Lectin histochemical characteristics of the canine female mammary gland.
AuthorsCastagnaro M, Canese MG
JournalAm J Vet Res
PubMed ID1700652
Twelve biotinylated lectins and an avidin-biotin-peroxidase method were used to detect and localize specific carbohydrate residues on formalin-fixed, paraffin-embedded female canine mammary gland sections. Histologic sections from 3 lactating and 7 nonlactating mixed-breed dogs (age 5.6 +/- 0.35 years) were incubated with Arachis hypogea agglutinin (peanut agglutinin; PNA), Concanavalia ensiformis ... More
Complement C1q is dramatically up-regulated in brain microglia in response to transient global cerebral ischemia.
AuthorsSchäfer MK, Schwaeble WJ, Post C, Salvati P, Calabresi M, Sim RB, Petry F, Loos M, Weihe E
JournalJ Immunol
PubMed ID10799911
'Recent evidence suggests that the pathophysiology of neurodegenerative and inflammatory neurological diseases has a neuroimmunological component involving complement, an innate humoral immune defense system. The present study demonstrates the effects of experimentally induced global ischemia on the biosynthesis of C1q, the recognition subcomponent of the classical complement activation pathway, in ... More
Pepsin pretreatment allows collagen IV immunostaining of blood vessels in adult mouse brain.
AuthorsFranciosi S, De Gasperi R, Dickstein DL, English DF, Rocher AB, Janssen WG, Christoffel D, Sosa MA, Hof PR, Buxbaum JD, Elder GA
JournalJ Neurosci Methods
PubMed ID17403541
'While the brain vasculature can be imaged with many methods, immunohistochemistry has distinct advantages due to its simplicity and applicability to archival tissue. However, immunohistochemical staining of the murine brain vasculature in aldehyde fixed tissue has proven elusive and inconsistent using current protocols. Here we investigated whether antigen retrieval methods ... More
Lectin histochemistry on squamous metaplasia in different epithelial tumors of dogs.
AuthorsCastagnaro M, Canese MG
JournalVet Pathol
PubMed ID1708179
'Biotinylated lectins and avidin-biotin-peroxidase complex were used to study the correlation between cellular glycoconjugates'' expression and squamous maturation in normal canine skin and in various epithelial neoplasms. Normal skin tissue was obtained from five, male, random-source dogs, 5 to 7 years old. The tumors tested, selected from the files of ... More
MFG-E8 mediates primary phagocytosis of viable neurons during neuroinflammation.
AuthorsFricker M, Neher JJ, Zhao JW, Théry C, Tolkovsky AM, Brown GC,
JournalJ Neurosci
PubMed ID22357850
'Milk-fat globule EGF factor-8 (MFG-E8, SED1, lactadherin) is known to mediate the phagocytic removal of apoptotic cells by bridging phosphatidylserine (PS)-exposing cells and the vitronectin receptor (VR) on phagocytes. However, we show here that MFG-E8 can mediate phagocytosis of viable neurons during neuroinflammation induced by lipopolysaccharide (LPS), thereby causing neuronal ... More
Lectin histochemistry and ultrastructure of feline kidneys from six different storage diseases.
AuthorsCastagnaro M, Alroy J, Ucci AA, Glew RH
JournalVirchows Arch B Cell Pathol Incl Mol Pathol
PubMed ID2892300
'We have compared the pattern of lectin staining with the ultrastructural features of kidneys from normal cats and 19 cats with 6 different lysosomal storage diseases. The diseases studied include GM1 and GM2 gangliosidosis, mucopolysaccharidosis (MPS)-I and MPS-VI, sphingomyelin-lipidosis (i.e., Niemann-Pick disease) and mannosidosis. Ten different biotinylated lectins were used ... More
Localization of unmyelinated axons in rat skin and mucocutaneous tissue utilizing the isolectin GS-I-B4.
