MOPS-SDS running buffer (20X)
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MOPS-SDS running buffer (20X)
Thermo Scientific Chemicals

MOPS-SDS running buffer (20X)

MOPS-SDS running buffer (20X) is a buffer frequently used for separating medium- to large-sized proteins on a gel.
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数量:
500 mL
1 L
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货号 J62847.K2
又称 J62847-K2
价格（CNY)
-
数量:
1 L
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规格
化学名称或材料MOPS-SDS running buffer
健康危害 1H315-H319-H335
健康危害 2GHS H Statement
H315-H319-H335
Causes skin irritation.
Causes serious eye irritation.
May cause respiratory irritation.
健康危害 3P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P332+P313-P362-P501c
建议的储存条件Ambient temperatures
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MOPS SDS Running Buffer is recommended for separating medium- to large-sized proteins. MOPS-SDS Running Buffer is suitable for protein gel electrophoresis. Use of MOPS buffer allows proteins to run slower than when using MES buffer. This buffer is used in 1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis - See more at:

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

  • MOPS-SDS running buffer is a zwitterionic, morpholinic buffer that allows the separation of medium- to large-sized proteins

Application

  • MOPS is used as a running buffer in electrophoresis. The use of MOPS buffer allows proteins to run slower than when MES buffer is used
  • MOPS-SDS running buffer, 20X concentrated, is formulated to be used for running proteins on an SDS gel
  • This buffer contains SDS detergent to maintain the denaturation of proteins and MOPS to buffer the pH
  • It is commonly used for cell culture media and protein purification in chromatography
  • MOPS is frequently applied in buffered culture media for bacteria, yeast, and mammalian cells
  • It is also applied in the bicinchoninic acid (BCA) assay
  • It is used in separating RNA in agarose gels
RUO – Research Use Only
MM Santoro.; DW Bolen. A test of the linear extrapolation of unfolding free energy changes over an extended denaturant concentration range. Biochemistry. 1992, 31, (20), 4901-4907.