GeneJET Plasmid Maxiprep Kit, 10 preps - FAQs

View additional product information for GeneJET Plasmid Maxiprep Kit - FAQs (K0491, K0492)

8 product FAQs found

Can I use water instead of Elution Buffer in the elution step of GeneJET and MagJET nucleic acid purification kits?

We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.

Are MagJET and GeneJET plasmid purification kits compatible with EndA+ bacterial strains?

Yes, MagJET and GeneJET Plasmid DNA Purification Kits are suitable for plasmid DNA purification from EndA+ strains. Please refer to the specific kit protocol for details. GeneJET Plasmid Miniprep Kit requires Wash Solution I (Cat. No. R1611, not included in the kit) for EndA+ strains.

Can I use a vacuum manifold with GeneJET kits?

The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.

At what temperature should I perform centrifugation steps in the GeneJET kits?

We recommend performing centrifugation steps at room temperature when the temperature is not specified.

Are GeneJET spin columns or MagJET magnetic beads available separately?

No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.

How can I increase plasmid DNA yield during elution from GeneJET plasmid kits?

To increase the overall plasmid DNA yield by 20-30%, additional elution steps may be performed.

Which culture media are recommended for bacteria to be used with GeneJET Plasmid Kits? Can I use a rich growth medium?

The GeneJET Plasmid purification protocols are optimized for bacterial cultures grown in Luria-Bertani (LB) medium. The use of rich growth media is not recommended.

Which bacterial strains can be used for plasmid purification with GeneJET Plasmid KitS?

High quality plasmid DNA can be obtained from various E. coli strains including DH10B, DH5a, XL1-Blue, JM109, JM107, and TOP10.