GeneJET PCR 纯化试剂盒
Invitrogen17万+抗体限时买二赠一，靶点广，灵活用！
We have updated the storage temperature of the purification columns for this product. For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
GeneJET PCR 纯化试剂盒
Thermo Scientific™

GeneJET PCR 纯化试剂盒

Thermo Scientific GeneJET PCR 纯化试剂盒采用便捷离心柱形式的专有硅胶膜技术，无需使用昂贵的树脂或有毒的苯酚-氯仿提取。它可有效去除 PCR 和其他反应混合物中的引物、dNTP、未掺入的标记核苷酸了解更多信息
Have Questions?
更改视图buttonViewtableView
货号数量
K0702250 次制备
K070150 次制备
货号 K0702
价格（CNY)
-
数量:
250 次制备
Thermo Scientific GeneJET PCR 纯化试剂盒采用便捷离心柱形式的专有硅胶膜技术，无需使用昂贵的树脂或有毒的苯酚-氯仿提取。它可有效去除 PCR 和其他反应混合物中的引物、dNTP、未掺入的标记核苷酸、酶和盐。

该试剂盒可用于纯化 25 bp 至 20 kb 的 DNA 片段，回收率高达 100%。每个 GeneJET 纯化柱的总结合能力高达 25 μg DNA，全过程只需 5 分钟。纯化得到的 DNA 可用于常见下游应用，包括测序、限制性酶切、标记、连接、克隆、体外转录、印迹或原位杂交等。

产品优势

• 高效：在100 bp 至 10 kb的范围内 ，回收率为90% 至 100%
• 快速：程序仅需 5 分钟
• 便捷：离心柱带盖并装配收集管

应用

•快速高效纯化 DNA 片段，是所有常规分子生物学程序的理想选择，包括：
•FastDigest 或常规限制性酶切
•自动荧光或放射测序
• PCR
• 体外转录
•原位杂交
•标记
•克隆
仅供科研使用。不可用于诊断程序。
规格
最大洗脱体积50 μL
最终产品类型DNA
产品规格离心柱
高通量能力不兼容高通量应用（手动）
分离技术离心柱
纯化时间5 min。
数量250 次制备
运行时间5分钟
样品类型dsDNA
运输条件Room Temperature
原始材料体积≤400 μL
产量25 μg (Binding capacity)
适用于（应用）DNA测序, 克隆, DNA Sequencing, Nucleic Acid Hybridization, Nucleic Acid Hybridization
Unit SizeEach
内容与储存
For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.

常见问题解答 (FAQ)

Can I use water instead of Elution Buffer in the elution step of GeneJET and MagJET nucleic acid purification kits?

We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.

Can I use a vacuum manifold with GeneJET kits?

The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.

Can I use GeneJET PCR Purification Kit for DNA gel extraction?

GeneJET PCR Purification Kit is designed for PCR reaction clean-up. The kit can be used for gel extraction by following the protocol of GeneJET Gel Extraction Kit, but DNA yield may be compromised. For best performance of DNA gel extraction, we recommend to use GeneJET Gel Extraction Kit. For a 3-in-1 kit for purification of PCR products from enzymatic reaction mixtures and agarose gels, we recommend GeneJET Gel Extraction and DNA Cleanup Micro Kit (Cat. Nos. K0831/2).

Can I use GeneJET PCR Purification Kit for enzymatic clean-up?

GeneJET PCR Purification Kit can be used for enzymatic clean-up. Please follow the PCR reaction clean-up protocol.

At what temperature should I perform centrifugation steps in the GeneJET kits?

We recommend performing centrifugation steps at room temperature when the temperature is not specified.

View all GeneJET PCR 纯化试剂盒 FAQs