free radical oxidation of arachidonic acid esterified to glycerophospholipids is known to generate complex metabolites, termed isoprostanes, that share structural features of prostaglandins derived from prostaglandin H2 synthase. Furthermore, certain isoprostanes have been found to exert biological activity through endogenous receptors on cell surfaces. Using mass spectrometry and ancillary ... More
Prostacyclin inhibits platelet aggregation induced by phorbol ester or Ca2+ ionophore at steps distal to activation of protein kinase C and Ca2+-dependent protein kinases.
AuthorsSiess W, Lapetina EG
JournalBiochem J
PubMed ID2494992
'Suspensions of aspirin-treated, 32P-prelabelled, washed platelets containing ADP scavengers in the buffer were activated with either phorbol 12,13-dibutyrate (PdBu) or the Ca2+ ionophore A23187. High concentrations of PdBu (greater than or equal to 50 nM) induced platelet aggregation and the protein kinase C (PKC)-dependent phosphorylation of proteins with molecular masses ... More
Glutamate-loading stimulates metabolic flux and improves cell recovery following chemical hypoxia in isolated cardiomyocytes.
AuthorsWilliams H, King N, Griffiths EJ, Suleiman MS
JournalJ Mol Cell Cardiol
PubMed ID11735258
'The amino acid glutamate is used in cardioplegic solutions, yet evidence is conflicting as to whether or not exogenous glutamate is indeed cardioprotective. This controversy may be because increasing extracellular glutamate does not necessarily lead to an increase in intracellular glutamate. In this study we aimed to determine whether isolation ... More
GnRH-induced cytosolic calcium oscillations in pituitary gonadotrophs: phase resetting by membrane depolarization.
AuthorsVergara LA, Stojilkovic SS, Rojas E
JournalBiophys J
PubMed ID8534831
'Cultured rat pituitary gonadotrophs under whole-cell voltage clamp conditions respond to the hypothalamic hormone GnRH with synchronized oscillatory changes in both cytosolic Ca2+ concentration ([Ca2+]i) and [Ca2+]i-activated, apamin-sensitive K+ current (IK(Ca)). We found, and report here for the first time, that in GnRH-stimulated cells a brief depolarizing pulse can elicit ... More
Protein and acidosis alter calcium-binding and fluorescence spectra of the calcium indicator indo-1.
AuthorsBaker AJ, Brandes R, Schreur JH, Camacho SA, Weiner MW
JournalBiophys J
PubMed ID7819496
'The fluorescent indicator indo-1 is widely used to monitor intracellular calcium concentration. However, quantitation is limited by uncertain effects of the intracellular environment on indicator properties. The goal of this study was to determine the effects of protein and acidosis on the fluorescence spectra and calcium dissociation constant (Kd) of ... More
Effect of Bay K8644 on cytosolic calcium transients and contraction in embryonic cardiac ventricular myocytes.
AuthorsLee HC, Clusin WT
JournalPflugers Arch
PubMed ID2470022
'Cytosolic calcium transients were recorded from spontaneously beating chick embryonic myocardial cell aggregates loaded with the fluorescent [Ca2+]i indicator, indo-1. Calcium transients rose rapidly from an end-diastolic [Ca2+]i of 230 +/- 18 nM to a peak systolic [Ca2+]i of 619 +/- 34 nM (n = 21). Relaxation of the transients ... More
Induction and activity of NO synthase in bone-marrow-derived macrophages are independent of Ca2+.
AuthorsHauschildt S, Lückhoff A, Mülsch A, Kohler J, Bessler W, Busse R
JournalBiochem J
PubMed ID1698056
'The aim of the present study was to analyse whether an increase in the intracellular free Ca2+ concentration ([Ca2+]i) plays a role as a signal mediating synthesis of nitric oxide (NO) in bone-marrow-derived macrophages, either by stimulating induction of NO synthase or by regulating the activity of the enzyme. Therefore ... More
Transient inositol (1,4,5) trisphosphate accumulation under vasopressin stimulation in WRK1 cells: correlation with intracellular calcium mobilization.
