Transcriptome-wide identification of RNA binding sites by CLIP-seq.
AuthorsMurigneux V, Saulière J, Roest Crollius H, Le Hir H,
JournalMethods
PubMed ID23545196
An emergent strategy for the transcriptome-wide study of protein-RNA interactions is CLIP-seq (crosslinking and immunoprecipitation followed by high-throughput sequencing). We combined CLIP-seq and mRNA-seq to identify direct RNA binding sites of eIF4AIII in human cells. This RNA helicase is a core constituant of the Exon Junction Complex (EJC), a multifunctional ... More