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Thermo Scientific Chemicals
Agarose, low gelling temperature, Molecular Biology Reagent
CAS: 9012-36-6
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化学标识符
CAS9012-36-6
IUPAC Name(2S,3R,4S,5R,6R)-2-{[(1S,3S,4S,5S,8R)-3-{[(2S,3R,4S,5S,6R)-2-{[(1S,3R,4S,5S,8R)-3,4-dihydroxy-2,6-dioxabicyclo[3.2.1]octan-8-yl]oxy}-3,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy}-4-hydroxy-2,6-dioxabicyclo[3.2.1]octan-8-yl]oxy}-6-(hydroxymethyl)oxane-3,4,5-triol
Molecular FormulaC24H38O19
InChI KeyMJQHZNBUODTQTK-WKGBVCLCSA-N
SMILESOC[C@H]1O[C@@H](O[C@@H]2[C@@H]3CO[C@H]2[C@H](O)[C@H](O[C@H]2[C@@H](O)[C@@H](CO)O[C@@H](O[C@@H]4[C@@H]5CO[C@H]4[C@H](O)[C@H](O)O5)[C@@H]2O)O3)[C@H](O)[C@@H](O)[C@H]1O
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规格
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规格表Appearance (Color)White to pale cream
FormPowder
Assay (special)Electroendosmosis (EEO): ≤ 0.1%
Assay (special)Gel strength (1.0%): ≥ 200 gm/cm2
Assay (special)Gelling temperature: 26 to 30°C
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Agarose is useful in separation of nucleic acids electrophoretically because agarose gels have larger pore sizes than cross linked acrylamide gels at low concentrations and in chromatographic separations by either beading the agarose or cross-linking it. Agarose gels are also used in immunoelectrophoresis (IEP) and double-diffusion Ouchterlony plates to demonstrate antibody-antigen reactions.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Agarose is useful in separation of nucleic acids electrophoretically because agarose gels have larger pore sizes than cross linked acrylamide gels at low concentrations and in chromatographic separations by either beading the agarose or cross-linking it. Agarose gels are also used in immunoelectrophoresis (IEP) and double-diffusion Ouchterlony plates to demonstrate antibody-antigen reactions.
Solubility
Soluble in water.
Notes
Gel strength (1.5% w/v):>500g/cm^2; Gelling temperature: <24-28°C; EEO: <0.12
Agarose is useful in separation of nucleic acids electrophoretically because agarose gels have larger pore sizes than cross linked acrylamide gels at low concentrations and in chromatographic separations by either beading the agarose or cross-linking it. Agarose gels are also used in immunoelectrophoresis (IEP) and double-diffusion Ouchterlony plates to demonstrate antibody-antigen reactions.
Solubility
Soluble in water.
Notes
Gel strength (1.5% w/v):>500g/cm^2; Gelling temperature: <24-28°C; EEO: <0.12
RUO – Research Use Only
General References:
- James M. Bailey.; Norman Davidson. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Analytical Biochemistry. 1976, 70, (1), 75-85.
- Carl-Bertil Laurell. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Analytical Biochemistry. 1966, 15, (1), 45-52.