ImaGene Green™ C12FDG lacZ 基因表达试剂盒
ImaGene Green&trade; C<sub>12</sub>FDG <i>lacZ</i> 基因表达试剂盒
引用和文献 (32)
Invitrogen™

ImaGene Green™ C12FDG lacZ 基因表达试剂盒

ImaGene Green™ lacZ 基因表达试剂盒包含一种基于荧光素的半乳糖苷酶底物，此底物经共价修饰，以包含12-碳亲脂性部分 C12FDG。一旦进入细胞，底物即被 β-半乳糖苷酶裂解，产生荧光产物，很可能通过与细胞膜上的亲脂性尾端结合，从而完好地保留在细胞内。除 C12FDG 之外，该试剂盒还包括了广谱了解更多信息
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货号数量
I29041 kit
货号 I2904
价格（CNY)
7,913.00
飞享价
Ends: 31-Dec-2025
10,726.00
共减 2,813.00 (26%)
Each
添加至购物车
数量:
1 kit
价格（CNY)
7,913.00
飞享价
Ends: 31-Dec-2025
10,726.00
共减 2,813.00 (26%)
Each
添加至购物车
ImaGene Green™ lacZ 基因表达试剂盒包含一种基于荧光素的半乳糖苷酶底物，此底物经共价修饰，以包含12-碳亲脂性部分 C12FDG。一旦进入细胞，底物即被 β-半乳糖苷酶裂解，产生荧光产物，很可能通过与细胞膜上的亲脂性尾端结合，从而完好地保留在细胞内。除 C12FDG 之外，该试剂盒还包括了广谱 β-半乳糖苷酶抑制剂 PETG 和二磷酸氯喹，可抑制底物的酸性水解。
仅供科研使用。不可用于诊断程序。
规格
检测β-Gal (lacZ) 检测
检测方法荧光
产品线ImaGene Green
产品类型β-半乳糖苷酶报告基因测定系统
数量1 kit
运输条件室温
底物C12FDG
底物属性化学底物
底物类型β-Gal 底物
目标酶β-半乳糖苷酶
产品规格试剂盒
Unit SizeEach
内容与储存
储存在冰箱（-5 至 -30°C）中并避光。

引用和文献 (32)

引用和文献
Abstract
Rapid flow cytometric method for measuring senescence associated beta-galactosidase activity in human fibroblasts.
Authors:Noppe G, Dekker P, de Koning-Treurniet C, Blom J, van Heemst D, Dirks RW, Tanke HJ, Westendorp RG, Maier AB,
Journal:Cytometry A
PubMed ID:19777541
'Senescence associated-beta-galactosidase (SA-beta-gal) activity is a widely used marker for cellular senenescence. SA-beta-gal activity is routinely detected cytochemically, manually discriminating negative from positive cells. This method is time-consuming, subjective and therefore prone to operator-error. We aimed to optimize a flow cytometric method described by other workers using endothelial cells to ... More
Optical imaging fiber-based single live cell arrays: a high-density cell assay platform.
Authors:Biran I, Walt DR
Journal:Anal Chem
PubMed ID:12141663
'A high-density, ordered array containing thousands of microwells is fabricated on an optical imaging fiber. Each individually addressable microwell is used to accommodate a single living cell. A charged coupled device (CCD) detector is employed to monitor and spatially resolve the fluorescence signals obtained from each individual cell, allowing simultaneous ... More
An optimized electroporation protocol applicable to a wide range of cell lines.
Authors:Baum C, Forster P, Hegewisch-Becker S, Harbers K
Journal:Biotechniques
PubMed ID:7873174
'A number of transfection methods for mammalian cells are available; however, many cell lines may appear resistant to efficient transfection, or at best, necessitate lengthy optimization procedures in recommended protocols. We describe here an electroporation protocol that yields highly efficient gene transfer (20%-100% of surviving cells) in all 19 cell ... More
Mapping mechanisms and charting the time course of premature cell senescence and apoptosis: lysosomal dysfunction and ganglioside accumulation in endothelial cells.
Authors:Patschan S, Chen J, Gealekman O, Krupincza K, Wang M, Shu L, Shayman JA, Goligorsky MS,
Journal:Am J Physiol Renal Physiol
PubMed ID:17928415
'Endothelial cells subjected to glycated collagen I develop premature senescence within 3-5 days, as revealed by increased senescence-associated beta-galactosidase activity, decreased proliferation, and an increase in cell size. Here, we analyzed the time course and possible mechanisms of this process. Lysosomal integrity studies revealed a rapid collapse of pH gradient ... More
Use of fluorescence-activated cell sorting for rapid isolation of insect cells harboring recombinant baculovirus.
Authors:Sommerfelt MA, Sorscher EJ
Journal:Methods Cell Biol
PubMed ID:7877509
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