GeneJET 稳定和新鲜全血 RNA 试剂盒
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We have updated the storage temperature of the purification columns in this product. For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
GeneJET 稳定和新鲜全血 RNA 试剂盒
Thermo Scientific™

GeneJET 稳定和新鲜全血 RNA 试剂盒

Thermo Scientific GeneJET™ 稳定和新鲜全血 RNA 试剂盒设计用于从各种全血样品(新鲜、冷冻或使用商业稳定剂如 Tempus™了解更多信息
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货号数量
K087150 preps
货号 K0871
价格(CNY)
-
数量:
50 preps
Thermo Scientific GeneJET™ 稳定和新鲜全血 RNA 试剂盒设计用于从各种全血样品(新鲜、冷冻或使用商业稳定剂如 Tempus™ 和 PAXgene™ 稳定的人血样)中快速高效地纯化高质量的总 RNA。

试剂盒采用方便的离心柱形式的硅胶膜技术,无需使用昂贵的树脂、有毒的苯酚-氯仿提取或耗时的酒精沉淀。试剂盒包括 DNase I,用于酶法去除基因组 DNA。可以在不到 30 分钟的时间内从全血中分离出 RNA(包括细胞裂解和 DNase I 处理)。

产品优势

通用型—与新鲜和冷冻血液、血沉棕黄层以及 PAXgene™ 和 Tempus™管中采集的血液兼容
便利—特别优化的裂解缓冲液适用于 RNA 稳定长达 48 h
高效—从 500 μL 健康人血液中获得高达 4 μg 的总 RNA
纯净—分离完整的总 RNA,不含任何 RT-qPCR 抑制剂

应用

分离的总 RNA 可用于:
•RT-PCR
• RT-qPCR
•Northern 印迹

包括
•裂解缓冲液 (R2841)
•洗涤缓冲液 1(浓缩)(R2851)
•洗涤缓冲液2(浓缩)(R2861)
•DNase I(冻干)
•DNase I 重组缓冲液
• 2x DNase I 缓冲液
•氯化锰溶液
•水,无核酸酶
•预装收集管的 GeneJET RNA 纯化柱
•收集管 (2 mL)
•收集管 (1.5 mL)

相关产品
GeneJET 血液 RNA 试剂盒的裂解缓冲液
GeneJET 血液 RNA 试剂盒的洗涤缓冲液 1(浓缩液)
GeneJET 血液 RNA 试剂盒的洗涤缓冲液 2(浓缩液)
仅供科研使用。不可用于诊断程序。
规格
最大浓度2X
适用于(应用)实时荧光定量 PCR (qPCR)、逆转录酶 PCR (RT-PCR)、核酸酶保护试验、核酸标记、Northern 印迹、cDNA 文库构建
高通量能力不兼容高通量应用(手动)
反应次数50 次制备
产品线GeneJET
纯化时间30 min。
数量50 preps
产品规格离心柱
分离技术离心柱
样品类型血液
目标总 RNA
Unit SizeEach
内容与储存
For better long-term performance, it is recommended to store the purification columns at 2°C-8°C.

常见问题解答 (FAQ)

Can I use water instead of Elution Buffer in the elution step of GeneJET and MagJET nucleic acid purification kits?

We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.

Is any GeneJET kit suitable for RNA purification from formalin-fixed paraffin-embedded FFPE tissues?

We cannot recommend GeneJET kits for RNA purification from FFPE tissues. In case of genomic DNA purififcation from formalin-fixed paraffin-embedded samples, we recommend GeneJET FFPE DNA Purification Kit.

Are MagJET and GeneJET blood nucleic acid purification kits compatible with animal blood?

Yes, the kits are compatible with animal blood for DNA/RNA purification.

Can I use a vacuum manifold with GeneJET kits?

The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.

At what temperature should I perform centrifugation steps in the GeneJET kits?

We recommend performing centrifugation steps at room temperature when the temperature is not specified.