AuthorsPetruska JC, Streit WJ, Johnson RD
JournalSomatosens Mot Res
PubMed ID9241725
'The alpha-D-galactose specific isolectin I-B4 from Griffonia simplicifolia (GS-I-B4) labels CNS microglia and certain peripheral neurons, including a subpopulation of small dark, type B dorsal root ganglion cells, some post-ganglionic sympathetic axons, and nearly all peripheral gustatory axons. The innervation patterns of GS-I-B4 reactive sensory ganglion cells are unknown for ... More
Changes in distribution of lectin receptors in macrophages activated by Nocardia water soluble molecules.
AuthorsHoenig S, Skutelsky E, Leibovici J, Barot R
JournalCell Mol Biol (Noisy-le-grand)
PubMed ID8298432
'Lectin-histochemical studies were performed on macrophages activated by Nocardia opaca water soluble molecules (NWSM) and elicited by phosphate buffered saline (PBS) as control to demonstrate the pattern of carbohydrate residues. Ten different biotinylated lectins were used as probes and avidin-biotin-peroxidase complex (ABC) was used as visualizer. The lectins wheat germ ... More
Characterization of colonic cellular glycoconjugates in colitis and cancer-prone tamarins versus colitis and cancer-resistant primates.
AuthorsMoore R, King N, Alroy J
JournalAm J Pathol
PubMed ID3132857
'Differences in colonic secretory glycoconjugates (ie, mucin) between normal and ulcerative colitis-prone patients have been noted. Similar differences may occur in a corresponding primate model, the cotton-top tamarin (CTT), Saguinus oedipus, a New World monkey which suffers from spontaneous chronic colitis and colon cancer. Lectin reagents were used to characterize ... More
Differential and specific labeling of epithelial and vascular endothelial cells of the rat lung by Lycopersicon esculentum and Griffonia simplicifolia I lectins.
AuthorsBankston PW, Porter GA, Milici AJ, Palade GE
JournalEur J Cell Biol
PubMed ID1879433
'In the rat lung, we found that the Lycopersicon esculentum (LEA) lectin specifically binds to the epithelium of bronchioles and alveoli whereas Griffonia simplicifolia I (GS-I) binds to the endothelium of alveolar capillaries. The differential binding affinity of these lectins was examined on semithin (approximately 0.5 microns) and thin (less ... More
Effect of hypoxia on endothelial cell surface glycoprotein expression: modulation of glycoprotein IIIa and other specific surface glycoproteins.
AuthorsWeinhouse GL, Belloni PN, Farber HW
JournalExp Cell Res
PubMed ID8375475
'Exposure to hypoxia alters many aspects of endothelial cell metabolism and function; however, changes in surface glycoconjugates under these conditions have not been extensively evaluated. In the current studies, we examined surface glycoproteins of cultured bovine aortic (BAEC) and pulmonary arterial (BPAEC) endothelial cells under standard culture conditions (21% oxygen) ... More
Glycoconjugate abnormalities in cultured keratoconus stromal cells.
AuthorsYue BY, Panjwani N, Sugar J, Baum J
JournalArch Ophthalmol
PubMed ID3196213
'We examined the plasma membrane glycoconjugates of cultured corneal stromal cells derived from patients with keratoconus and healthy subjects. Using radiolabeled lectins as probes, we found that a majority of our keratoconus strains contained more binding sites for concanavalin A, Ricinus communis agglutinin I, and soybean agglutinin than did the ... More
Further studies of lectin binding by villous and extravillous trophoblast in normal and pathological pregnancy.
AuthorsThrower S, Bulmer JN, Griffin NR, Wells M
JournalInt J Gynecol Pathol
PubMed ID1917274
'Examination of human pregnancy tissues with a panel of lectins provides the opportunity to probe different aspects of carbohydrate structure. Nine biotinylated lectins [concanavalin A (con A), wheat germ agglutin (WGA), Lens culinaris A (LCH-A), Pisum sativum (PSA), Phaseolus vulgaris (PHA-E and PHA-L), Ulex europaeus 1 (UEA1), Griffonia simplicifolia (GSI ... More
A histochemical comparison of human corneal stromal glycoconjugates with eight other species. Distinct species-dictated differences in binding sites of Griffonia simplicifolia I.
