Differentiation of mouse embryonic stem cells following RNAi-mediated silencing of OCT4 and Nanog.
AuthorsHough SR, Clements I, Welch PJ, Wiederholt KA,
JournalStem Cells
PubMed ID16456133
RNAi holds great promise as a tool to study the basic biology of stem cells or to direct differentiation in a specific manner. Barriers to achieving efficient and specific gene silencing in RNAi experiments include limitations in transfection efficiency and in the efficacy and specificity of RNAi silencing effectors. Here, ... More