Yeast mitochondrial protein, Nfs1p, coordinately regulates iron-sulfur cluster proteins, cellular iron uptake, and iron distribution.
AuthorsLi J, Kogan M, Knight SA, Pain D, Dancis A
JournalJ Biol Chem
PubMed ID10551871
'Nfs1p is the yeast homolog of the bacterial proteins NifS and IscS, enzymes that release sulfur from cysteine for iron-sulfur cluster assembly. Here we show that the yeast mitochondrial protein Nfs1p regulates cellular and mitochondrial iron homeostasis. A strain of Saccharomyces cerevisiae, MA14, with a missense NFS1 allele (I191S) was ... More
High-resolution analyses of two different classes of tumor cells in situ tagged with alternative histochemical marker genes.
AuthorsLin WC, Pretlow TP, Pretlow TG, Culp LA
JournalAm J Pathol
PubMed ID1466397
'To evaluate interactions of two different tumor cell classes during the establishment of micrometastases at the single-cell level, two different BALB/c 3T3 tumor cell derivatives were established that harbor different histochemical marker genes: bacterial lacZ in a EJ-Harvey ras transformant (abbreviated LZEJ cells) and human placental alkaline phosphatase (ALP) gene ... More
RNA-RNA in situ hybridization using digoxigenin-labeled probes: the use of high-molecular-weight polyvinyl alcohol in the alkaline phosphatase indoxyl-nitroblue tetrazolium reaction.
AuthorsDe Block M, Debrouwer D
JournalAnal Biochem
PubMed ID7507651
'A nonradioactive RNA-RNA in situ hybridization method using digoxigenin-labeled probes is described. The visualization of hybrids is done using the indoxyl-nitro blue tetrazolium alkaline phosphatase reaction. The addition of polyvinyl alcohols of high molecular weight (40-100 kDa) to the reaction medium enhances the alkaline phosphatase reaction and prevents diffusion of ... More
Quantitative succinic dehydrogenases histochemistry. A comparison of different tetrazolium salts.
AuthorsLippold HJ
JournalHistochemistry
PubMed ID6186649
Physiological assessment of bacteria using fluorochromes.
AuthorsMcFeters GA, Yu FP, Pyle BH, Stewart PS
JournalJ Microbiol Methods
PubMed ID11538412
This minireview focuses on the application of fluorogenic compounds in the detection of bacteria with particular emphasis on the assessment of physiological activity using epifluorescence microscopy. Microbiological applications of several related methods will also be reviewed. ... More
Tetrazolium salts and formazans.
AuthorsAltman FP
JournalProg Histochem Cytochem
PubMed ID792958
The history of the tetrazolium salts and formazans goes back 100 years, to when Friese (1875) reacted benzene diazonium nitrate with nitromethane, to produce a cherry-red "Neue Verbindung". This was the first formazan. 19 years later, Von Pechmann and Runge (1894) oxidised a formazan to produce the first tetrazolium salt. ... More
Comparison of the fluorescent redox dye 5-cyano-2,3-ditolyltetrazolium chloride with p-iodonitrotetrazolium violet to detect metabolic activity in heat-stressed Listeria monocytogenes cells.
AuthorsBovill RA, Shallcross JA, Mackey BM
JournalJ Appl Bacteriol
PubMed ID7989262
The fluorogenic redox indicator 5-cyano-2,3-ditolyltetrazolium chloride (CTC) was compared with the chromogenic p-iodonitrotetrazolium violet (INT) and conventional methods to assess cellular viability. Mild heat treatment was used as well-controlled method for producing non-viable and sub-lethally injured cells. CTC gave an underestimation of the viability of Listeria monocytogenes cells when compared ... More
The bHLH transcription factor Olig2 promotes oligodendrocyte differentiation in collaboration with Nkx2.2.
AuthorsZhou Q, Choi G, Anderson DJ
JournalNeuron
PubMed ID11567617
Olig2, a basic helix-loop-helix (bHLH) transcription factor, is expressed in a restricted domain of the spinal cord ventricular zone that sequentially generates motoneurons and oligodendrocytes. Just prior to oligo-dendrocyte precursor formation, the domains of Olig2 and Nkx2.2 expression switch from being mutually exclusive to overlapping, and Neurogenins1 and 2 are ... More
Destructive proteolysis by cysteine proteases in antigen presentation of ovalbumin.
AuthorsRodriguez GM, Diment S
JournalEur J Immunol
PubMed ID7621859
Most native antigens require digestion by acidic proteases in order to be recognized in the context of major histocompatibility complex class II by T helper cells (Th). We have studied the roles of three different acidic proteases, cathepsin D, cathepsin B and cathepsin L, in the processing of ovalbumin (OVA) ... More
Photoconversion of diaminobenzidine with different fluorescent neuronal markers into a light and electron microscopic dense reaction product.
AuthorsLübke J
JournalMicrosc Res Tech
PubMed ID7679591
This article describes methods for photoconverting diaminobenzidine (DAB) into a stable, light and electron microscopically visible dark reaction product in neurons which contain a fluorescent dye. Photoconversion of DAB has been achieved so far with the following fluorescent dyes: rhodamine labeled latex microspheres (RLM), 4,6-diamidino-2-phenylindole (DAPI), 5,7-dihydroxytryptamine (5,7-DHT), Fast Blue ... More