AuthorsPanjwani N, Baum J
JournalHistochemistry
PubMed ID3366662
'Frozen sections of human, calf, rabbit, rat, cat, dog, goat, lamb, and hog corneas were stained with various lectins using an avidin-biotin-peroxidase complex to study glycoconjugates of stromal matrix. Staining of the stromal matrix and keratocytes with an alpha-galactose-specific lectin, Griffonia simplicifolia I (GSA-I) was species-dependent. The stromal matrices of ... More
Lectin-binding profiles in MNNG-induced shrew esophageal carcinomas.
AuthorsTakahashi H, Oyaizu T, Fujita Y, Tsubura A
JournalAnticancer Res
PubMed ID7979187
'The lectin-binding profiles of methacarn-fixed paraffin-embedded sections of N-methyl-N''-nitro-N-nitrosoguanidine (MNNG)-induced shrew esophageal lesions (papillomas, dysplasia, and carcinoma) were studied histochemically with biotinylated lectins and compared with those in adjacent normal esophageal epithelia. The lectins used for demonstrating sugar residues were soybean agglutinin (SBA), peanut agglutinin (PNA), Helix pomatia agglutinin (HPA), ... More
Matrix metalloproteinase-9 deficiency has no effect on glial scar formation after transient focal cerebral ischemia in mouse.
AuthorsCopin JC, Gasche Y
JournalBrain Res
PubMed ID17434457
Glial scar formation was investigated in wild-type and MMP-9 deficient mice during a period of 21 days after 45 min of focal cerebral ischemia by intraluminal thread occlusion of the middle cerebral artery. The results showed no differences in the kinetics of activation of microglia, oligodendrocyte precursors and reactive astrocytes ... More
Microglial cells in the central nervous system of the rabbit and rat: cytochemical identification using two different lectins.
AuthorsBoya J, Carbonell AL, Calvo JL, Borregon A
JournalActa Anat (Basel)
PubMed ID1867066
Microglial cells were selectively demonstrated in the central nervous system of adult rabbits and rats using lectin histochemistry. Biotinylated Ricinus communis agglutinin-120 (RCA-1) and biotinylated Griffonia simplicifolia B4 isolectin (GSA I-B4) were used as histochemical markers on sections of Bouin-fixed paraffin-embedded cerebrum and cerebellum. Results were quite similar using both ... More
Angiogenesis selectively requires the p110alpha isoform of PI3K to control endothelial cell migration.
AuthorsGraupera M, Guillermet-Guibert J, Foukas LC, Phng LK, Cain RJ, Salpekar A, Pearce W, Meek S, Millan J, Cutillas PR, Smith AJ, Ridley AJ, Ruhrberg C, Gerhardt H, Vanhaesebroeck B,
JournalNature
PubMed ID18449193
Phosphoinositide 3-kinases (PI3Ks) signal downstream of multiple cell-surface receptor types. Class IA PI3K isoforms couple to tyrosine kinases and consist of a p110 catalytic subunit (p110alpha, p110beta or p110delta), constitutively bound to one of five distinct p85 regulatory subunits. PI3Ks have been implicated in angiogenesis, but little is known about ... More
Microglial cells in astroglial cultures: a cautionary note.
AuthorsSaura J,
JournalJ Neuroinflammation
PubMed ID17937799
Primary rodent astroglial-enriched cultures are the most popular model to study astroglial biology in vitro. From the original methods described in the 1970's a great number of minor modifications have been incorporated into these protocols by different laboratories. These protocols result in cultures in which the astrocyte is the predominant ... More
Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice.
