Transient and long-lasting openings of the mitochondrial permeability transition pore can be monitored directly in intact cells by changes in mitochondrial calcein fluorescence.
AuthorsPetronilli V, Miotto G, Canton M, Brini M, Colonna R, Bernardi P, Di Lisa F
JournalBiophys J
PubMed ID9929477
'The occurrence and the mode of opening of the mitochondrial permeability transition pore (MTP) were investigated directly in intact cells by monitoring the fluorescence of mitochondrial entrapped calcein. When MH1C1 cells and hepatocytes were loaded with calcein AM, calcein was also present within mitochondria, because (i) its mitochondrial signal was ... More
Effect of transient and permanent permeability transition pore opening on NAD(P)H localization in intact cells.
'To study the effect of mitochondrial permeability transition pore (PTP) opening on NAD(P)H localization, intact cells were exposed to the Ca(2+) ionophore A23187. PTP opening, mitochondrial membrane potential, mitochondrial volume, and NAD(P)H localization were assessed by time-lapse laser confocal microscopy using the calcein-cobalt technique, tetramethylrhodamine methyl ester, MitoTracker, and NAD(P)H ... More
Measuring apoptosis at the single cell level.
AuthorsBouchier-Hayes L, Muñoz-Pinedo C, Connell S, Green DR,
JournalMethods
PubMed ID18314052
The use of live cell microscopy has made a number of contributions to the study of apoptosis. Many of the tools and techniques are available that allow us to image the key events that occur during cell death including mitochondrial outer membrane permeabilization, mitochondrial transmembrane potential changes, translocation of Bcl-2 ... More
The mechanisms of apoptosis in biology and medicine: a new focus for ophthalmology.
AuthorsTempestini A, Schiavone N, Papucci L, Witort E, Lapucci A, Cutrì M, Donnini M, Capaccioli S
JournalEur J Ophthalmol
PubMed ID12749672
Defects in apoptosis (programmed cell death) have recently emerged as being closely involved in the pathogenesis of most ocular diseases and, therefore, apoptosis is now a topic of exponential interest in ophthalmology. This review summarizes recent works on mechanisms of apoptosis, from its initiation and modulation to the switching-on of ... More
Therapeutic Potential of a Novel Necrosis Inhibitor, 7-Amino-Indole, in Myocardial Ischemia-Reperfusion Injury.
Authors
JournalHypertension
PubMed ID29661840
Quantitative proteomic analysis of A549 cells infected with human respiratory syncytial virus.
Authors
JournalMol Cell Proteomics
PubMed ID20647383
Mitochondrial Dysfunction and Alterations in Mitochondrial Permeability Transition Pore (mPTP) Contribute to Apoptosis Resistance in Idiopathic Pulmonary Fibrosis Fibroblasts.
Authors
JournalInt J Mol Sci
PubMed ID34360637
Mitochondrial DNA drives noncanonical inflammation activation via cGAS-STING signaling pathway in retinal microvascular endothelial cells.
Authors
JournalCell Commun Signal
PubMed ID33115500
Fundc1-dependent mitophagy is obligatory to ischemic preconditioning-conferred renoprotection in ischemic AKI via suppression of Drp1-mediated mitochondrial fission.
Authors
JournalRedox Biol
PubMed ID31901590
Increased fatty acid metabolism attenuates cardiac resistance to β-adrenoceptor activation via mitochondrial reactive oxygen species: A potential mechanism of hypoglycemia-induced myocardial injury in diabetes.
Authors
JournalRedox Biol
PubMed ID35462320
Dynasore protects ocular surface mucosal epithelia subjected to oxidative stress by maintaining UPR and calcium homeostasis.
Authors
JournalFree Radic Biol Med
PubMed ID32791188
Association of hypoxia and mitochondrial damage associated molecular patterns in the pathogenesis of vein graft failure: a pilot study.
Authors
JournalTransl Res
PubMed ID32861831
Amplification of Mitochondrial Activity in the Healing Response Following Rotator Cuff Tendon Injury.
Authors
JournalSci Rep
PubMed ID30451947
Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output.