AuthorsMouillac B, Balestre MN, Guillon G
JournalBiochem Biophys Res Commun
PubMed ID2784680
'In the rat mammary tumoral cell line (WRK1 cells), vasopressin was previously described to stimulate a phospholipase C. In this study, we have analysed the effect of vasopressin both on intracellular calcium mobilization and on the accumulation of inositol phosphates. Maximal concentration of vasopressin simultaneously induces an accumulation of Ins(1,4,5)P3 ... More
Measurement of transient cDNA expression in mammalian cells using flow cytometric cell analysis and sorting.
AuthorsRice GC, Pennica D, Borree JA, Williams SR
JournalCytometry
PubMed ID2036916
'Generalized methods for quantitative and sensitive measurement of transient cDNA expression in mammalian cells using flow cytometry (FCM) are described. The techniques are applicable to a wide variety of cDNAs encoding intracellular or cell surface protein products through the use of immunofluorescence- or nonimmunofluorescence-based detection methods. The methods illustrated have ... More
Requirement for cGMP in nerve cell death caused by glutathione depletion.
AuthorsLi Y, Maher P, Schubert D
JournalJ Cell Biol
PubMed ID9382876
'Glutathione depletion occurs in several forms of apoptosis and is associated with Parkinson''s disease and HIV toxicity. The neurotransmitter glutamate kills immature cortical neurons and a hippocampal nerve cell line via an oxidative pathway associated with glutathione depletion. It is shown here that soluble guanylyl cyclase (sGC) activity is required ... More
The endogenous benzodiazepine receptor ligand ODN increases cytosolic calcium in cultured rat astrocytes.
AuthorsLamacz M, Tonon MC, Smih-Rouet F, Patte C, Gasque P, Fontaine M, Vaudry H
JournalBrain Res Mol Brain Res
PubMed ID8738163
'We have investigated the production of diazepam-binding inhibitor (DBI)-related peptides by astrocytes in primary culture and we have determined the effect of the octadecaneuropeptide DBI[33-50] (ODN) on the intracellular calcium concentration ([Ca2+]i) in astrocytes. Immunocytochemical labeling with antibodies against ODN showed that cultured astrocytes retain their ability to synthesize DBI ... More
HIV-1-infected T cells show a selective signaling defect after perturbation of CD3/antigen receptor.
AuthorsLinette GP, Hartzman RJ, Ledbetter JA, June CH
JournalScience
PubMed ID2899908
'The binding of antigen or monoclonal antibody to the T cell receptor for antigen or the closely associated CD3 complex causes increases in the concentration of intracellular ionized calcium and subsequent cell proliferation. By measuring second messenger production in primary cultures of human immunodeficiency virus (HIV-1)--infected T cells stimulated with ... More
Alteration of intracellular calcium flux and impairment of nuclear factor-AT translocation in T cells during acute Toxoplasma gondii infection in mice.
AuthorsHaque S, Dumon H, Haque A, Kasper LH
JournalJ Immunol
PubMed ID9862712
'Down-regulation of host immune response to Toxoplasma gondii is associated with the expression of specific cytokines, in particular IL-10, and the induction of CD4+ T cell anergy. In the present study we report that the expression of both CD4 and CD2 antigen is down-regulated during the acute phase of infection. ... More
Real-time multi-wavelength fluorescence imaging of living cells.
AuthorsMorris SJ
JournalBiotechniques
PubMed ID2331370
'We describe a new real-time fluorescence video microscope design for capturing intensified images of cells containing dual wavelength "ratio" dyes or multiple dyes. The microscope will perform real-time capture of two separate fluorescence emission images simultaneously, improving the time resolution of spatial distribution of fluorescence to video frame rates (30 ... More
Induction of apoptosis and inhibition of human gastric cancer MGC-803 cell growth by arsenic trioxide.
AuthorsZhang TC, Cao EH, Li JF, Ma W, Qin JF
JournalEur J Cancer
PubMed ID10615238
'Arsenic trioxide (As2O3), used to treat human diseases for centuries in traditional Chinese medicine, has been identified as a very effective antileukaemic agent, but its effect on solid tumours which could be more suitable for clinical treatment with arsenic compounds is still unknown. In this study, we investigated the in ... More
The BCR/ABL oncogene alters the chemotactic response to stromal-derived factor-1alpha.