AuthorsPitulescu ME, Schmidt I, Benedito R, Adams RH,
JournalNat Protoc
PubMed ID20725067
The retina is a powerful experimental system for the analysis of angiogenic blood vessel growth in the postnatal organisms. The three-dimensional architecture of the vessel network and processes as diverse as endothelial cell (EC) proliferation, sprouting, perivascular cell recruitment, vessel remodeling or maturation can be investigated at high resolution. The ... More
Compartment-specific transcription factors orchestrate angiogenesis gradients in the embryonic brain.
AuthorsVasudevan A, Long JE, Crandall JE, Rubenstein JL, Bhide PG,
JournalNat Neurosci
PubMed ID18344991
Prevailing notions of cerebral vascularization imply that blood vessels sprout passively into the brain parenchyma from pial vascular plexuses to meet metabolic needs of growing neuronal populations. Endothelial cells, building blocks of blood vessels, are thought to be homogeneous in the brain with respect to their origins, gene expression patterns ... More
The effect of aging on skeletal muscle capillarization in a murine model.
AuthorsDavidson YS, Clague JE, Horan MA, Pendleton N
JournalJ Gerontol A Biol Sci Med Sci
PubMed ID10568528
Capillarization of skeletal muscle has been reported to be both maintained and reduced with advancing age. This conflict may represent methodological differences between biopsy studies. We have examined capillarization throughout two muscles, soleus and extensor digitorum longus (EDL), from a well-established colony of aging mice, and related this to fiber ... More
Lectin binding and desmin expression during necrosis, regeneration, and neurogenic atrophy of human skeletal muscle.
AuthorsHelliwell TR, Gunhan O, Edwards RH
JournalJ Pathol
PubMed ID2809885
Changes in the cytoplasm of skeletal muscle fibres during necrosis, regeneration, and neurogenic atrophy have been studied in a wide range of human neuromuscular diseases with a panel of eleven biotinylated lectins and by immunohistochemical staining for the cytoskeletal protein desmin. Increased binding of several lectins was observed in both ... More
Selective lectin-binding sites of primordial germ cells in chick and quail embryos.
AuthorsYoshinaga K, Fujimoto T, Nakamura M, Terakura H
JournalAnat Rec
PubMed ID1626721
To ascertain the histochemical characteristics of surface carbohydrates on avian primordial germ cells (PGC), we examined the distribution of binding sites for several biotinylated lectins in chick and quail embryos. Some binding sites were detected almost selectively with PGC but not with other embryonic sites. Of these, lectin from Sojanum ... More
Lectin labelling of amoeboid and ramified microglial cells in the telencephalon of ovine fetuses with the B4 isolectin from Griffonia simplicifolia.
AuthorsHewicker-Trautwein M, Schultheis G
JournalJ Comp Pathol
PubMed ID7962724
The topographical distribution of amoeboid and ramified microglial cells in the telencephalon of sheep fetuses at 60-149 days of gestation was examined by lectin histochemistry. Biotinylated B4 isolectin from Griffonia simplicifolia labelled both types of cell in coronal paraffin wax sections fixed in different fixatives. The most intense staining was ... More
Lectin histochemistry of rat placental tissues.
AuthorsBulmer JN, Peel S
JournalPlacenta
PubMed ID8899881
Lectin binding of rat trophoblast subpopulations was examined at days 10, 12 and 15 of gestation. Biotinylated lectins, specific for a range of carbohydrates, were applied to paraffin wax embedded tissues. At day 10 of pregnancy, a wide and similar range of lectins reacted with ectoplacental cone and giant cells: ... More
Corneal glycoconjugates: an ultrastructural lectin-gold study.
AuthorsLawrenson JG, Reid AR, Allt G
JournalHistochem J
PubMed ID9539207
The oligosaccharide chains of cell surface and extracellular matrix glycoconjugates are essential for the biological properties of these molecules. We have, therefore, investigated carbohydrate residues in the rat cornea using biotinylated lectin-gold probes. Fixed corneas were removed and embedded in Lowicryl HM20 or LR White. Ultrathin sections were incubated in ... More
Perineuronal nets provide a polyanionic, glia-associated form of microenvironment around certain neurons in many parts of the rat brain.
