Restricted exchange microenvironments for cell culture.
AuthorsHoh JH, Werbin JL, Heinz WF,
JournalBiotechniques
PubMed ID29570441
'Metabolite diffusion in tissues produces gradients and heterogeneous microenvironments that are not captured in standard 2D cell culture models. Here we describe restricted exchange environment chambers (REECs) in which diffusive gradients are formed and manipulated on length scales approximating those found in vivo. In REECs, cells are grown in 2D ... More