LDS-sample buffer (4X), non-reducing - FAQs

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2 个常见问题解答

At what temperature should I store LDS-sample buffer (4X), non-reducing (Cat. No. J61894.xx)?

LDS-sample buffer (4X), non-reducing should be stored at room temperature (20-25 degrees Celsius) upon receipt.

What protocol should I use for LDS-sample buffer (4X), non-reducing (Cat. No. J61894.xx)?

We recommend the following protocol for use of LDS-sample buffer (4X), non-reducing:
1. Mix one part LDS sample buffer to three parts protein (e.g., 5 µL sample buffer + 15 µL protein sample). Failure to properly dilute may result in a diffuse dye front.
2. Heat mixture at 100°C for 3-5 minutes.
3. Allow sample to cool back to room temperature.
4. Load sample onto SDS-polyacrylamide gel and begin electrophoresis.
5. Stop electrophoresis when the dye front nears the bottom of the gel.