New ratiometric fluorescent calcium indicators with moderately attenuated binding affinities.
AuthorsGee KR, Archer EA, Lapham LA, Leonard ME, Zhou ZL, Bingham J, Diwu Z
JournalBioorg Med Chem Lett
PubMed ID10915039
Mono-halogenated derivatives of the calcium indicators fura-2 and indo-1 were synthesized and their spectroscopic properties evaluated. Halogenation ortho or para to the bridging oxygen in the BAPTA nucleus had a more pronounced weakening effect on binding affinity than in the meta position in the fura derivatives. Two new excitation ratioable ... More