GeneJET Plasmid Maxiprep Kit
GeneJET Plasmid Maxiprep Kit
Thermo Scientific™

GeneJET Plasmid Maxiprep Kit

Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column.
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Catalog NumberNo. of Reactions
K049225 Preps
K049110 Preps
Catalog number K0492
Price (CNY)
-
No. of Reactions:
25 Preps

Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers greater than 400 ng/μL of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

  • Efficient—high yields of greater than 400 ng/μL of high quality plasmid DNA from 250 mL of bacterial culture
  • High concentration of DNA purified— >400 ng/μL
  • Convenient—spin column format, centrifuge or vacuum protocols
  • Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:

  • Transfection of robust cell lines
  • FastDigest restriction enzyme digestion
  • Automated fluorescent sequencing
  • PCR
  • Cloning
  • Transformation
  • In vitro transcription
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Column TypeSpin Column
Concentration400 ng/μL
For Use With (Application)Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
High-throughput CompatibilityNot High-throughput Compatible (Manual)
No. of Reactions25 Preps
Prep Scale> 200 μg (Large-Scale) Plasmid DNA
Product LineGeneJET
Product TypePlasmid MaxiPrep Kit
Quantity25 preps
Sample TypeBacteria
ScaleMaxi
TargetPlasmid DNA
Test Time60 min.
FormatSpin Column
Isolation TechnologySilica Column
Unit SizeEach

Frequently asked questions (FAQs)

Can I use water instead of Elution Buffer in the elution step of GeneJET and MagJET nucleic acid purification kits?

We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.

Are MagJET and GeneJET plasmid purification kits compatible with EndA+ bacterial strains?

Yes, MagJET and GeneJET Plasmid DNA Purification Kits are suitable for plasmid DNA purification from EndA+ strains. Please refer to the specific kit protocol for details. GeneJET Plasmid Miniprep Kit requires Wash Solution I (Cat. No. R1611, not included in the kit) for EndA+ strains.

Can I use a vacuum manifold with GeneJET kits?

The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.

At what temperature should I perform centrifugation steps in the GeneJET kits?

We recommend performing centrifugation steps at room temperature when the temperature is not specified.

Are GeneJET spin columns or MagJET magnetic beads available separately?

No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.

View all GeneJET Plasmid Maxiprep Kit FAQs