Comparison of seven different heterologous protein expression systems for the production of the serotonin transporter.
AuthorsTate CG, Haase J, Baker C, Boorsma M, Magnani F, Vallis Y, Williams DC,
JournalBiochim Biophys Acta
PubMed ID12586388
'The rat serotonin transporter (rSERT) is an N-glycosylated integral membrane protein with 12 transmembrane regions; the N-glycans improve the ability of the SERT polypeptide chain to fold into a functional transporter, but they are not required for the transmembrane transport of serotonin per se. In order to define the best ... More
Effect of some aldoses on growth of Saccharomyces cerevisiae inhibited with molybdenum.
AuthorsZemek J, Bílik V, Zákutná L,
JournalFolia Microbiol (Praha)
PubMed ID285
The inhibitory effect of molybdenum ions on growth of yeasts at pH 5.5 was found to be decreased by aldoses in the following order: D-talose greater than L-mannose greater than L-ribose greater than D-lyxose greater than L-galactose greater than L-arabinose greater than L-glucose greater than L-xylose. Increased concentrations of molybdenum ... More
Human TRPC5 channel activated by a multiplicity of signals in a single cell.
Here we explore the activation mechanisms of human TRPC5, a putative cationic channel that was cloned from a region of the X chromosome associated with mental retardation. No basal activity was evident but activity was induced by carbachol stimulation of muscarinic receptors independently of Ca2+ release. This is 'receptor activation', ... More
The gamma -secretase-cleaved C-terminal fragment of amyloid precursor protein mediates signaling to the nucleus.
Authors Gao Y; Pimplikar S W;
JournalProc Natl Acad Sci U S A
PubMed ID11742091
Sequential processing of the amyloid precursor protein (APP) by beta- and gamma-secretases generates the Abeta peptide, a major constituent of the senile plaques observed in Alzheimer's disease. The cleavage by gamma-secretase also results in the cytoplasmic release of a 59- or 57-residue-long C-terminal fragment (Cgamma). This processing resembles regulated intramembrane ... More