AuthorsSalgia R, Quackenbush E, Lin J, Souchkova N, Sattler M, Ewaniuk DS, Klucher KM, Daley GQ, Kraeft SK, Sackstein R, Alyea EP, von Andrian UH, Chen LB, Gutierrez-Ramos JC, Pendergast AM, Griffin JD
JournalBlood
PubMed ID10590068
'The chemokine stromal-derived factor-1alpha (SDF-1alpha) is a chemoattractant for CD34(+) progenitor cells, in vitro and in vivo. The receptor for SDF-1alpha, CXCR-4, is a 7 transmembrane domain receptor, which is also a coreceptor for human immunodeficiency virus (HIV). Here we show that transformation of hematopoietic cell lines by BCR/ABL significantly ... More
Inhibition of T cell receptor expression and function in immature CD4+CD8+ cells by CD4.
AuthorsNakayama T, June CH, Munitz TI, Sheard M, McCarthy SA, Sharrow SO, Samelson LE, Singer A
JournalScience
PubMed ID2120773
'Most immature CD4+CD8+ thymocytes express only a small number of T cell receptor (TCR) molecules on their surface, and the TCR molecules they do express are only marginally capable of transducing intracellular signals. TCR expression and function was not intrinsically low in immature CD4+CD8+ thymocytes, but was found to be ... More
In vivo calcium elevations in thymocytes with T cell receptors that are specific for self ligands.
AuthorsNakayama T, Ueda Y, Yamada H, Shores EW, Singer A, June CH
JournalScience
PubMed ID1621102
'Selection of the T cell receptor (TCR) repertoire in the thymus probably involves TCR-mediated signals transduced in developing thymocytes after interaction with thymic stromal cells bearing self ligands. TCR-transduced signals should have identifiable consequences that would distinguish thymocytes whose TCRs have been engaged by self ligands from those whose TCRs ... More
Intracellular calcium changes associated with cholinergic nicotinic receptor activation in cultured myenteric plexus neurones.
AuthorsTrouslard J, Mirsky R, Jessen KR, Burnstock G, Brown DA
JournalBrain Res
PubMed ID8252380
'Intracellular free calcium concentration ([Ca2+]i) was measured in cultured explants of myenteric plexus neurones by using the fluorescent calcium indicator Indol in combination with patch-clamp techniques. The basal [Ca2+]i was 94 nM and spontaneous oscillations in the internal free calcium concentration were recorded. These oscillations were associated with bursts of ... More
Comparison of subsarcolemmal and bulk calcium concentration during spontaneous calcium release in rat ventricular myocytes.
AuthorsTrafford AW, Díaz ME, O'Neill SC, Eisner DA
JournalJ Physiol
PubMed ID8576849
'1. The aim of these experiments was to compare the time course of changes in intracellular Ca2+ concentration ([Ca2+]i) measured in the bulk cytoplasm with those estimated to occur near the sarcolemma. Sarcolemmal Na(+)-Ca2+ exchange current and [Ca2+]i were measured in single, voltage-clamped ventricular myocytes. 2. Spontaneous Ca2+ release from ... More
SH2 domain function is essential for the role of the Lck tyrosine kinase in T cell receptor signal transduction.
AuthorsStraus DB, Chan AC, Patai B, Weiss A
JournalJ Biol Chem
PubMed ID8626636
'Tyrosine kinase activity is required for signal transduction through the T cell antigen receptor (TCR). The Src family tyrosine kinase Lck appears to play a key role in the initiation of TCR signaling events. We have investigated the role of the phosphotyrosine-binding Src homology-2 (SH2), domain of Lck in TCR ... More
Time course of action of antagonists of mitochondrial Ca uptake in intact ventricular myocytes.
AuthorsZhou Z, Bers DM
JournalPflugers Arch
PubMed ID12397397
'Mitochondrial Ca uptake is important for ATP production and intracellular Ca buffering. Several agents are often used to interfere with mitochondrial Ca transport, but the use of these agents in intact cells is complicated by lack of knowledge about how rapidly these agents gain access to intracellular mitochondria. We have ... More
Agonist-induced inhibition of phosphatidylserine synthesis is secondary to the emptying of intracellular Ca2+ stores in Jurkat T-cells.
