GeneJET 质粒中提试剂盒

Name: GeneJET 质粒中提试剂盒
SKU: K0481

Thermo Scientific GeneJET 质粒中提试剂盒足够用于 100 次制备，专为从重组大肠杆菌培养物中大规模分离高质量质粒 DNA 而设计。试剂盒采用便捷离心柱形式的硅胶膜技术。每次制备可回收高达了解更多信息

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货号	数量
K0481	25 次制备
K0482	100 次制备

2 选项

货号 K0481

价格（CNY)

数量:

25 次制备

Thermo Scientific GeneJET 质粒中提试剂盒足够用于 100 次制备，专为从重组大肠杆菌培养物中大规模分离高质量质粒 DNA 而设计。试剂盒采用便捷离心柱形式的硅胶膜技术。每次制备可回收高达 200 μg 的高拷贝数质粒 DNA。纯化质粒 DNA 可用于各种分子生物学程序，例如限制性核酸内切酶酶切、PCR、克隆、转化、自动测序、体外转录和稳健细胞系转染。

产品优势

•高效—在 50 mL 细菌培养物中获得高达 200 μg 的高质量质粒 DNA
•高浓度的纯化 DNA—> 400 ng/μL
• 便捷—离心柱形式、离心或真空方案
•快速—程序需 60 分钟，比使用重力流柱快得多

应用

•分离的质粒 DNA 适用于所有常规分子生物学程序，包括：
•FastDigest 限制性内切酶酶切
•自动荧光测序
•PCR
•克隆
•转化
•体外转录
•稳健细胞系转染

仅供科研使用。不可用于诊断程序。

规格

色谱柱类型离心柱

最大浓度400 ng/µL

适用于（应用）实时荧光定量 PCR (qPCR)、转染、克隆、测序、体外转录、转化、核酸标记、PCR

高通量能力不兼容高通量应用（手动）

制备规模100-200 μg（中等规模）质粒 DNA

产品线GeneJET

产品类型质粒 MidiPrep 试剂盒

数量25 次制备

样品类型细菌

规格中型胶

靶标质粒 DNA

测试时间60 min。

产品规格离心柱

分离技术硅胶柱

Unit SizeEach

常见问题解答 (FAQ)

Can I use water instead of Elution Buffer in the elution step of GeneJET and MagJET nucleic acid purification kits?

We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.

Are MagJET and GeneJET plasmid purification kits compatible with EndA+ bacterial strains?

Yes, MagJET and GeneJET Plasmid DNA Purification Kits are suitable for plasmid DNA purification from EndA+ strains. Please refer to the specific kit protocol for details. GeneJET Plasmid Miniprep Kit requires Wash Solution I (Cat. No. R1611, not included in the kit) for EndA+ strains.

Can I use a vacuum manifold with GeneJET kits?

The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.

At what temperature should I perform centrifugation steps in the GeneJET kits?

We recommend performing centrifugation steps at room temperature when the temperature is not specified.

Are GeneJET spin columns or MagJET magnetic beads available separately?

No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.

View all GeneJET 质粒中提试剂盒 FAQs