AuthorsBrückner G, Brauer K, Härtig W, Wolff JR, Rickmann MJ, Derouiche A, Delpech B, Girard N, Oertel WH, Reichenbach A
JournalGlia
PubMed ID7693589
The nature and function of previously described perineuronal nets are still obscure. In the present study their polyanionic components were demonstrated in the rat brain using colloidal iron hydroxide (CIH) staining. In subcortical regions, such as the red nucleus, cerebellar, and vestibular nuclei, most neurons were ensheathed by CIH-binding material. ... More
Lectin binding in skeletal muscle. Evaluation of alkaline phosphatase conjugated avidin staining procedures.
AuthorsKirkeby S, Bøg-Hansen TC, Moe D, Garbarsch C
JournalHistochem J
PubMed ID1717410
Cryostat sections from rat gracilis muscles were incubated with different biotinylated lectins: Con A (Concanavilin A), WGA (Wheat germ agglutinin), SBA (soybean agglutinin), GS I and GS II (Griffonia simplicifolia agglutinin), LCA (Lens culinaris agglutinin), PNA (peanut agglutinin) and PSA (Pisum sativum agglutinin). The sections were subsequently treated with alkaline ... More
Characterization of glycoconjugate expression during development of Meckel's cartilage in the rat.
AuthorsZschäbitz A, Weiser H, Stofft E, Krahn V, Gabius HJ, Khaw A, Biesalski HK
JournalAnat Embryol (Berl)
PubMed ID7717533
The staining patterns of 24 biotinylated lectins were analyzed in serial sections of the mandible of 13- to 21-day-old rat embryos by means of the avidin-biotin-peroxidase method. A ubiquitous distribution of binding sites was demonstrated after incubation with Con A (Canavalia ensiformis), DSL (Datura stramonium; except bone matrix), and WGA ... More
Differential binding of the lectins Griffonia simplicifolia I and Lycopersicon esculentum to microvascular endothelium: organ-specific localization and partial glycoprotein characterization.
AuthorsPorter GA, Palade GE, Milici AJ
JournalEur J Cell Biol
PubMed ID2328740
The lectins Griffonia simplicifolia I and Lycopersicon esculentum were used to assess the presence of endothelium-specific glycoproteins in the microvasculature of the rat myocardium, diaphragm and superficial cerebral cortex. Organs fixed by intravascular perfusion were processed to obtain semithin (0.5 micron) and thin (less than 0.1 micron) frozen sections that ... More
Lectin histochemistry of pregnant rat uterine tissues.
AuthorsPeel S, Bulmer JN
JournalJ Anat
PubMed ID8655407
Glycoconjugate residues were examined in the rat uterus at days 10, 12 and 15 of pregnancy using 17 biotinylated lectins with specificities for a variety of carbohydrate moieties. A wide variety of glycoconjugate residues were detected in the cytoplasm of some antimesometrial and mesometrial decidual cells with lectins from Canavalia ... More
Glycoconjugate expression of chondrocytes and perichondrium during hyaline cartilage development in the rat.
AuthorsZschäbitz A, Krahn V, Gabius HJ, Weiser H, Khaw A, Biesalski HK, Stofft E
JournalJ Anat
PubMed ID7591987
Alterations in the expression of glycoconjugate structures during cartilage development in the chondrocranium, nasal skeleton, Meckel's cartilage, limb buds, vertebral bodies and ribs were investigated comparatively in 13 to 21-d-old rat embryos. The binding patterns of 24 biotinylated lectins were analysed in serial sections and compared with results obtained using ... More
Alteration of cell surface carbohydrates associated with ordered and disordered proliferation of oral epithelia: a lectin histochemical study in oral leukoplakias, papillomas and carcinomas.
