Ethidium bromide de-staining bags, with activation soln. - FAQs

What is the maximum capacity of ethidium bromide for each bag for the Ethidium bromide de-staining bags, with activation soln.?

Each ethidium bromide de-staining bag has a maximum capacity of 5 mg of ethidium bromide.

Can I reuse the Ethidium bromide de-staining bags, with activation soln.?

We recommend a one-time use for the de-staining bag for ease and convenience. Reusing the de-staining bag would require you to keep an accurate decontamination log to avoid saturating the resin.

What are the instructions for using the Ethidium bromide de-staining bags, with activation soln.?

We recommend following instructions for using the Ethidium bromide de-staining bags, with activation soln.:

1. Wet the de-staining bag by placing it into approximately 5 mL of activation solution or water for 30 minutes.
2. Add the wet bag to the solution containing ethidium bromide or stained gel.
3. Allow the bag to mix overnight with gentle agitation or rocking. The gentle agitation increases the speed of adsorption.
4. Remove the bag and dispose of as hazardous waste according to your institution's recommended procedures. Properly dispose of the gel or buffer solution.