To make a 20 mM solution of Adenosine-5'-triphosphate disodium salt hydrate, 98% (Cat. No. J61125.14) for assays:
1. Calculate the amount of Adenosine-5'-triphosphate disodium salt hydrate, 98% needed:
- Molecular Weight: 551.15 g/mol
- Desired concentration: 20 mM
- Assuming that the volume of the solution is 1 L, the concentration is 0.02 mol/L
2. Amount needed (in grams) = Concentration (mol/L) x Molecular Weight (g/mol) x Volume (L)
- Amount needed = 0.02 mol/L x 551.15 g/mol x 1 L = 11.023 g
3. Dissolve the calculated amount in water:
- Dissolve 11.023 g of Adenosine-5'-triphosphate disodium salt hydrate, 98% in 1 L of water.
4. Adjust the volume if necessary:
- If you need a different volume, adjust the amount of Adenosine-5'-triphosphate disodium salt hydrate, 98% proportionally.
Note: Ensure the solution is clear and colorless to pale yellow, as per the solubility information provided.