Endoplasmic Reticulum Stress and Apoptosis Contribute to the Pathogenesis of Dominantly Inherited Isolated GH Deficiency Due to GH1 Gene Splice Site Mutations.
AuthorsAriyasu D, Yoshida H, Yamada M, Hasegawa Y,
Journal
PubMed ID23736291
Dominantly inherited isolated GH deficiency is mainly caused by a heterozygous donor site mutation of intron 3 in the GH1 gene. An exon 3 deletion in GH (del32-71 GH) is produced from a mutant allele, whereas wild-type GH is produced from the other allele. Several studies have demonstrated a dominant ... More
Characterization of Differentiated SH-SY5Y as Neuronal Screening Model Reveals Increased Oxidative Vulnerability.
Authors
JournalJ Biomol Screen
PubMed ID26738520
JUND regulates pancreatic ß cell survival during metabolic stress.
AuthorsGood AL, Cannon CE, Haemmerle MW, Yang J, Stanescu DE, Doliba NM, Birnbaum MJ, Stoffers DA
JournalMol Metab
PubMed ID31023625
'In type 2 diabetes (T2D), oxidative stress contributes to the dysfunction and loss of pancreatic ß cells. A highly conserved feature of the cellular response to stress is the regulation of mRNA translation; however, the genes regulated at the level of translation are often overlooked due to the convenience of ... More
CD95-mediated apoptosis in Burkitt's lymphoma B-cells is associated with Pim-1 down-regulation.
AuthorsMatou-Nasri S, Rabhan Z, Al-Baijan H, Al-Eidi H, Yahya WB, Al Abdulrahman A, Almobadel N, Alsubeai M, Al Ghamdi S, Alaskar A, AlBalwi M, Alzahrani M, Alabdulkareem I
JournalBiochim Biophys Acta Mol Basis Dis
PubMed ID27641442
'B-cells of the high-grade non-Hodgkin lymphoma Burkitt''s lymphoma (BL) overexpress survival oncoproteins, including the proviral integration site for Moloney murine leukaemia virus kinase (Pim)-1, and become apoptosis resistant. Activated death receptor CD95 after ligation with anti-CD95 monoclonal antibody (mAb) resulted in the regression of BL via induction of apoptosis, suggesting ... More
Impaired serotonin communication during juvenile development in rats diminishes adult sperm quality.
AuthorsDíaz-Ramos J, Flores-Flores M, Ayala ME, Aragón-Martínez A
JournalSyst Biol Reprod Med
PubMed ID29788785
'Spermatogenesis and steroidogenesis are testicular functions regulated by gonadotrophins as well as other factors, including serotonin. Testicular serotonin acts as an autocrine regulator of testosterone secretion, but studies on its role in spermatogenesis and sperm quality are scarce. Here, we analyzed the effects of intratesticular inhibition of serotonin synthesis on ... More
Evaluations of Acute and Sub-Acute Biological Effects of Narrowband and Moderate-Band High Power Electromagnetic Waves on Cellular Spheroids.
AuthorsGibot L, Kolosnjaj-Tabi J, Bellard E, Chretiennot T, Saurin Q, Catrain A, Golzio M, Vézinet R, Rols MP
JournalSci Rep
PubMed ID31653929
'High power electromagnetic signals can disrupt the functioning of electronic devices. As electromagnetism plays a role in cells homeostasis, such electromagnetic signals could potentially also alter some physiological processes. Herein we report on distinct biological parameters assessment after cellular spheroids exposure to high power electromagnetic signals, such as the ones ... More
Tristetraprolin inhibits mitochondrial function through suppression of α-Synuclein expression in cancer cells.
Authors
JournalOncotarget
PubMed ID28410208
SOX2 is required for adult human muller stem cell survival and maintenance of progenicity in vitro.
Authors
JournalInvest Ophthalmol Vis Sci
PubMed ID20739473
Non-Thermal Plasma Jet-Treated Medium Induces Selective Cytotoxicity against Mycobacterium tuberculosis-Infected Macrophages.
Authors
JournalBiomedicines
PubMed ID35740265
Morphokinetic-related response to stress in individually cultured bovine embryos.
AuthorsSilva T, Santos EC, Annes K, Soares CA, Leite RF, Lima CB, Milazzotto MP
JournalTheriogenology
PubMed ID27298151
The kinetics of in vitro-produced (IVP) bovine embryos is related to embryo viability, metabolism, and epigenetic patterns. Therefore, we believe that embryos with different speeds of development also respond differently to stress. In the present study, we performed global metabolic analysis (matrix-assisted laser desorption ionization time of flight mass spectrometry [MALDI-TOF]) ... More
Targeted intracellular protein delivery based on hyaluronic acid-green tea catechin nanogels.
AuthorsLiang K, Ng S, Lee F, Lim J, Chung JE, Lee SS, Kurisawa M
JournalActa Biomater
PubMed ID26785145
A novel ternary nanogel based on the self-assembly of hyaluronic acid-epigallocatechin gallate conjugates (HA-EGCG), linear polyethylenimine (PEI) and Granzyme B (GzmB) in an aqueous environment was developed for the targeted intracellular delivery of GzmB into cancer cells. Lysozyme-encapsulated HA-EGCG nanogels were first prepared and characterized. HA-EGCG nanogels exhibited smaller particle ... More
Precision Optogenetic Tool for Selective Single- and Multiple-Cell Ablation in a Live Animal Model System.
AuthorsMakhijani K, To TL, Ruiz-González R, Lafaye C, Royant A, Shu X
JournalCell Chem Biol
PubMed ID28065655
Cell ablation is a strategy to study cell lineage and function during development. Optogenetic methods are an important cell-ablation approach, and we have previously developed a mini singlet oxygen generator (miniSOG) tool that works in the living Caenorhabditis elegans. Here, we use directed evolution to generate miniSOG2, an improved tool for cell ... More
A PDX1-ATF transcriptional complex governs ß cell survival during stress.
AuthorsJuliana CA, Yang J, Cannon CE, Good AL, Haemmerle MW, Stoffers DA
JournalMol Metab
PubMed ID30174228
Loss of insulin secretion due to failure or death of the insulin secreting ß cells is the central cause of diabetes. The cellular response to stress (endoplasmic reticulum (ER), oxidative, inflammatory) is essential to sustain normal ß cell function and survival. Pancreatic and duodenal homeobox 1 (PDX1), Activating transcription factor ... More
Metabolic stress activates an ERK/hnRNPK/DDX3X pathway in pancreatic ß cells.
AuthorsGood AL, Haemmerle MW, Oguh AU, Doliba NM, Stoffers DA
JournalMol Metab
PubMed ID31178390
Pancreatic ß cell failure plays a central role in the development of type 2 diabetes (T2D). While the transcription factors shaping the ß cell gene expression program have received much attention, the post-transcriptional controls that are activated in ß cells during stress are largely unknown. We recently identified JUND as ... More
Targeting CD52 does not affect murine neuron and microglia function.
AuthorsEllwardt E, Vogelaar CF, Maldet C, Schmaul S, Bittner S, Luchtman D
JournalEur J Pharmacol
PubMed ID31962100
The humanized anti-CD52 antibody alemtuzumab is successfully used in the treatment of multiple sclerosis (MS) and is thought to exert most of its therapeutic action by depletion and repopulation of mainly B and T lymphocytes. Although neuroprotective effects of alemtuzumab have been suggested, direct effects of anti-CD52 treatment on glial ... More