Agarose LE, Molecular Biology Grade, Ultrapure - FAQs

What are the applications for Agarose LE, Molecular Biology Grade, Ultrapure?

Applications:
1. Routine separation of DNA or RNA fragments.
2. PCR analysis.
3. Blotting applications such as southern blots and northern blots.
4. Protein analysis such as immunodiffusion, rocket assays and immuno electrophoresis.

What are the separation guidelines for Agarose LE, Molecular Biology Grade, Ultrapure?

Range of separation in 1X TAE: 0.6%: 20,000-1000 bp
0.8%: 12,000-500 bp
1.0%: 8000-300 bp
1.2%: 6000-200 bp
1.5%: 3500-100 bp
2.0%: 2000-50 bp

Range of separation in 1X TBE:
0.6%: 15,000-1000 bp
0.8%: 10,000-500 bp
1.0%: 7000-250 bp
1.2%: 5000-200 bp
1.5%: 3000-100 bp
2.0%: 2000-50 bp

How do I prepare an agarose gel?

We recommend the following to prepare an agarose gel:

1. Weigh out the desired amount of agarose and place it in an Erlenmeyer flask with a measured amount of electrophoresis buffer. Make sure to use the same electrophoresis buffer in the gel as for the running buffer.
Example: For a 0.8% gel, add 0.8 g of agarose and 100 mL of TBE Buffer (1X), to a 200 mL flask. The larger flask insures against the agarose boiling over.
2. Dissolve the agarose in a boiling water bath or in a revolving-plate microwave oven. All the grains of agarose should be dissolved, and the solution should be clear.
3. Cool the solution to 60°C (70°C for concentrations 2% or above) and pour immediately.
4. Allow the gel to set for 30 minutes before using.