AuthorsVigneswaran N, Peters KP, Hornstein OP, Diepgen TL
JournalCell Tissue Kinet
PubMed ID2302731
Cell surface carbohydrates in healthy oral mucosa (n = 15), leukoplakias without (n = 48) and with (n = 62) dysplasia, oral papillomas (n = 6) and squamous cell carcinomas (SCCs) (n = 40) were examined using the lectins peanut agglutinin (PNA), Ulex europaeus agglutinin I (UEA I), soybean agglutinin ... More
Distribution of the major xenoantigen (gal (alpha 1-3)gal) for pig to human xenografts.
AuthorsMcKenzie IF, Xing PX, Vaughan HA, Prenzoska J, Dabkowski PL, Sandrin MS
JournalTranspl Immunol
PubMed ID7953322
We have previously demonstrated that the major epitope in pig tissues detected by naturally occurring human IgM antibodies is galactose (alpha 1-3)galactose. Subsequent biochemical studies demonstrated this epitope to be present on molecules (Mr40-220kDa) on both endothelial cells and lymphocytes. The objective of the present study was to define the ... More
Affinity-purified antibodies against alpha-galactosyl residues from human serum: comparison of their binding in bovine testicular tissue with that of the Griffonia simplicifolia lectin (GSI-B4) and impact of labeling on epitope localization.
AuthorsDong X, Amselgruber WM, Kaltner H, Gabius HJ, Sinowatz F
JournalEur J Cell Biol
PubMed ID8549596
alpha-Galactosyl residues in the carbohydrate part of cellular glycoconjugates can serve as cell type-associated markers and are implicated in intercellular adhesion and biosignaling. This biological significance explains the interest to characterize probes with respective specificity as the Griffonia simplicifolia I-isolectin B4. Due to the documented occurrence of an alpha-galactoside-binding immunoglobulin ... More
Temporal characterization of microglia, IL-1 beta-like immunoreactivity and astrocytes in the dentate gyrus of hippocampal organotypic slice cultures.
AuthorsColtman BW, Ide CF
JournalInt J Dev Neurosci
PubMed ID8960978
These studies demonstrate that murine hippocampal slice cultures possess neural-immune elements that show responses parallel to comparable in vivo models of neural-immune activation. Using immunocytochemical techniques, this study characterized the phenotypes of specific glial elements and the expression of the cytokine, interleukin-1 (IL-1 beta), in the hippocampal dentate gyrus over ... More
Mechanically Activated Piezo Channels Mediate Touch and Suppress Acute Mechanical Pain Response in Mice.
Authors
JournalCell Rep
PubMed ID30726728
EphrinB2 controls vessel pruning through STAT1-JNK3 signalling.
Authors
JournalNat Commun
PubMed ID25807892
TRPV1 channels are critical brain inflammation detectors and neuropathic pain biomarkers in mice.
Authors
JournalNat Commun
PubMed ID28489079
Synthetic microparticles conjugated with VEGF165 improve the survival of endothelial progenitor cells via microRNA-17 inhibition.
Authors
JournalNat Commun
PubMed ID28963481
Suppression of Choroidal Neovascularization by AAV-Based Dual-Acting Antiangiogenic Gene Therapy.
Authors
JournalMol Ther Nucleic Acids
PubMed ID30825671
Neuronal pentraxin 2 is required for facilitating excitatory synaptic inputs onto spinal neurons involved in pruriceptive transmission in a model of chronic itch.
Authors
JournalNat Commun
PubMed ID35501343
Identification of a population of peripheral sensory neurons that regulates blood pressure.
Authors
JournalCell Rep
PubMed ID34077727
Glioma-Induced Alterations in Neuronal Activity and Neurovascular Coupling during Disease Progression.
Authors
JournalCell Rep
PubMed ID32294436
Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons.
Authors
JournalNat Commun
PubMed ID29691410
Acetylated tubulin is essential for touch sensation in mice.