AuthorsPelassy C, Breittmayer JP, Aussel C
JournalBiochem J
PubMed ID1471993
'The biosynthesis of phosphatidylserine (PtdSer) by the serine base-exchange enzyme system, in Jurkat T-lymphocytes, was inhibited in intact cells maintained in low-Ca(2+)-containing buffer (< 10 microM-Ca2+) by using Ca2+ ionophores (A23187 or ionomycin). The rise in cytosolic Ca2+ concentration under these experimental conditions was only due to the release of ... More
Role of myoplasmic phosphate in contractile function of skeletal muscle: studies on creatine kinase-deficient mice.
AuthorsDahlstedt AJ, Katz A, Westerblad H
JournalJ Physiol
PubMed ID11389199
'1. Increased myoplasmic inorganic phosphate (P(i)) has been suggested to have an important role in skeletal muscle fatigue, especially in the early phase. In the present study we used intact fast-twitch muscle cells from mice completely deficient in creatine kinase (CK(-/-)) to test this suggestion. These CK(-/-) muscle cells provide ... More
Extracellular nucleotides mediate Ca2+ fluxes in J774 macrophages by two distinct mechanisms.
AuthorsGreenberg S, Di Virgilio F, Steinberg TH, Silverstein SC
JournalJ Biol Chem
PubMed ID3392016
'We have studied the effects of extracellular nucleotides on the cytosolic free calcium concentration [( Ca2+]i) in J774 macrophages using quin2 and indo-1 as indicator dyes. Micromolar quantities of ATP induced a biphasic increase in [Ca2+]i: a rapid and transient increase (peak I) which was due to mobilization of Ca2+ ... More
CD4 ligands inhibit the formation of multifunctional transduction complexes involved in T cell activation.
AuthorsJabado N, Pallier A, Le Deist F, Bernard F, Fischer A, Hivroz C
JournalJ Immunol
PubMed ID8977179
'Ligands binding to the CD4 molecule can inhibit TCR-mediated T cell activation. We have previously reported that transcription factors regulating the expression of the IL-2 gene, NF-AT, NF-kappaB, and AP-1, are targets of this inhibitory effect in an in vitro model using peripheral human CD4+ T cells activated by a ... More
Differences in regulation between nuclear and cytoplasmic Ca2+ in cultured smooth muscle cells.
AuthorsHimpens B, De Smedt H, Droogmans G, Casteels R
JournalAm J Physiol
PubMed ID1386189
'The free Ca2+ concentrations in the nucleus ([Ca2+]n) and cytoplasm ([Ca2+]c) of cultured smooth muscle cells were estimated using the fluorescent dye indo-1 and the ACAS 570 confocal laser microscope. In resting DDT1MF2 smooth muscle cells [Ca2+]n was found to be lower than [Ca2+]c. Both values increased transiently in response ... More
Two-photon laser-scanning microscopy: tests of objective lenses and Ca2+ probes.
AuthorsKuba K, Nakayama S
JournalNeurosci Res
PubMed ID9875569
'The characteristics of objective lenses and Ca2+-sensitive probes were examined for imaging with a two-photon laser-scanning microscope (TP-LSM). The brightness of the images of beads taken by different objectives greatly varied and depended predominantly on their numerical aperture (NA) and less on transmittance and chirping effects. Lateral and axial resolutions, ... More
Fibrinogen induces IL-8 synthesis in human neutrophils stimulated with formyl-methionyl-leucyl-phenylalanine or leukotriene B(4).
AuthorsKuhns DB, Nelson EL, Alvord WG, Gallin JI
JournalJ Immunol
PubMed ID11509634
'Human exudative neutrophils have greatly increased stores of the neutrophil chemoattractant IL-8 compared with peripheral blood cells, but the mechanism for the increase is not defined. In this report, we show that treatment of peripheral blood neutrophils with the chemotactic peptide fMLP or with leukotriene B(4) or fibrinogen results in ... More