CCF2-AM
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CCF2-AM

CCF2-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF2 substrate which allows it to readily enter cells.了解更多信息
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货号数量
K10235 mg
货号 K1023
价格（CNY)
283,677.00
Each
添加至购物车
数量:
5 mg
价格（CNY)
283,677.00
Each
添加至购物车
CCF2-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF2 substrate which allows it to readily enter cells. Upon entry, cleavage by endogenous cytoplasmic esterases rapidly converts CCF2-AM into its negatively charged form, CCF2 or CCF4, which is retained in the cytosol. CCF2 is a Fluorescence Resonance Energy Transfer (FRET) substrate which consists of a cephalosporin core linking 7-hydroxycoumarin to fluorescein. This product is to be used as a FRET-based substrate for β-lactamase as a sensitive reporter of mammalian gene expression in the GeneBLAzer® technology.
仅供科研使用。不可用于诊断程序。
规格
细胞渗透性细胞通透性
适用于（设备）流式细胞仪、微孔板读数仪
产品线GeneBLAzer™
数量5 mg
运输条件室温
底物CCF2-AM
检测方法荧光
底物属性化学底物
底物类型β-内酰胺酶底物
Unit SizeEach
内容与储存
在冰箱（-5 至 -30°C）中储存。

常见问题解答 (FAQ)

Do you offer an alternative to GeneBLAzer In Vivo Detection Kit (Cat. No. 12578134)?

The following are two alternatives to the GeneBLAzer In Vivo Detection Kit:
- LiveBLAzer FRET-B/G Loading Kit with CCF2-AM (Cat. No. K1032, K1025)
- LiveBLAzer FRET-B/G Loading Kit with CCF4-AM (Cat. No. K1095, K1096, K1030)

Concerning CCF2-AM and CCF4-AM, these molecules differ by two carbons in the bridge that links the coumarin moiety to the lactam ring. Both are in the membrane-permeable, esterified forms, and can be used for assays in intact cells. CCF4-AM has better solubility properties than CCF2-AM and is thus best suited for screening applications. In addition, CCF4-AM has slightly better FRET and thus slightly lower background than CCF2-AM.

Note: We also offer the loading solutions as a separate kit, Loading Solutions (Cat. No. K1026), and CCF2-AM is also available as a standalone product (Cat. No. K1023).